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Method for preparing beta-ionone hapten, artificial antigen and antibody

A technology of ionone and artificial antigen, which is applied to the preparation of carboxylic acid esters, chemical instruments and methods, and the preparation of hydroxyl compounds, and can solve the technical requirements for the preparation and application of β-ionone haptens, expensive instruments, and β - Ionone is cumbersome to deal with and other problems, and achieves the effects of high purity and yield, easy control, high purity and yield

Pending Publication Date: 2022-06-28
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) At present, the methods for detecting β-ionone in natural plants and agricultural products at home and abroad are cumbersome in pretreatment, long in detection time, expensive in instruments and high in operating technical requirements. Screening Test Requirements
[0008] (2) At present, there is no related technology of β-ionone hapten, artificial antigen and antibody and its preparation method and application
[0009] (3) There is no method for rapid, sensitive and specific detection of β-ionone in the prior art

Method used

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  • Method for preparing beta-ionone hapten, artificial antigen and antibody
  • Method for preparing beta-ionone hapten, artificial antigen and antibody
  • Method for preparing beta-ionone hapten, artificial antigen and antibody

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preparation example Construction

[0047] The preparation method of β-ionone hapten provided in the embodiment of the present invention includes:

[0048] The carboxyl group is directly introduced through the aldehyde group of β-ionone, and the hydroxyl group is generated through the reduction reaction, and the spacer arm with the carboxyl group is obtained.

[0049] The structural formula of the β-ionone hapten preparation method provided in the embodiment of the present invention is as follows:

[0050]

[0051] like figure 1 As shown, the method for preparing β-ionone hapten provided in the embodiment of the present invention comprises the following steps:

[0052] S101, dissolving β-ionone in methanol, adding sodium borohydride in batches under ice-bath conditions, and reacting under ice-bath conditions for 1 hour;

[0053] S102, quench the reaction with saturated aqueous ammonium chloride solution, extract the organic phase with ethyl acetate, wash the organic phase with water, dry over anhydrous sodium...

Embodiment 1

[0078] The invention provides a hapten that can retain the characteristic structure of β-ionone to the greatest extent, and has a certain length of connecting arm and a preparation method of the hapten; artificial antigen and antibody are prepared from the hapten; Application of ionone immunodetection method.

[0079] (1) β-ionone hapten provided by the invention, it has following structural formula:

[0080]

[0081] The β-ionone hapten provided by the present invention is directly introduced into a carboxyl group through an aldehyde group on the β-ionone, so that it can be coupled with a carrier protein to obtain an artificial antigen for immunization. The β-ionone can retain all the chemical structures and characteristic groups of β-ionone to the greatest extent, and is coupled with the carrier protein at the same time. The highlight is the unique structure of β-ionone itself, which is used for subsequent animal immune response, preparation Strongly specific and highly ...

Embodiment 2

[0102] This embodiment provides a kind of beta-ionone hapten, and its structural formula is as follows:

[0103]

[0104] It can be seen that the hapten provided by the present invention directly introduces hydroxyl and carboxyl groups through the reduction of the aldehyde group on the β-ionone, retains the chemical structure and characteristics of the β-ionone to the greatest extent, and can be directly combined with the carrier. The protein coupling laid the foundation for the subsequent preparation of high-specificity and high-sensitivity β-ionone antibodies.

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Abstract

The invention belongs to the technical field of agricultural product and food quality detection, and discloses a method for preparing a beta-ionone hapten, an artificial antigen and an antibody, carboxyl groups are directly introduced through aldehyde groups of beta-ionone, hydroxyl groups are generated through reduction reaction, and spacer arms with carboxyl groups are obtained. The carboxyl group is directly introduced through the hydroxyl group on the beta-ionone, the chemical structure and characteristics of the beta-ionone are reserved to the maximum extent, the beta-ionone can be directly coupled with carrier protein, and a foundation is laid for subsequent preparation of the beta-ionone antibody with high specificity and high sensitivity. Meanwhile, based on an aldehyde group in a beta-ionone structure, a hydroxyl group is directly generated through a reduction reaction, a spacer arm with a carboxyl group is generated, raw materials are relatively common, reaction operation is simple, high-temperature and high-pressure conditions are not needed, control is easy, and the prepared beta-ionone hapten is relatively high in purity and yield.

Description

technical field [0001] The invention belongs to the technical field of agricultural product and food quality detection, in particular to a beta-ionone hapten, an artificial antigen and an antibody, a preparation method and an application. Background technique [0002] At present, β-ionone is pycnone, which belongs to terpenoids, mainly exists in raspberries, raspberries and other fruits and petals such as morning glory, and is also an important aroma component in tobacco. Beta-ionone has a low olfactory threshold and has a raspberry aroma at room temperature, which is softer and slightly more woody. When diluted, it has a violet flower and cedar-like aroma, and has a low raspberry-like fruity aroma, so it is a Key components of flavor components in tobacco. Tobacco companies in various countries try their best to reduce the content of tar in cigarette smoke, which leads to the obvious lack of aroma of cigarette smoke, which is difficult to meet the requirements of smoking c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/40C07C67/08C07K14/765C07K14/77C07K14/795C07K16/44G01N33/53G01N33/577
CPCC07C69/40C07C67/08C07C29/143C07K14/765C07K14/77C07K14/795C07K16/44G01N33/5308G01N33/577C07K2317/35C07K2317/92C07C2601/16C07C33/14
Inventor 方松刘雪孔凡玉郭永峰宁扬邱军宋德安
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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