30results about How to "Rapid Specific Test" patented technology

The application discloses a method and primers for detecting index sequence contamination of a sequencing platform. The method for detecting contamination of the index sequence of the sequencing platform of the present application includes using specific primers for the contamination index sequence to perform PCR amplification on all sub-libraries of the contaminated sequencing channel, and according to whether there is specific amplification in the PCR amplification result , to judge whether there is index sequence pollution in the corresponding sub-library, so as to detect the pollution source of the index sequence of the sequencing platform. The index sequence contamination detection method of the sequencing platform of the present application provides a brand-new solution and idea for the detection of index sequence contamination on the sequencing platform, which can quickly and specifically detect the source of index sequence contamination, and has low cost, high accuracy, and easy operation. Simple and convenient, easy to promote and use.

The invention provides a nano immunosensor for detecting penicillin G residues in a dairy product based on a double-layer lipoid membrane and a preparation method of the nano immunosensor. The nano immunosensor is composed of a working electrode, the double-layer lipoid membrane, Ag@Au core-shell nanoparticles and a penicillin G antibody. The nano immunosensor has the advantages of being simple toprepare, high in sensitivity, rapid in detection, strong in specificity, simple and convenient to operate, capable of directly detecting signals, good in detection limit and the like; the defects that an existing biosensor for penicillin G detection is tedious in preparation process, complex in equipment and free of sufficiently low detection limit are overcome; and construction of an immunosensor for detecting penicillin G by adopting an unlabeled immunoelectrochemistry method is realized.

The invention relates to a method for preparing an alpha fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduced graphene and belongs to the field of electrochemiluminescence sensors. Tris bipyridine ruthenium is taken as the electrochemiluminescence signal source, and the carrier content of an antibody is increased by means of the excellent biocompatibility and large specific surface area of gold hybrid carbon black intercalation reduced graphene. Nano-porous silver is taken as the second antibody marker, and the hepatoma marker alpha fetoprotein is detected based on the fact that electrochemiluminescence signal intensity changes as the concentration of tested substances changes.

The invention provides a real-time fluorescence PCR ( Polymerase Chain Reaction) kit for detecting shigella and a detection method thereof. The kit mainly comprises specific primers, a fluorescent probe, PCR buffer liquid, deoxynucleotide triphosphate mixture and DNA polymerase. The sequences of the specific primers and fluorescent probe are as follows: an upstream primer has the sequence of 5'-GGAAAACCCTCCTGGTCCAT-3', a downstream primer has the sequence of 5'-CGCCGGTATCATTATCGAAAA-3', and the fluorescent probe has the sequence of 5'-FAM-AGGCATCAGAAGGC-MGB-3', wherein the FAM is a fluorescent report radical, and the MGB is a fluorescent quenching radical. The invention has the advantages that the detection sensitivity of target bacteria is high, the specificity is good, the false positive rate of regular PCR amplification is reduced, and the shigella can be rapidly, accurately and peculiarly detected.

The invention discloses a kit for detecting vibrio parahaemolyticus on the basis of immunomagnetic beads and MnO2 nanometer particles. Magnetic beads with superparamagnetism are prepared; the magnetic beads are coupled with vibrio parahaemolyticus polyclonal antibodies; MnO2 nanometer particles are synthesized; the nanometer particles are coupled with vibrio parahaemolyticus specific chicken egg-yolk antibodies; liquid to be tested is taken and is mixed with two kinds of probes; a magnetic force frame is used for separating magnetic beads-thallus-MnO2 compounds; a citrate buffer solution is used for resuspending the mixture; TMB is added for color development, so that the fast specific detection of the vibrio parahaemolyticus is realized. The vibrio parahaemolyticus detection by using the immunomagnetic beads and MnO2 nanometer particle technology is provided by the invention; the immunological reaction of an ELISA method is used for color development; the detection time is shortened; during the quantitative detection, the variation coefficient is small; the lowest detection concentration is 10 CFU / mL; the adding standard recovery rate reaches 96.7 percent; the sensitivity is high; the stability is high.