A kind of kit for detecting Salmonella typhimurium and preparation method thereof

Abstract

The invention provides a kit for detecting Salmonella typhimurium and a preparation method thereof, belonging to the field of kits. The kit includes: apt‑Fe for Salmonella 3 o 4 @Ag NPs nanoprobes, hydrogen peroxide etchant, urease solution and urea / phenol red test paper. The invention also provides a preparation method of a kit for detecting Salmonella typhimurium. The invention uses the composite structure of immune magnetic beads and nanoparticles to detect Salmonella typhimurium, uses the enzyme inhibition method to develop color through test paper, shortens the detection time, has a small coefficient of variation in quantitative detection, and has a minimum detection concentration of 21cfu·mL ‑1 , the standard addition recovery rate reached 96.18%, high sensitivity and good stability.

Description

technical field

[0001] The invention belongs to the field of kits, in particular to a kit for detecting Salmonella typhimurium and a preparation method thereof. Background technique

[0002] Salmonella typhimurium (S. typhimurium), a Gram-negative enteric bacterium, is one of the most common pathogens responsible for foodborne illness outbreaks. Salmonella usually contaminates food such as eggs, fruits and vegetables, meat and milk through animal feces. Salmonella infection can cause typical gastroenteritis reactions, such as abdominal pain, diarrhea, nausea, vomiting, fever, and other symptoms of gastroenteritis. According to reports, food poisoning caused by Salmonella is still on the rise. At present, this bacterium is a must-check pathogen for agricultural and sideline products such as eggs, meat and milk.

[0003] At present, the traditional detection method of Salmonella in my country is mainly based on microbial culture method, which takes 3-5 days for the whole pr...

Images