A method and primers for detecting index sequence contamination of a sequencing platform

An index sequence and sequencing platform technology, which is applied in biochemical equipment and methods, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of inability to clearly or accurately find index pollution sources, high detection costs, and no record of operational errors and other issues, to achieve the effect of easy promotion and use, simple and convenient operation, and high accuracy
CN107164464BActive Publication Date: 2021-12-21WUHAN BGI CLINICAL LAB CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN BGI CLINICAL LAB CO LTD
Publication Date
2021-12-21

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Abstract

The application discloses a method and primers for detecting index sequence contamination of a sequencing platform. The method for detecting contamination of the index sequence of the sequencing platform of the present application includes using specific primers for the contamination index sequence to perform PCR amplification on all sub-libraries of the contaminated sequencing channel, and according to whether there is specific amplification in the PCR amplification result , to judge whether there is index sequence pollution in the corresponding sub-library, so as to detect the pollution source of the index sequence of the sequencing platform. The index sequence contamination detection method of the sequencing platform of the present application provides a brand-new solution and idea for the detection of index sequence contamination on the sequencing platform, which can quickly and specifically detect the source of index sequence contamination, and has low cost, high accuracy, and easy operation. Simple and convenient, easy to promote and use.
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Description

technical field

[0001] The present application relates to the field of nucleic acid sequencing, in particular to a method and primers for detecting index sequence contamination of a sequencing platform. Background technique

[0002] In the process of nucleic acid sequencing, especially for some genomes with relatively large sequencing volumes, it is usually necessary to construct multiple sub-libraries, each sub-library contains one or more index sequences (index in English), and the sequencing results are analyzed according to different index sequences. Split to get the complete genome sequence.

[0003] However, when the data is split, index pollution occasionally occurs, that is, additional index sequences that are not designed in each sub-library appear, which means that the sequencing platform has index sequence pollution. The contamination of index sequences directly affects the results of data splitting and the accuracy of sequencing results. Therefore, it is necess...

Claims

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