Biomimetic digestion measuring method for pig feedstuff protein digestibility
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A bionic digestion and pig feed technology is applied in the field of estimating the digestibility of feed protein, which can solve problems such as the attenuation of digestive enzyme activity and unclear digestive enzyme activity.
Active Publication Date: 2019-04-09
INST OF ANIMAL SCI CAAS
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This method realizes the accurate estimation of the digestibility of pig feed protein or amino acid in the terminal ileum by fully automatic simulating two-stage digestion of pig stomach and small intestine in vitro, and solves the problems of unclear digestive enzyme activity, attenuation of dig
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[0052] Example 1. Changes of digestive enzyme activity with digestion time during pig stomach-small intestine biomimetic digestion and the effect of supplementing digestive enzymes during the digestion stage of the small intestine
[0053] 1 Feed ingredients, diet and pretreatment
[0054] Randomly select corn (crude protein content 8.57%, crude fiber content 1.36%, crude fat content 3.03%), soybean meal (crude protein content 42.50%, crude fiber content 6.78%, crude fat content 3.22%) from my country's feed raw material market , small bran (crude protein content 16.15%, crude fiber content 9.01%, crude fat content 3.92%) as a representative sample. And prepare corn-soybean dregs type diet (composition is as follows: 67.03% of corn, 23.22% of soybean dregs, 3.20% of wheat bran, 2.98% of soybean oil, 3.57% of multi-dimensional multi-mineral; 17.43% of crude protein content, 2.90% of crude fiber content, Crude fat content 4.80%). The above four samples were pulverized with a un...
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[0089] Example 2: Determination of the Effect of Protein Digestibility of Common Pig Feed Raw Materials by Biomimetic Digestion
[0090] 1 Pretreatment of feed ingredients
[0091] Corn, soybean meal, small bran and cottonseed meal were randomly selected as representative samples for pig feed. After pulverizing with a universal pulverizer and passing through a 60-mesh standard sieve, vacuumize-fill with nitrogen according to Example 1, pack them in sample bags and store them in a -15°C freezer for later use.
[0092] 2 Biomimetic digestion method for pigs
[0093] Cut the dialysis tubing (molecular weight cut-off 14000 Dalton, flat diameter 44mm) into small sections of about 22-28cm, put in sodium bicarbonate concentration of 2% (W / V) and disodium edetate concentration of 1mmol / L pH = 8.0 in the mixed solution. After heating to boiling on the electric ceramic hob, keep it at a slight boil for 10 minutes. After pouring off the mixed solution, wash the dialysis bag with dis...
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Abstract
The invention discloses an in vitro biomimetic digestion method for rapidly measuring the pig feedstuff protein digestibility, comprising the following steps: pulverizing feedstuff samples and the pulverized feedstuff samples passing through a standard sieve; preparing gastric buffer liquid and intestinal buffer liquid; preparing simulated gastric juice, concentrated simulated intestinal juice, concentrated supplementing intestinal juice; filling the gastric buffer liquid and the intestinal buffer liquid into an insulating chamber for the biomimetic digestion of monogastric animals, and connecting a pipeline communicating with a simulated digester; loading the pulverized feedstuff samples into the simulated digester, and then adding the simulated gastric juice, and putting into the biomimetic digestive system of monogastric animals. The in vitro biomimetic digestion process of the pig feedstuff protein is automatically completed by setting up a control software of the biomimetic digestive system for biomimetic digestion parameters at each stage of the stomach and the intestine; after obtaining the undigested residue, the crude protein content of the residue and the feedstuff samples is determined after drying, and the protein digestibility is calculated. The method is simple, high in precision and low in implementation cost, and can accurately measure the protein digestibilityof pig feedstuff within 48 hours.
Description
technical field [0001] The invention relates to a bionic digestion method for measuring the protein digestibility of pig feed, in particular to estimating the digestibility of feed protein by fully automatic externally simulating the internal digestion process of pigs under laboratory conditions. Background technique [0002] The digestible protein and amino acid content of the feed is the main decision-making basis for guiding enterprises to establish feed raw material protein titer database and formula standards, tender procurement, and optimize feed formula. It is also an important technology for reducing nitrogen excretion of livestock and poultry from the source in my country support. At present, in the production of compound feed, the data are not actual measured data, but look-up tables or empirical corrections based on crude protein content, which have large deviations and even misleading, and have become the top hidden waste in the feed industry. For nearly half a c...
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