36 results about "Membrane emulsification" patented technology

The invention discloses uniform-particle-size silk fibroin microspheres and a preparation method and application thereof.The preparation method comprises the following steps that a regenerated silk fibroin solution is taken and subjected to trypsin digestion to obtain modified silk fibroin sediment, and the modified silk fibroin sediment is dissolved in an aqueous solution of a beta-folding inhibitor to obtain a silk fibroin solution serving as a dispersed phase; emulsifying through an external pressure type membrane emulsifier to obtain an emulsion containing the silk fibroin emulsion droplets; adding a cross-linking agent into the emulsion to carry out cross-linking reaction, and then adding a crystallization inducer to induce crystallization to obtain primary silk fibroin microspheres; and performing vacuum freeze drying to obtain the silk fibroin microspheres. Silk fibroin is subjected to enzyme digestion modification, silk fibroin fragments with high beta-folding content are reserved, and high structural stability is achieved; the crystallinity of the microspheres is improved by adopting a membrane emulsification mode and combining a multiple crystallization induction method, and the silk fibroin microspheres with uniform particle size and smooth surfaces are obtained.

The invention provides a poly-L-lactic acid microsphere. The poly-L-lactic acid microsphere is obtained by performing membrane emulsification on a poly-L-lactic acid-polyethylene glycol copolymer synthesized by taking lactide and polyethylene glycol as raw materials. According to the L-polylactic acid microspheres disclosed by the invention, the uniformity, the stability and the biocompatibility of the microspheres are improved, and meanwhile, the synthesis of skin collagen is promoted.

The present application relates to a kind of surface patterning microspheres of uniform size and its preparation method and purposes, the preparation method includes: preparing W1 / O initial milk or oil phase O'; adding stabilizer and initiator synthesis W1 / O / W2 complex emulsion emulsion or O' / W2 single emulsion emulsion;Different surface patterning microspheres are prepared by rapid membrane emulsification method.The preparation method of the present application is simple in operation, mild condition, good batch repeatability and easy to enlarge.

The SPG membrane emulsifier comprises a shell, the upper end and the lower end of the shell are each provided with two counter bores, two membrane tubes are arranged in the shell, and the two ends of each membrane tube are located in the upper counter bores and the lower counter bores respectively; the upper end and the lower end of the shell are each connected with an interface piece. Two bosses are arranged at one end of the connector piece, stepped holes are formed in the bosses, the bosses are in butt joint in the counter bores, and the stepped holes wrap the ends of the membrane tube; and a sealing ring is arranged between the outer wall of the membrane tube and the inner wall of the counter bore. The shell is connected with a first flange, the interface piece is connected with a second flange, and the first flange and the second flange are connected through a knob bolt. And the sealing ring is tightly pressed by the boss. The outer wall of the shell is connected with an inlet pipe and an outlet pipe. The SPG membrane emulsifier with the limiting function aims to overcome the existing defects, and displacement of the membrane tube after installation is avoided.

The application discloses a high-drug-loading sustained-release microsphere and a preparation method and application thereof, and the raw materials of the microsphere comprise an active drug, a carrier material, a foaming pore-forming agent and a surfactant. In the microsphere preparation process, the SPG membrane emulsification method is adopted and the foaming pore-forming agent is added, so that the drug loading and the encapsulation efficiency of the microsphere can be effectively improved, the active drug can be released immediately and continuously and slowly, the injection amount and the injection volume of the microsphere are reduced, the release lag period of the drug-loaded microsphere is eliminated, and no oral supplement preparation needs to be combined in the early injection period, so that the patient compliance is improved.

