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Preparation method of breast cancer susceptibility gene immune sensor based on flaky strontium ferrite double amplification

An immunosensor and susceptibility gene technology, applied in the field of new functional materials and biosensing detection, to achieve the effect of promoting electron transfer, improving stability and short response time

Inactive Publication Date: 2016-02-03
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many clinical detection methods for tumor markers, but most of them require large-scale equipment and professional operators

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of an immunosensor for breast cancer susceptibility genes based on double amplification of flake strontium ferrite

[0032] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultra-pure water, adding 6 μL, 0.1 mg / mL nitrogen-doped graphene hydroxypropyl chitosan N-GSHPCS dispersion solution dropwise on the electrode surface, and drying at room temperature to form a film;

[0033] (2) Add 6 μL of antibody incubation product Ab-AgSrFe dropwise 12 o 19 Put the solution on the surface of the electrode, rinse it with ultrapure water, and dry it in a refrigerator at 4°C;

[0034] (3) Drop 6 μL of BSA solution with a mass fraction of 0.5% on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0035] (4) Drop 6 μL, 0.001~35ng / mL of different concentrations of breast cancer susceptibility gene antigen standard solution onto the surface o...

Embodiment 2

[0037] Example 2 Preparation of an immunosensor for breast cancer susceptibility genes based on double amplification of flake strontium ferrite

[0038] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water, adding 6 μL, 1 mg / mL nitrogen-doped graphene hydroxypropyl chitosan N-GSHPCS dispersion solution dropwise on the electrode surface, and drying at room temperature to form a film;

[0039] (2) Add 6 μL of antibody incubation product Ab-AgSrFe dropwise 12 o 19 Put the solution on the surface of the electrode, rinse it with ultrapure water, and dry it in a refrigerator at 4°C;

[0040] (3) Add 6 μL of BSA solution with a mass fraction of 2% on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0041] (4) Drop 6 μL, 0.001~35ng / mL of different concentrations of breast cancer susceptibility gene antigen standard solution onto the surface of the ...

Embodiment 3

[0043] Example 3Preparation of an immunosensor for breast cancer susceptibility genes based on double amplification of flake strontium ferrite

[0044] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water, adding 6 μL, 0.1~3mg / mL nitrogen-doped graphene hydroxypropyl chitosan N-GSHPCS dispersion on the electrode surface, and drying at room temperature to form a film;

[0045] (2) Add 6 μL of antibody incubation product Ab-AgSrFe dropwise 12 o 19 Put the solution on the surface of the electrode, rinse it with ultrapure water, and dry it in a refrigerator at 4°C;

[0046] (3) Add 6 μL of BSA solution with a mass fraction of 0.5-3.5% dropwise on the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4°C;

[0047] (4) Drop 6 μL, 0.001~35ng / mL of different concentrations of breast cancer susceptibility gene antigen standard solution onto the surface of the e...

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Abstract

The present invention relates to a preparation method of a breast cancer susceptibility gene immune sensor based on flaky strontium ferrite double amplification, and belongs to the technical fields of novel functional materials and biological sensors. According to the present invention, based on the characteristics of large effective specific surface area, excellent catalysis activity and the like of the flaky strontium ferrite, the sensitivity and stability of the immune sensor are significantly improved, and the important significance is provided for early diagnosis of breast cancer.

Description

technical field [0001] The invention relates to the preparation of a breast cancer susceptibility gene immunosensor based on double amplification of sheet-like strontium ferrite, using Ab-AgSrFe 12 o 19 At the same time, as a marker for detecting antibodies and capturing antibodies, an electrochemical immunosensor for detecting breast cancer susceptibility genes is prepared, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Cancer is a general term for a large class of malignant tumors. During the development of tumors, abnormal expression of tumor cells or certain substances produced by host cells in response to tumors can be used as tumor markers for clinical diagnosis and auxiliary diagnosis of certain tumors. Therefore, in clinical research, it is very important to develop a fast, simple and sensitive method for detecting tumor markers. [0003] The electrochemical immunosensor has the advantages o...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/532
CPCG01N33/57415G01N33/532
Inventor 任祥魏琴吴丹马洪敏庞雪辉闫涛张勇
Owner UNIV OF JINAN
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