30results about How to "Rapid Specific Test" patented technology

The invention discloses a lead ion specific detection sensor and a preparation method and a using method thereof. The sensor comprises a three-electrode system, wherein a glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as a working electrode of the system, a platinum sheet is used as a counter electrode, and a calomel electrode is used as a reference electrode. When in detection, in the three-electrode system in which the glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as the working electrode, the platinum sheet is used as the counter electrode and the calomel electrode is used as the reference electrode, an electrochemical workstation is used for performing constant-potential lead ion pre-enrichment; and after the enrichment is finished, the lead ion is detected by a differential pulse working mode. Through the detection sensor, simple and quick specific detection on the lead ion is realized; the lead ion with concentration as low as 0.01ug / L can be detected by the method; meanwhile, the lead ion sensor is simple to prepare, has stable performance and can be repeatedly used; and the sample detection time is short, and the operation is convenient.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention discloses a kit for detecting vibrio parahaemolyticus on the basis of immunomagnetic beads and MnO2 nanometer particles. Magnetic beads with superparamagnetism are prepared; the magnetic beads are coupled with vibrio parahaemolyticus polyclonal antibodies; MnO2 nanometer particles are synthesized; the nanometer particles are coupled with vibrio parahaemolyticus specific chicken egg-yolk antibodies; liquid to be tested is taken and is mixed with two kinds of probes; a magnetic force frame is used for separating magnetic beads-thallus-MnO2 compounds; a citrate buffer solution is used for resuspending the mixture; TMB is added for color development, so that the fast specific detection of the vibrio parahaemolyticus is realized. The vibrio parahaemolyticus detection by using the immunomagnetic beads and MnO2 nanometer particle technology is provided by the invention; the immunological reaction of an ELISA method is used for color development; the detection time is shortened; during the quantitative detection, the variation coefficient is small; the lowest detection concentration is 10 CFU / mL; the adding standard recovery rate reaches 96.7 percent; the sensitivity is high; the stability is high.

The invention discloses a real-time fluorescence quantitative polymerase chain reaction (PCR) kit for five kinds of pathogens such as incorporeity, rickettsia and the like and a method thereof. Specific oligonucleotide primers and probes are designed according to the specific gene nucleotide sequences of the five kinds of pathogens such as the incorporeity, the rickettsia and the like. The invention provides a method for detecting the incorporeity and the rickettsia and a high-sensitivity detection kit consisting of the specific primers. The kit is easy, convenient and quick in operation, andhas high sensitivity and specificity. The kit can perform rapid specific detection on 5 kinds of related rickettsia and is applicable to clinical laboratories and disease monitoring tissue laboratories. The invention also discloses a method for detecting the incorporeity and the rickettsia by using the kit.

The invention provides a real-time fluorescence PCR ( Polymerase Chain Reaction) kit for detecting shigella and a detection method thereof. The kit mainly comprises specific primers, a fluorescent probe, PCR buffer liquid, deoxynucleotide triphosphate mixture and DNA polymerase. The sequences of the specific primers and fluorescent probe are as follows: an upstream primer has the sequence of 5'-GGAAAACCCTCCTGGTCCAT-3', a downstream primer has the sequence of 5'-CGCCGGTATCATTATCGAAAA-3', and the fluorescent probe has the sequence of 5'-FAM-AGGCATCAGAAGGC-MGB-3', wherein the FAM is a fluorescent report radical, and the MGB is a fluorescent quenching radical. The invention has the advantages that the detection sensitivity of target bacteria is high, the specificity is good, the false positive rate of regular PCR amplification is reduced, and the shigella can be rapidly, accurately and peculiarly detected.

A gene chip for detecting the resistance of hepatitis B virus to lamivudine has a thin glass substrate with multiple reaction areas. Each reaction area has a detection microarray consisting of 4 subarrays. Each subarrray has 30 probes composed of 28 probes for detecting the DNA polymerases of HBV, a HBV DNA probe and a negative reference probe. Its preparing process includes preparing relative reagents, preparing gene chip, and providing various kinds of solution. Its detection method includes PCR amplification, marking, hybridizing with said probes, washing, laser scanning to obtain images, converting image to digital values, and data processing.

