Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of kit for detecting Salmonella typhimurium and preparation method thereof

A Salmonella, Typhimurium technology, applied in measuring devices, material analysis, instruments and other directions by observing the impact on chemical indicators, can solve the problems of unsuitable for on-site detection of samples, prone to false positives, low sensitivity, etc., and achieve shortened time. Detection time, high sensitivity and good stability

Active Publication Date: 2021-05-25
JILIN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PCR technology can amplify the detection signal in a short time and has the characteristics of high sensitivity, but the operation process of PCR requires professionals and is prone to false positives
The ELISA method is based on the specific binding of antigens and antibodies for detection, without the need for large instruments, but it takes a long time and has low sensitivity
As an emerging detection technology, LAMP has the advantages of low cost, high sensitivity and specificity, but LAMP requires selective enrichment culture, so it is not suitable for on-site detection of samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of kit for detecting Salmonella typhimurium and preparation method thereof
  • A kind of kit for detecting Salmonella typhimurium and preparation method thereof
  • A kind of kit for detecting Salmonella typhimurium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The present invention also provides a preparation method of a kit for detecting Salmonella typhimurium, comprising:

[0037] Step 1: Fe 3 o 4 Preparation of nanoparticles

[0038] FeCl 3 ·6H 2 O, sodium citrate and CH 3 COONa·3H 2 O is magnetically stirred at room temperature in ethylene glycol until it is completely dissolved, and then the resulting homogeneous solution is transferred to a reaction kettle, preferably reacted in a 200°C oven for 10 hours; the dark brown solid matter is separated from the reaction solution with a permanent magnet, and the supernatant is used. Alternately wash with pure water and absolute ethanol for 3-5 times to obtain superparamagnetic Fe 3 o 4 Nanoparticles; the FeCl 3 ·6H 2 O, sodium citrate and CH 3 COONa·3H 2 O mass ratio is preferably 2.16:0.5:3.34;

[0039] Step 2: Fe 3 o 4 Preparation of @Ag magnetic nanoparticles

[0040] Preparation of Fe by Liquid Phase Reduction 3 o 4@Ag nanoparticles, specifically: the AgNO ...

Embodiment 1

[0053] Example 1 Salmonella apt-Fe 3 o 4 Preparation of @Ag NPs

[0054] First prepare the Fe 3 o 4 @Ag, 2.16g FeCl 3 ·6H 2 O, 0.5g sodium citrate and 3.34g CH 3 COONa·3H 2 O was magnetically stirred at room temperature in 40 mL of ethylene glycol until completely dissolved. Then transfer the obtained homogeneous solution to a reaction kettle and react in an oven at 200°C for 10 hours; use a permanent magnet to separate the dark brown solid matter from the reaction solution, wash it alternately with ultrapure water and absolute ethanol for 3-5 times, and obtain Superparamagnetic Fe 3 o 4 Nanoparticles.

[0055] Preparation of Fe by Liquid Phase Reduction 3 o 4 @Ag nanoparticles, 0.1g AgNO 3 and 2.5g polyvinylpyrrolidone k-30 were dissolved in 24mL polyethylene glycol, after fully dissolving, add 0.2g prepared Fe 3 o 4 Nanoparticles were dispersed in it, stirred at 100°C for 12h, the brown solid matter was separated from the reaction solution with a permanent mag...

Embodiment 2

[0057] The preparation of embodiment 2 urea / phenol red test paper:

[0058] Dissolve 0.1 g of phenol red and 3 g of urea in 60% ethanol solution. Soak neutral cotton filter paper in the above solution for 10 minutes at room temperature. The soaked test paper was laid flat and dried in an oven at 37°C. Store the prepared urea / phenol red test paper in a dry, sealed, cool and light-proof environment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a kit for detecting Salmonella typhimurium and a preparation method thereof, belonging to the field of kits. The kit includes: apt‑Fe for Salmonella 3 o 4 @Ag NPs nanoprobes, hydrogen peroxide etchant, urease solution and urea / phenol red test paper. The invention also provides a preparation method of a kit for detecting Salmonella typhimurium. The invention uses the composite structure of immune magnetic beads and nanoparticles to detect Salmonella typhimurium, uses the enzyme inhibition method to develop color through test paper, shortens the detection time, has a small coefficient of variation in quantitative detection, and has a minimum detection concentration of 21cfu·mL ‑1 , the standard addition recovery rate reached 96.18%, high sensitivity and good stability.

Description

technical field [0001] The invention belongs to the field of kits, in particular to a kit for detecting Salmonella typhimurium and a preparation method thereof. Background technique [0002] Salmonella typhimurium (S. typhimurium), a Gram-negative enteric bacterium, is one of the most common pathogens responsible for foodborne illness outbreaks. Salmonella usually contaminates food such as eggs, fruits and vegetables, meat and milk through animal feces. Salmonella infection can cause typical gastroenteritis reactions, such as abdominal pain, diarrhea, nausea, vomiting, fever, and other symptoms of gastroenteritis. According to reports, food poisoning caused by Salmonella is still on the rise. At present, this bacterium is a must-check pathogen for agricultural and sideline products such as eggs, meat and milk. [0003] At present, the traditional detection method of Salmonella in my country is mainly based on microbial culture method, which takes 3-5 days for the whole pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/543G01N21/78
CPCG01N21/78G01N33/54326G01N33/54346G01N33/56916G01N2021/7759G01N2333/255
Inventor 王娟赵超魏胜男
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com