The invention relates to the field of heavy oil emulsification, in particular to a device and a method for emulsifying and combusting biological heavy oil, which are characterized in that biological heavy oil is used as a raw material, water or urea water or other aqueous solution containing low-concentration ammonia is used as an emulsifying medium, and enters a shell pass of membrane emulsifying equipment under a certain temperature condition; heavy oil emulsion is generated under a certain pressure condition with biological heavy oil doped with a certain proportion of combustion-supporting liquid in a tube pass and enters a combustor to be combusted, so that the aims of sufficiently combusting the biological heavy oil and reducing the emission of NOx in tail gas are fulfilled. Biomass fuel such as biodiesel and biological methanol can be adopted as combustion-supporting liquid in the process. Along with the emulsion combustion process, the system dynamically adjusts the proportion of the combustion-supporting liquid in time. A set of biological heavy oil emulsion combustion process scheme is designed aiming at the contents, so that resource utilization of the biological heavy oil is realized, part of traditional fossil fuel is favorably replaced, and the contradiction between petroleum resource shortage and demand in China is solved.

The present disclosure relates to a travoprost sustained release biodegradable intracameral Implant comprising a biodegradable hydrogel and travoprost particles. The present disclosure also relates to travoprost particles optionally obtained by or obtainable by a process comprising a membrane emulsification step. The present disclosure also relates to a method of manufacturing travoprost particles, optionally wherein the method comprises a membrane emulsification step. The present disclosure also relates to a method of manufacturing a travoprost sustained release biodegradable intracameral implant. The present disclosure also relates to a method of treating an ocular disease, such as glaucoma or ocular hypertension, comprising administering the travoprost particles or inserting the intracameral implant of the present disclosure into an eye of a subject in need of treatment.

The present application relates to a kind of multiple membrane emulsification device and the method for being used as the preparation of narrow particle size distribution microspheres, device includes first emulsification kettle and second emulsification kettle;The first emulsification kettle and the second emulsification kettle bottom are respectively connected first check valve and second check valve, two the first check valve is connected to porous membrane tube jacket and the membrane tube outer cavity formed by porous membrane tube, and the direction of conduction direction points to membrane tube outer cavity, two second check valve is connected to the membrane tube inner cavity of porous membrane tube, and the direction of conduction direction points to emulsification kettle, the porous membrane tube is arranged in the porous membrane tube jacket;The first emulsification kettle and the second emulsification kettle top are communicated pressure conversion mechanism, and the pressure conversion mechanism is used to control first emulsification kettle and second emulsification kettle to carry out pressure conversion, to drive emulsion to realize multiple membrane emulsification. Utilize multiple passage conversion valve control emulsification kettle between pressure difference conversion, drive emulsion multiple membrane emulsification, obtain uniform particle size emulsion, preparation solidification polymer microsphere.

The invention provides a construction method and application of lactoferrin-phospholipid-based emulsion with a three-layer breast milk fat globule membrane imitating structure, and belongs to the technical field of dairy products, the construction method comprises the following steps: carrying out ultrasonic treatment on lactoferrin to obtain emulsion with a single-layer membrane structure; piling phospholipid and cholesterol through a microporous membrane to obtain a microporous membrane with a bimolecular membrane; and carrying out electrostatic self-assembly secondary covering on the emulsion with the single-layer membrane structure and a microporous membrane with a bimolecular membrane to obtain the lactoferrin-phospholipid-based emulsion with the three-layer breast milk fat globule membrane imitating structure. The lactoferrin-phospholipid-based emulsion with the three-layer breast milk fat globule membrane imitating structure is used for preparing infant formula emulsion. The invention establishes a novel breast milk fat globule structure imitating emulsion construction method, namely a microporous membrane stacking-electrostatic self-assembly secondary covering combined ultrasonic-premixed membrane emulsification technology, and the method is simple in equipment, simple and convenient to operate, green and pollution-free, energy-saving and consumption-reducing, free of temperature rise and shear force input, and suitable for heat-sensitive and mechanical force-sensitive materials.