The invention discloses a phenanthroline derivative, a zinc ion fluorescence probe and application thereof. The phenanthroline derivative can serve as the zinc ion fluorescence probe and is applied tothe fields of metal ion identification and detection. The phenanthroline derivative has a structure as shown in any one of formulas (I) to (II) (the formulas are as shown in the description), whereinR comprises any one of thienyl, carbazolyl, fluorenyl, pyrenyl and 4-(2,2-distyryl)phenyl. The phenanthroline zinc ion fluorescence probe has high selectivity and high sensitivity on zinc ions, can realize rapid detection of the zinc ions, has high selectivity and high capability of resisting other ion interference, provides a new method for designing the metal ion probe with high selectivity andsensitivity, and has wide application prospect in the field of metal ion detection.

The invention discloses a human body helicobacter pylori infection diagnosing system built on the basis of fuel cells and a method for detecting the content of ammonia in human respiratory gas. The human body helicobacter pylori infection diagnosing system comprises oral urea preparations and a fuel cell type ammonia detecting device. The human body helicobacter pylori infection diagnosing system and the method have the advantages that the human expiratory gas is led into the fuel cell type ammonia detecting device after human bodies administer the oral urea preparations, current signals which indicate that oxidation and reduction reaction is carried out on the ammonia in the human respiratory gas in fuel cell systems are detected, accordingly, the content of the ammonia in the human respiratory gas can be quickly and accurately obtained, the helicobacter pylori infection degrees can be sensitively, quickly and specifically detected, toxic and side effects and service limitation can be reduced to a great extent as compared with an existing diagnosing method, and the detection time can be greatly shortened as compared with the exiting diagnosing method.

The present invention relates to a preparation method of a breast cancer susceptibility gene immune sensor based on flaky strontium ferrite double amplification, and belongs to the technical fields of novel functional materials and biological sensors. According to the present invention, based on the characteristics of large effective specific surface area, excellent catalysis activity and the like of the flaky strontium ferrite, the sensitivity and stability of the immune sensor are significantly improved, and the important significance is provided for early diagnosis of breast cancer.

The invention discloses application of a specific target primer in simultaneous and rapid identification of two pathogenic bacteria infected by strawberry. The specific target primer is composed of LAMP primer sequences of a fusarium oxysporum IGS gene and a strawberry colletotrichum ITS gene. The method comprises the following steps: designing an LAMP primer of a specific ITS sequence of fusariumoxysporum, an LAMP primer of a CYP sequence, an LAMP primer of an IGS sequence and other technical means, and finally selecting the IGS sequence to design the LAMP primer for identifying strawberry fusarium oxysporum. Meanwhile, the characteristic of extremely low homology between the rear half part of the colletotrichum strain ITS and other genera is utilized, an LAMP specific primer of an ITS sequence is designed; meanwhile, an ITS primer and an IGS primer are added, fusarium oxysporum and colletotrichum gloeosporioides in strawberries are rapidly and accurately distinguished and detected for the first time through the LAMP technology, the detection sensitivity is improved by 1000 times compared with that of a PCR technology, the price is low, the detection time is short, the method issimple, and the LAMP technology plays an important role in real-time monitoring and early warning of strawberry pathogenic bacteria.

The invention provides a fluoroscopic examination kit for detecting comma bacillus O139. The sequences of specific primers and a fluorescent probe in the kit are as follows: an upstream primer 5'-TCCTGAAATATTGTTGCGTTATAGG-3', a downstream primer 5'-ATCCCTATAGATGTAAGCACTTCA-3' and the fluorescent probe 5'-FAM-AAGAAAGATTTGAGATCATTTC-TAMRA-3', wherein FAM refers to a fluorescent reporter group, and TAMRA refers to a fluorescent quenching group. The fluoroscopic examination kit has the advantages of high bacterium detection sensitivity, good specificity, reduction of the false positive rate of general PCR amplification, and capability of realizing quick, accurate and special detection of the comma bacillus O139.

