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A preparation method of alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduced graphene

A technology of gold hybrid carbon black and alpha-fetoprotein, which is applied in chemiluminescence/bioluminescence, scientific instruments, and analysis through chemical reactions of materials, can solve the problems of radioactive pollution, long detection cycle, and low sensitivity. Achieve the effects of sensitive detection, low detection limit and enhanced sensitivity

Active Publication Date: 2017-04-19
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has the disadvantages of radioactive pollution, low sensitivity, long detection cycle, cumbersome steps, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 A kind of preparation method of alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduction graphene

[0037] (1) The glassy carbon electrode with a diameter of 4 mm is polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in turn, and rinsed with ultrapure water;

[0038] (2) 2 μL of alpha-fetoprotein antibody with a concentration of 1 mg / mL captured the substrate material gold hybrid carbon black intercalated reduced graphene solution on the electrode surface, and stored at 4 ℃;

[0039](3) Block non-specific active sites with 3 μL of bovine serum albumin solution with a mass fraction of 0.5%, incubate in a refrigerator at 4 °C for 1 h, and wash them clean;

[0040] (4) 2 μL of alpha-fetoprotein with a concentration of 0.0001 ~ 30 ng / mL of a series of different concentrations was used for the specific recognition of the capture antibody, incubated at room temperature for 1 h, and washed clean; ...

Embodiment 2

[0042] Embodiment 2 A kind of preparation method of alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduction graphene

[0043] (1) The glassy carbon electrode with a diameter of 4 mm is polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in turn, and rinsed with ultrapure water;

[0044] (2) 6 μL of alpha-fetoprotein antibody with a concentration of 2 mg / mL captured the substrate material gold hybrid carbon black intercalated reduced graphene solution on the electrode surface, and stored at 4 ℃;

[0045] (3) Block non-specific active sites with 6 μL of bovine serum albumin solution with a mass fraction of 1%, incubate in a refrigerator at 4 °C for 3 h, and wash them clean;

[0046] (4) 4 μL of alpha-fetoprotein with a concentration of 0.0001 ~ 30 ng / mL of a series of different concentrations was used for the specific recognition of the capture antibody, incubated at room temperature for 1 h, and washed clean;

...

Embodiment 3

[0048] Embodiment 3 A kind of preparation method of alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduction graphene

[0049] (1) The glassy carbon electrode with a diameter of 4 mm is polished with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in turn, and rinsed with ultrapure water;

[0050] (2) 10 μL of alpha-fetoprotein antibody with a concentration of 3 mg / mL was used to capture the substrate material gold hybrid carbon black intercalated reduced graphene solution on the electrode surface, and stored at 4 ℃;

[0051] (3) Block non-specific active sites with 9 μL of bovine serum albumin solution with a mass fraction of 1.5%, incubate in a refrigerator at 4 °C for 5 h, and wash clean;

[0052] (4) 6 μL of a series of different concentrations of alpha-fetoprotein with a concentration of 0.0001 ~ 30 ng / mL was used for the specific recognition of the capture antibody, incubated at room temperature for 1 h, and washed ...

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Abstract

The invention relates to a method for preparing an alpha fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduced graphene and belongs to the field of electrochemiluminescence sensors. Tris bipyridine ruthenium is taken as the electrochemiluminescence signal source, and the carrier content of an antibody is increased by means of the excellent biocompatibility and large specific surface area of gold hybrid carbon black intercalation reduced graphene. Nano-porous silver is taken as the second antibody marker, and the hepatoma marker alpha fetoprotein is detected based on the fact that electrochemiluminescence signal intensity changes as the concentration of tested substances changes.

Description

technical field [0001] The invention relates to a preparation method of an alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduction graphene. In particular, it relates to a gold hybrid carbon black intercalation reduced graphene as a base material, using the excellent biocompatibility and large specific surface area of ​​nanoporous silver to immobilize ruthenium terpyridine and a detection antibody, and prepare a detection alpha-fetoprotein. The electrochemiluminescence immunosensor belongs to the technical field of novel functional materials and biosensing detection. Background technique [0002] Primary liver cancer (Hepatocellular Carcinoma, HCC) is a very common malignant tumor with high incidence and poor prognosis. Alpha-fetoprotein (α-fetoprotein, αFP or AFP) is the only tumor marker widely used in the diagnosis of HCC, and is an important indicator in non-invasive diagnostic methods. Alpha-fetoprotein mainly comes ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
CPCG01N21/763
Inventor 魏琴朱文娟吴丹范大伟孙旭胡丽华马洪敏张勇庞雪辉杜斌
Owner UNIV OF JINAN
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