A preparation method of alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduced graphene

Abstract

The invention relates to a method for preparing an alpha fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduced graphene and belongs to the field of electrochemiluminescence sensors. Tris bipyridine ruthenium is taken as the electrochemiluminescence signal source, and the carrier content of an antibody is increased by means of the excellent biocompatibility and large specific surface area of gold hybrid carbon black intercalation reduced graphene. Nano-porous silver is taken as the second antibody marker, and the hepatoma marker alpha fetoprotein is detected based on the fact that electrochemiluminescence signal intensity changes as the concentration of tested substances changes.

Description

technical field

[0001] The invention relates to a preparation method of an alpha-fetoprotein electrochemiluminescence sensor based on gold hybrid carbon black intercalation reduction graphene. In particular, it relates to a gold hybrid carbon black intercalation reduced graphene as a base material, using the excellent biocompatibility and large specific surface area of ​​nanoporous silver to immobilize ruthenium terpyridine and a detection antibody, and prepare a detection alpha-fetoprotein. The electrochemiluminescence immunosensor belongs to the technical field of novel functional materials and biosensing detection. Background technique

[0002] Primary liver cancer (Hepatocellular Carcinoma, HCC) is a very common malignant tumor with high incidence and poor prognosis. Alpha-fetoprotein (α-fetoprotein, αFP or AFP) is the only tumor marker widely used in the diagnosis of HCC, and is an important indicator in non-invasive diagnostic methods. Alpha-fetoprotein mainly comes ...