The invention relates to the field of food, in particular to a kit and an application thereof as well as a detection method. According to the invention, a method for detecting live cronobacter sakazakii in infant formula milk powder by using an EMA fluorescent quantitative PCR technology; a sample of infant formula milk powder polluted by cronobacter sakazakii is subjected to double-blind detection and simulation, and the tests for the removal rate of dead bacteria DNA, sensitivity and specificity indicate that the method is feasible. According to the method, EMA is used for the pretreatment of the detected sample, so as to avoid the false positive rate caused by dead bacteria or residual DNA in conventional PCR amplification and greatly improve the specificity. Moreover, the fluorescent PCR amplification technology of TaqMan-MGB probe is adopted, so as to greatly improve the bacterial sensitivity and specificity.

The invention is suitable for the technical field of quality detection, and provides a megastigmatrienone hapten, an artificial antigen, a preparation method of the artificial antigen, an antibody and an application of the megastigmatrienone hapten, the structural formula of the megastigmatrienone hapten is shown in the specification, and n is any integer from 2 to 4. According to the invention, the existing keto group on megastigmatrienone is used for reduction, and the megastigmatrienone has a carboxyl group coupled with a carrier protein by introducing a connecting arm. On one hand, the chemical structure and characteristics of megastigmatrienone can be reserved to the greatest extent, on the other hand, the introduced carboxyl group can be directly coupled with carrier protein, and both the carboxyl group and the carrier protein lay a foundation for subsequent preparation of high-specificity and high-sensitivity antibodies; specifically, the megastigmatrienone artificial antigen is prepared on the basis of the megastigmatrienone hapten, and an antibody obtained from the megastigmatrienone artificial antigen has high titer, specificity and affinity and can be applied to rapid and specific detection of megastigmatrienone in tobacco leaves.

The invention provides a nano immunosensor for detecting penicillin G residues in a dairy product based on a double-layer lipoid membrane and a preparation method of the nano immunosensor. The nano immunosensor is composed of a working electrode, the double-layer lipoid membrane, Ag@Au core-shell nanoparticles and a penicillin G antibody. The nano immunosensor has the advantages of being simple toprepare, high in sensitivity, rapid in detection, strong in specificity, simple and convenient to operate, capable of directly detecting signals, good in detection limit and the like; the defects that an existing biosensor for penicillin G detection is tedious in preparation process, complex in equipment and free of sufficiently low detection limit are overcome; and construction of an immunosensor for detecting penicillin G by adopting an unlabeled immunoelectrochemistry method is realized.

The invention discloses a detection method for Listeria monocytogenes. The present invention utilizes the nano-silver cage as the substrate, mixes it with the standard sample solution of Listeria monocytogenes of known concentration, and performs Raman detection after hatching to establish a standard curve of Listeria monocytogenes concentration-Raman signal intensity, and then The nano-silver cage substrate is mixed with the actual sample to be tested, and the Raman signal intensity obtained by Raman detection after incubation is compared with the standard curve, so as to obtain the content of Listeria monocytogenes in the actual sample to be tested. The invention combines the spatial limitation effect and the Raman fingerprint spectrum to realize rapid and specific detection of Listeria monocytogenes. The method is simple, does not need to prepare a template, is environmentally friendly and pollution-free, and the prepared nano-silver cage base shell and inner wall are rough, which greatly increases the contact area between Listeria monocytogenes and the base, thereby significantly improving the SERS detection effect.

The invention provides a kit for detecting salmonella typhimurium and a preparation method of the kit, and belongs to the field of kits. The kit comprises an apt-Fe3O4@Ag NPs nanoprobe of salmonella,a hydrogen peroxide etching agent, a urease solution and urea / phenol red test paper. The invention also provides a preparation method of the kit for detecting salmonella typhimurium. The salmonella typhimurium is detected by using an immunomagnetic bead and nano particle composite structure, and color development is performed through test paper by using an enzyme inhibition method, so that the detection time is shortened, the coefficient of variation during quantitative detection is small, the lowest detection concentration is 21cfu.ML<-1>, the spiked recovery rate reaches 96.18%, the sensitivity is high, and the stability is good.