The invention relates to the technical field of compounds, in particular to poly-L-lactic acid microspheres for injection and a preparation method of the poly-L-lactic acid microspheres. The microspheres prepared under the condition of high-speed shearing dispersion or mechanical stirring are uniform in particle size, narrow in particle size range and good in reproducibility. A polylactic acid raw material with proper molecular weight is screened, microspheres are manufactured through a membrane emulsification method, then the microspheres with the required particle size are screened through a screening mechanism, and the surfaces of the microspheres are smooth.

This invention discloses a uniform-size silk fibroin microsphere, its preparation method, and its application, comprising the following steps: taking a regenerated silk fibroin solution, digesting it with trypsin to obtain a modified silk fibroin precipitate, dissolving it in an aqueous solution of a β-sheet inhibitor to obtain a silk fibroin solution as the dispersed phase; emulsifying it using an external pressure membrane emulsifier to obtain an emulsion containing silk fibroin droplets; adding a cross-linking agent to the emulsion to carry out a cross-linking reaction, and then adding a crystallization inducing agent to induce crystallization to obtain primary silk fibroin microspheres; and freeze-drying under vacuum to obtain silk fibroin microspheres. This invention modifies silk fibroin by enzymatic digestion to retain silk fibroin fragments with high β-sheet content, exhibiting high structural stability; and uses membrane emulsification combined with a multiple crystallization induction method to improve the crystallinity of the microspheres, obtaining silk fibroin microspheres with uniform particle size and smooth surface.

The utility model relates to a device for low-carbon green manufacturing of glass by taking biological heavy oil as fuel power. Comprising a membrane emulsification system for producing a biological heavy oil W / O emulsion; the kiln system is used for realizing glass production by utilizing the biological heavy oil W / O emulsion; the medium-concentration product membrane separation system is used for capturing CO2 in flue gas produced in the glass production process at a time, part of produced medium-concentration CO2 product gas flows into the biological carbon sequestration system, and the other part of the produced medium-concentration CO2 product gas flows into the compensation type-high-concentration product membrane separation system after being pressurized; the biological carbon sequestration system is used for immobilizing and utilizing CO2 obtained by the medium-concentration product membrane separation system; and the compensation type-high-concentration product membrane separation system is used for carrying out secondary trapping and concentration on the medium-concentration CO2 product gas, and the produced high-concentration CO2 product gas is recycled to the membrane emulsification system.

The invention relates to a temperature change microcapsule preparation method, a temperature change microcapsule and an LED display module, the temperature change microcapsule preparation method comprises the following steps: preparing a core material dispersion liquid based on a core material component, preparing a shell material premix liquid based on a shell material component, combining the core material dispersion liquid and the shell material premix liquid to carry out film passing emulsification treatment to obtain emulsion particles, and carrying out staged UV curing treatment on the emulsion particles to obtain the temperature change microcapsule. The core-loaded microcapsule is obtained. And finally, depositing and curing a functional layer on the surface of the core-loaded microcapsule to obtain the temperature change microcapsule sequentially comprising the core material, the shell material and the functional layer from inside to outside. According to the scheme, the preparation process accuracy and application compatibility of the temperature change microcapsule can be effectively improved, and the problem that the temperature change microcapsule is poor in performance suitability and process compatibility in the application of an LED display screen is solved.

The invention relates to the technical field of daily chemicals, in particular to a bacteriostatic and acarus-killing laundry detergent and a preparation method thereof. According to the laundry detergent, a multi-stage microcapsule technology is adopted, and intelligent controlled release of sterilization components is achieved through a gradient silicon shell structure and a chitosan quaternary ammonium salt nano-micelle system; the preparation method comprises the following steps: preparing an enzyme preparation and a protective agent into an internal phase solution, and performing membrane emulsification on the internal phase solution and a sodium alginate shell phase solution to form microcapsules; a single silicon shell structure is constructed through modification of a silane coupling agent; then reacting with a composite nano-micelle dispersion liquid and water glass to form a composite microcapsule; and finally, compounding with a surfactant to obtain a finished product. The invention solves the technical problem that the bio-enzyme and the bactericide in the traditional laundry detergent are difficult to coexist, and the product has the characteristics of high-efficiency bacteriostasis and acarus killing, long-acting enzyme activity maintenance and low skin irritation, and can meet the diversified requirements from daily cleaning to professional sterilization.

