A method and primers for detecting index sequence contamination of a sequencing platform
An index sequence and sequencing platform technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of inability to clearly or accurately find index pollution sources, high detection costs, and no record of operational errors and other issues, to achieve the effect of easy promotion and use, simple and convenient operation, and high accuracy
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[0035]In this example, the sequencing library WHTRDRPEP00000082 is used as an example for testing, and the sequencing platform uses the illuminahiseq platform.
[0036] In the experimental design of WHTRDRPEP00000082, theoretically, only libraries with 5 indexes, 201, 202, 203, 204 and 205 should be included; The five indexes 204 and 205 also contain 2.17% of index 87. The results of data splitting are shown in Table 1, which means that one of the five sub-libraries has index 87 sequence contamination.
[0037] Table 1 Index split results of WHTRDRPEP00000082 library
[0038] sample index sequence 0mismatch 1mismatch total reads bases index87 ATGAGG 2.11% 0.06% 2.17% 4458446 802520280 201 ATCACG 17.04% 0.35% 17.40% 35795615 6443210700 11 202 CGATGT 20.42% 0.62% 21.04% 43293453 7792821540 11 203 TTAGGC 20.99% 0.68% 21.67% 44580695 8024525100 11 204 TGACCA 22.08% 0.60% 22.69% 46678174 8402071320...
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