The invention discloses a specific nitrite fluorescence detection method based on a copper nano-cluster and an oxidation reaction, and belongs to the technical field of nano-material preparation and nitrite detection. The method comprises the following steps: firstly, preparing a copper nano-cluster by using glutathione as a stabilizer and ascorbic acid as a reducing agent through a one-pot method, and constructing a fluorescence sensor for detecting nitrite on the basis of an oxidation-reduction reaction; under an acidic condition, Fe < 2 + > can be easily oxidized into Fe < 3 + > due to existence of NO2, and quantitative analysis of NO2 <-> can be easily realized by combining a Fe < 2 + > / Fe < 3 + > oxidation-reduction process with fluorescence quenching of Fe < 3 + > on Cu NCs. According to the invention, the sensitivity and accuracy of nitrite detection can be improved, and the sensor has good specificity.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention discloses a phenanthroline derivative, a zinc ion fluorescence probe and application thereof. The phenanthroline derivative can serve as the zinc ion fluorescence probe and is applied tothe fields of metal ion identification and detection. The phenanthroline derivative has a structure as shown in any one of formulas (I) to (II) (the formulas are as shown in the description), whereinR comprises any one of thienyl, carbazolyl, fluorenyl, pyrenyl and 4-(2,2-distyryl)phenyl. The phenanthroline zinc ion fluorescence probe has high selectivity and high sensitivity on zinc ions, can realize rapid detection of the zinc ions, has high selectivity and high capability of resisting other ion interference, provides a new method for designing the metal ion probe with high selectivity andsensitivity, and has wide application prospect in the field of metal ion detection.

The invention belongs to the technical field of agricultural product and food quality detection, and discloses a method for preparing a beta-ionone hapten, an artificial antigen and an antibody, carboxyl groups are directly introduced through aldehyde groups of beta-ionone, hydroxyl groups are generated through reduction reaction, and spacer arms with carboxyl groups are obtained. The carboxyl group is directly introduced through the hydroxyl group on the beta-ionone, the chemical structure and characteristics of the beta-ionone are reserved to the maximum extent, the beta-ionone can be directly coupled with carrier protein, and a foundation is laid for subsequent preparation of the beta-ionone antibody with high specificity and high sensitivity. Meanwhile, based on an aldehyde group in a beta-ionone structure, a hydroxyl group is directly generated through a reduction reaction, a spacer arm with a carboxyl group is generated, raw materials are relatively common, reaction operation is simple, high-temperature and high-pressure conditions are not needed, control is easy, and the prepared beta-ionone hapten is relatively high in purity and yield.

The invention is suitable for the technical field of quality detection, and provides a scopoletin hapten, a scopoletin artificial antigen, a preparation method thereof, an antibody and an application thereof. The structural formula of the scopoletin hapten is shown in the specification, and n is any integer from 2 to 4. A carboxyl group is directly introduced into a hydroxyl group of the scopoletin, the chemical structure and characteristics of the scopoletin are reserved to the maximum extent, the scopoletin can be directly coupled with carrier protein, and a foundation is laid for subsequent preparation of antibodies with high specificity and high sensitivity; moreover, the scopoletin artificial antigen is prepared on the basis of the scopoletin hapten, and an antibody obtained from the scopoletin artificial antigen has high titer, specificity and affinity and can be applied to rapid and specific detection of scopoletin in agricultural products such as tobacco leaves and related food.