The application discloses a TiO2@MXene ceramic membrane, a preparation method and application in milk preparation or demulsification, wherein the preparation method comprises the following steps: at least one layer of MXene nanosheet is prepared by using a wet chemical etching method; and a TiO2@MXene ceramic membrane with a 0D-2D structure is prepared on a ceramic support by using a high-temperature in-situ oxidation method or a self-assembly method; the TiO2@MXene ceramic membrane has a unique 0D-2D sandwich structure, the transmission path of a dispersed phase is enlarged, and the transmission resistance of a separated phase between layers is reduced, which is of great significance for a hydrophilic membrane to be used in membrane emulsification for preparing monodisperse W / O emulsion with controllable particle size; meanwhile, the TiO2@MXene ceramic membrane exhibits excellent oil stain resistance and a low flux decay rate in an oil-water separation process, and is also of great significance for realizing a high-stable oil-water separation process.

The invention provides a polylysine-containing composite embedded particle and a continuous membrane emulsification preparation method thereof, and the method comprises the following steps: dissolving epsilon-polylysine and galactose araban in water to obtain an internal water phase, mixing mixed MCT oil, phospholipid, polyglycerol fatty acid ester and hinokiol to obtain an external membrane oil phase, and carrying out continuous membrane emulsification on the external membrane oil phase to obtain the polylysine-containing composite embedded particle. Mixing the inner water phase and the outer membrane oil phase in a membrane emulsification mode to obtain W / O type primary emulsion; mixing polyglycerol fatty acid ester, cetostearyl alcohol, glycerol and water to obtain an out-of-membrane water phase, and mixing the W / O primary emulsion and the out-of-membrane water phase in a secondary membrane emulsification manner to obtain W / O / W multiple emulsion; the W / O / W multiple emulsion and maltodextrin are uniformly mixed in water and then spray-dried to obtain composite embedded particles containing polylysine, and stable encapsulation of epsilon-polylysine is realized by adopting an efficient and mild encapsulation technology.

The present invention discloses a device and method for realizing low-carbon green manufacturing of glass using bio-heavy oil as fuel power, belonging to the field of chemical engineering technology. The device includes a membrane emulsification system for producing bio-heavy oil W / O emulsion; a kiln system for realizing low-carbon preparation of glass using bio-heavy oil W / O emulsion; a medium-concentration product membrane separation system for capturing CO2 in flue gas and producing medium-concentration CO2 product gas; a biological carbon fixation system for producing high-value-added products by utilizing aquatic biological metabolism, combining CO2 photocatalytic mediating materials, and coordinating aquatic biological capacity expansion technology for CO2 fixation and utilization; a compensatory high-concentration product membrane separation system for secondary concentration of medium-concentration CO2 product gas. This application replaces or partially replaces fossil fuels with bio-heavy oil emulsion, reduces carbon emissions in the melting stage, and captures CO2 in flue tail gas, forming a multifunctional CO2 cascade utilization process.

Membrane emulsification can be performed using a membrane (16) having a microstructured surface. In some examples, a first side (50) of the membrane is contacted with a phase to be dispersed and the second side (52) of the membrane is contacted with a second liquid that is immiscible with the phase to be dispersed. The membrane includes microstructures formed on at least the second side of the membrane, e.g., to control the wettability of the membrane. The phase to be dispersed can be dispersed through a plurality of through holes (46) extending through the membrane to form droplets (48) and polymer beads formed from the droplets.

