30results about How to "Rapid Specific Test" patented technology

The invention discloses a lead ion specific detection sensor and a preparation method and a using method thereof. The sensor comprises a three-electrode system, wherein a glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as a working electrode of the system, a platinum sheet is used as a counter electrode, and a calomel electrode is used as a reference electrode. When in detection, in the three-electrode system in which the glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as the working electrode, the platinum sheet is used as the counter electrode and the calomel electrode is used as the reference electrode, an electrochemical workstation is used for performing constant-potential lead ion pre-enrichment; and after the enrichment is finished, the lead ion is detected by a differential pulse working mode. Through the detection sensor, simple and quick specific detection on the lead ion is realized; the lead ion with concentration as low as 0.01ug/L can be detected by the method; meanwhile, the lead ion sensor is simple to prepare, has stable performance and can be repeatedly used; and the sample detection time is short, and the operation is convenient.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention provides a real-time fluorescent PCR reagent kit for screening active Listeria monocytogenes. The real-time fluorescent PCR reagent kit mainly comprises specific primers, a fluorescent probe, PCR buffer solution, deoxidant nucleoside triphosphate mixture and DNA polymerase. The sequence of the specific primers and the fluorescent probe is as follows: an upstream primer: 5'- CGGAGGTTCCGCAAAAGATG-3', a downstream primer: 5' -CCTCCAGAGTGATCGATGTT-3' and the fluorescent probe: 5' FAM-AAATCATCGACGGCAACCTC-TAMRA-3', wherein, the FAM is a fluorescent reporter group, and the TAMRA is a fluorescent quenching group. The real-time fluorescent PCR reagent kit has the beneficial effects that the real-time fluorescent PCR reagent kit can effectively screen the life and death state of bacteria and has the high sensitivity and the good specificity of the detection of bacteria, thereby reducing the false positive rate of regular PCR amplification; and the real-time fluorescent PCR reagent kit can realize fast, exact and specific detection towards the active Listeria monocytogenes.

A gene chip for detecting the resistance of hepatitis B virus to lamivudine has a thin glass substrate with multiple reaction areas. Each reaction area has a detection microarray consisting of 4 subarrays. Each subarrray has 30 probes composed of 28 probes for detecting the DNA polymerases of HBV, a HBV DNA probe and a negative reference probe. Its preparing process includes preparing relative reagents, preparing gene chip, and providing various kinds of solution. Its detection method includes PCR amplification, marking, hybridizing with said probes, washing, laser scanning to obtain images, converting image to digital values, and data processing.

The invention discloses a human body helicobacter pylori infection diagnosing system built on the basis of fuel cells and a method for detecting the content of ammonia in human respiratory gas. The human body helicobacter pylori infection diagnosing system comprises oral urea preparations and a fuel cell type ammonia detecting device. The human body helicobacter pylori infection diagnosing system and the method have the advantages that the human expiratory gas is led into the fuel cell type ammonia detecting device after human bodies administer the oral urea preparations, current signals which indicate that oxidation and reduction reaction is carried out on the ammonia in the human respiratory gas in fuel cell systems are detected, accordingly, the content of the ammonia in the human respiratory gas can be quickly and accurately obtained, the helicobacter pylori infection degrees can be sensitively, quickly and specifically detected, toxic and side effects and service limitation can be reduced to a great extent as compared with an existing diagnosing method, and the detection time can be greatly shortened as compared with the exiting diagnosing method.

The invention discloses application of a specific target primer in simultaneous and rapid identification of two pathogenic bacteria infected by strawberry. The specific target primer is composed of LAMP primer sequences of a fusarium oxysporum IGS gene and a strawberry colletotrichum ITS gene. The method comprises the following steps: designing an LAMP primer of a specific ITS sequence of fusariumoxysporum, an LAMP primer of a CYP sequence, an LAMP primer of an IGS sequence and other technical means, and finally selecting the IGS sequence to design the LAMP primer for identifying strawberry fusarium oxysporum. Meanwhile, the characteristic of extremely low homology between the rear half part of the colletotrichum strain ITS and other genera is utilized, an LAMP specific primer of an ITS sequence is designed; meanwhile, an ITS primer and an IGS primer are added, fusarium oxysporum and colletotrichum gloeosporioides in strawberries are rapidly and accurately distinguished and detected for the first time through the LAMP technology, the detection sensitivity is improved by 1000 times compared with that of a PCR technology, the price is low, the detection time is short, the method issimple, and the LAMP technology plays an important role in real-time monitoring and early warning of strawberry pathogenic bacteria.

The invention relates to the field of food, in particular to a kit and an application thereof as well as a detection method. According to the invention, a method for detecting live cronobacter sakazakii in infant formula milk powder by using an EMA fluorescent quantitative PCR technology; a sample of infant formula milk powder polluted by cronobacter sakazakii is subjected to double-blind detection and simulation, and the tests for the removal rate of dead bacteria DNA, sensitivity and specificity indicate that the method is feasible. According to the method, EMA is used for the pretreatment of the detected sample, so as to avoid the false positive rate caused by dead bacteria or residual DNA in conventional PCR amplification and greatly improve the specificity. Moreover, the fluorescent PCR amplification technology of TaqMan-MGB probe is adopted, so as to greatly improve the bacterial sensitivity and specificity.

The invention provides a nano immunosensor for detecting penicillin G residues in a dairy product based on a double-layer lipoid membrane and a preparation method of the nano immunosensor. The nano immunosensor is composed of a working electrode, the double-layer lipoid membrane, Ag@Au core-shell nanoparticles and a penicillin G antibody. The nano immunosensor has the advantages of being simple toprepare, high in sensitivity, rapid in detection, strong in specificity, simple and convenient to operate, capable of directly detecting signals, good in detection limit and the like; the defects that an existing biosensor for penicillin G detection is tedious in preparation process, complex in equipment and free of sufficiently low detection limit are overcome; and construction of an immunosensor for detecting penicillin G by adopting an unlabeled immunoelectrochemistry method is realized.

The invention discloses a specific nitrite fluorescence detection method based on a copper nano-cluster and an oxidation reaction, and belongs to the technical field of nano-material preparation and nitrite detection. The method comprises the following steps: firstly, preparing a copper nano-cluster by using glutathione as a stabilizer and ascorbic acid as a reducing agent through a one-pot method, and constructing a fluorescence sensor for detecting nitrite on the basis of an oxidation-reduction reaction; under an acidic condition, Fe < 2 + > can be easily oxidized into Fe < 3 + > due to existence of NO2, and quantitative analysis of NO2 <-> can be easily realized by combining a Fe < 2 + >/Fe < 3 + > oxidation-reduction process with fluorescence quenching of Fe < 3 + > on Cu NCs. According to the invention, the sensitivity and accuracy of nitrite detection can be improved, and the sensor has good specificity.