The invention provides a real-time fluorescence PCR ( Polymerase Chain Reaction) kit for detecting shigella and a detection method thereof. The kit mainly comprises specific primers, a fluorescent probe, PCR buffer liquid, deoxynucleotide triphosphate mixture and DNA polymerase. The sequences of the specific primers and fluorescent probe are as follows: an upstream primer has the sequence of 5'-GGAAAACCCTCCTGGTCCAT-3', a downstream primer has the sequence of 5'-CGCCGGTATCATTATCGAAAA-3', and the fluorescent probe has the sequence of 5'-FAM-AGGCATCAGAAGGC-MGB-3', wherein the FAM is a fluorescent report radical, and the MGB is a fluorescent quenching radical. The invention has the advantages that the detection sensitivity of target bacteria is high, the specificity is good, the false positive rate of regular PCR amplification is reduced, and the shigella can be rapidly, accurately and peculiarly detected.

The invention discloses a kit for detecting vibrio parahaemolyticus on the basis of immunomagnetic beads and MnO2 nanometer particles. Magnetic beads with superparamagnetism are prepared; the magnetic beads are coupled with vibrio parahaemolyticus polyclonal antibodies; MnO2 nanometer particles are synthesized; the nanometer particles are coupled with vibrio parahaemolyticus specific chicken egg-yolk antibodies; liquid to be tested is taken and is mixed with two kinds of probes; a magnetic force frame is used for separating magnetic beads-thallus-MnO2 compounds; a citrate buffer solution is used for resuspending the mixture; TMB is added for color development, so that the fast specific detection of the vibrio parahaemolyticus is realized. The vibrio parahaemolyticus detection by using the immunomagnetic beads and MnO2 nanometer particle technology is provided by the invention; the immunological reaction of an ELISA method is used for color development; the detection time is shortened; during the quantitative detection, the variation coefficient is small; the lowest detection concentration is 10 CFU / mL; the adding standard recovery rate reaches 96.7 percent; the sensitivity is high; the stability is high.

The invention discloses a human body helicobacter pylori infection diagnosing system built on the basis of fuel cells and a method for detecting the content of ammonia in human respiratory gas. The human body helicobacter pylori infection diagnosing system comprises oral urea preparations and a fuel cell type ammonia detecting device. The human body helicobacter pylori infection diagnosing system and the method have the advantages that the human expiratory gas is led into the fuel cell type ammonia detecting device after human bodies administer the oral urea preparations, current signals which indicate that oxidation and reduction reaction is carried out on the ammonia in the human respiratory gas in fuel cell systems are detected, accordingly, the content of the ammonia in the human respiratory gas can be quickly and accurately obtained, the helicobacter pylori infection degrees can be sensitively, quickly and specifically detected, toxic and side effects and service limitation can be reduced to a great extent as compared with an existing diagnosing method, and the detection time can be greatly shortened as compared with the exiting diagnosing method.

The invention provides a kit for detecting Salmonella typhimurium and a preparation method thereof, belonging to the field of kits. The kit includes: apt‑Fe for Salmonella 3 o 4 @Ag NPs nanoprobes, hydrogen peroxide etchant, urease solution and urea / phenol red test paper. The invention also provides a preparation method of a kit for detecting Salmonella typhimurium. The invention uses the composite structure of immune magnetic beads and nanoparticles to detect Salmonella typhimurium, uses the enzyme inhibition method to develop color through test paper, shortens the detection time, has a small coefficient of variation in quantitative detection, and has a minimum detection concentration of 21cfu·mL ‑1 , the standard addition recovery rate reached 96.18%, high sensitivity and good stability.

The present invention provides a kind of beneficial endogenous carbon quantum dots. The carbon quantum dots are prepared according to the following method: Weigh precious Maojian green tea and ascorbic acid in a reaction kettle, add ultrapure water into the reaction kettle, wait until the ascorbic acid is fully After dissolving, react at 200°C for 3 hours, then cool to room temperature, centrifuge the reaction solution, take the supernatant and dialyze it in a dialysis membrane with a molecular weight cut-off of 3500 for 24 hours, filter the solution after passing through the dialysis membrane with a 0.22 μm filter membrane, and take The filtrate is decompressed and rotary evaporated, and then freeze-dried to obtain final product; the carbon dots of the present invention have the advantages of non-toxicity, good biocompatibility, stable optical properties, etc., and can treat Hg 2+ Fast, convenient and specific detection of ions.