The invention discloses a self-repairing hydrophobic discontinuous air diffusion electrode, a preparation method and an application in electro-catalysis. The preparation method comprises the following steps: preparing a silicone oil-in-water emulsion by using a membrane emulsification method; preparing SiO2 silicone oil microspheres with a core-shell structure on the surface of the silicone oil emulsion by using a Stober method; preparing a self-repairing hydrophobic discontinuous air diffusion electrode by using a spraying method; the SiO2-coated silicone oil microsphere has a unique mesoporous structure, silicone oil can be periodically released from mesopores, the electrowetting resistance of an air diffusion electrode is improved, and the hydrophobic SiO2-coated silicone oil microsphere is of great significance in electrocatalytic efficient preparation of H2O2; and meanwhile, the method also has important significance for preparing an anti-electrowetting air diffusion electrode.

The invention discloses a device and method for large-scale preparation of liquid drops with uniform particle size, and belongs to the technical field of multi-channel membrane emulsification, and the device comprises a micro-channel emulsification membrane, a first chamber and a second chamber. The micro-channel structure is composed of an outlet, an inlet and a channel connecting the outlet and the inlet, wherein the outlet comprises a rectangular first groove and second grooves located in the two sides of the short edge of the outlet and communicated with the first groove. And etching a plurality of micro-channel structures on the thin film in an array manner to form the micro-channel emulsion film for preparing liquid drops with uniform particle sizes. And uniformly dispersing the curable dispersion phase in the continuous phase under the action of surface tension by using the emulsified film to form liquid drops with uniform particle size, then curing the liquid drops to form microspheres, and cleaning to obtain the microspheres with uniform particle size. The device provided by the invention can generate liquid drops and microspheres with more uniform particle sizes at a higher speed, the amplification process is easy, and the device is suitable for large-scale preparation of liquid drops and microspheres with uniform particle sizes.

The application discloses a kind of PHA microspheres, and its preparation method, preparation and application containing it.The preparation method of PHA microspheres includes the following steps: organic phase includes polyhydroxyalkanoate and organic solvent;Water phase includes surfactant and water;Organic phase and water phase are initially mixed, after membrane emulsification, volatile organic solvent, solidification, and PHA microspheres are prepared;Wherein, volatile includes first stage volatilization and second stage volatilization;The temperature T1 of first stage volatilization is 20~30 ℃;When 50~85 vol% of organic solvent is volatilized, second stage volatilization is carried out;The temperature T2 of second stage volatilization is 30~40 ℃;The temperature T3 of solidification is 40~50 ℃;T3>T2>T1, 5 ℃≤T2-T1≤20 ℃, 5 ℃≤T3-T2≤20 ℃.The surface of PHA microspheres of the application is smooth, roundness is high, particle size is uniform, particle size is concentrated in the range of 20~60 μm, use safety is high, microsphere fluidity is good, and it is easy to inject.

The invention belongs to the technical field of medicine preparation, and particularly relates to a perampanel sustained release microsphere, a pharmaceutical preparation and a preparation method based on membrane emulsification. The invention firstly provides a preparation method which comprises the following steps: (1) dissolving perampanel and a polylactic acid-glycolic acid copolymer in an organic solvent to prepare an oil phase; (2) preparing a water phase; (3) adding the oil phase into the water phase to prepare a pre-emulsion; (4) adding the pre-emulsion into a membrane emulsifier, and dispersing to obtain an emulsion; and (5) curing to obtain the drug microspheres. By strictly controlling the membrane emulsification process, the drug microspheres with uniform size and stable quality are obtained.

The present disclosure provides a process for preparing biopolymer particles, the process comprising emulsifying a dispersed phase film into a continuous phase, wherein the dispersed phase comprises a biopolymer in a solvent, and wherein the dispersed phase forms an emulsion of the biopolymer in the continuous phase through the film; and phase inversion with an antisolvent to form particles of the biopolymer; wherein prior to (b), the emulsion is cooled to a temperature T1. Also provided are biopolymer particles obtained from the process.

