Nano-immunosensor for detection of penicillin g residues in dairy products and its preparation method

Abstract

The invention provides a nano immunosensor for detecting penicillin G residues in a dairy product based on a double-layer lipoid membrane and a preparation method of the nano immunosensor. The nano immunosensor is composed of a working electrode, the double-layer lipoid membrane, Ag@Au core-shell nanoparticles and a penicillin G antibody. The nano immunosensor has the advantages of being simple toprepare, high in sensitivity, rapid in detection, strong in specificity, simple and convenient to operate, capable of directly detecting signals, good in detection limit and the like; the defects that an existing biosensor for penicillin G detection is tedious in preparation process, complex in equipment and free of sufficiently low detection limit are overcome; and construction of an immunosensor for detecting penicillin G by adopting an unlabeled immunoelectrochemistry method is realized.

Description

technical field [0001] The invention belongs to the technical field of chemical detection and food safety, relates to nano functional material and sensor technology, in particular to a nano immune sensor for detection of penicillin G residue in dairy products and a preparation method thereof. Background technique [0002] Penicillin G (Penicillin G) belongs to the β-lactam antibiotics. Since its advent, it has been widely used because of its strong antibacterial effect, high curative effect and low toxicity. In animal husbandry, penicillin G is generally used as a routine drug for the prevention and treatment of dairy cow mastitis. Improper medication to dairy cows or failure to pay attention to the safe time of administration will result in antibiotic residues in milk. Long-term drinking of milk containing penicillin G residues by the general population will lead to drug resistance of some conditional pathogens in the body, and people who are allergic to penicillin G will ...

AI Technical Summary

Problems solved by technology

Microbiological assays are inexpensive but less sensitive

Physical and chemical detection methods specifically include colorimetry, thin layer chromatography (TLC), gas chromatography (GC) and high performance liquid chromatography (HPLC), etc., the above methods generally have high sensitivity and detection rate, but before the sample Complicated processing, long detection time, expensive instruments and equipment, and requires specialized technical personnel to operate

Enzyme-linked immunosorbent assay (ELISA) is the most widely used immunoassay method, which has the characteristics of high sensitivity and specificity. However, it also has the disadvantages of high cost and long detection time, so it is not suitable for large sample volume detection.

At present, most of the existing biosensors for antibiotic detection are based on enzymes or ion-selective electrodes and use indirect techniques to detect Penicillium G. The equipment and detection steps are complicated, and there is no low enough detection limit.

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