The invention relates to the technical field of microsphere preparation, and discloses a system for preparing liposome polymer microspheres by using a through-hole tubular filter membrane and a control method. The system for preparing the liposome polymer microspheres by using the through-hole tubular filter membrane comprises a membrane emulsification device, the membrane emulsification device comprises a plurality of tubular filter membranes; the plurality of tubular filter membranes are arranged in parallel; an outer flow channel is formed between every two adjacent tubular filter membranes; an inner flow channel is formed in the tubular filter membrane in the axial direction of the tubular filter membrane, a plurality of micropores are formed in a tube arm of the tubular filter membrane, and the outer flow channel is communicated with the inner flow channel through the micropores. By arranging a plurality of tubular filter membranes in parallel, a large amount of raw materials can be synchronously treated, the microsphere yield in unit time is remarkably improved, the bottleneck of low efficiency of a traditional single filter membrane is overcome, the device is suitable for industrial enlarged production, and the problems that in the prior art, the yield of a membrane emulsification device is limited, and the production efficiency is low in a large-scale emulsification scene are solved. A plurality of film emulsifying devices need to be arranged to participate in the emulsifying process at the same time, so that the cost of the emulsifying process is relatively high.

The invention relates to the technical field of dynamic membrane production equipment, and discloses a dynamic membrane emulsifying and homogenizing device which comprises a supporting sleeve and a limiting disc, the top end of the supporting sleeve is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a rotating shaft, and a mixing and defoaming mechanism is arranged on the outer side of the limiting disc. The mixing and bubble eliminating mechanism is used for rapidly mixing the raw materials and eliminating large bubbles generated during mixing, and a supporting, clamping and fixing mechanism is arranged on the outer wall of the supporting sleeve. The driving motor drives the rotating shaft to rotate, the limiting disc drives the inclined guide pipe to generate centrifugal suction force, upper-layer liquid is sucked in and guided to the bottom, meanwhile, the shaftless auger pushes bottom-layer liquid upwards, two layers of liquid collide and are mixed in the middle, and the I-shaped beating plate beats and scatters the liquid and pushes the liquid outwards; bubbles are extruded and broken, so that emulsification, homogenization and defoaming are realized.

This disclosure provides a method for preparing biopolymer particles. The method comprises: membrane emulsification of a dispersed phase in a continuous phase, where the dispersed phase comprises the biopolymer in a solvent, and where passage of the dispersed phase through the membrane forms an emulsion of the biopolymer in the continuous phase; and phase inversion with an antisolvent to form biopolymer particles; wherein, prior to step (b), the emulsion is cooled to temperature T1. Biopolymer particles obtained from the method are also provided.

The invention discloses a PHA (Polyhydroxyalkanoate) microsphere, a preparation method thereof, a preparation containing the PHA microsphere and application of the PHA microsphere. The preparation method of the PHA microspheres comprises the following steps: an organic phase comprises polyhydroxyalkanoate and an organic solvent; the water phase comprises a surfactant and water; the preparation method comprises the following steps: primarily mixing an organic phase and a water phase, volatilizing an organic solvent after membrane emulsification, and curing to prepare PHA microspheres; wherein the volatilization comprises first-stage volatilization and second-stage volatilization; the temperature T1 of the first-stage volatilization is 20-30 DEG C; after the organic solvent is volatilized by 50-85vol%, carrying out second-stage volatilization; the temperature T2 of the second-stage volatilization is 30-40 DEG C; the curing temperature T3 is 40-50 DEG C; t3 > T2 > T1, 5 DEG C < = T2-T1 < = 20 DEG C, and 5 DEG C < = T3-T2 < = 20 DEG C. The PHA microspheres are smooth in surface, high in roundness, uniform in particle size, high in use safety, good in flowability and easy to inject, and the particle size is concentrated in the range of 20-60 microns.

An apparatus for manufacturing polymer particles via membrane emulsification includes a tank (2) and a plurality of tubes (3), each tube including a membrane segment (26) mounted within a first tank chamber (14a) of the tank (2), the first tank chamber (14a) being configured to be connected to a source of a dispersed phase liquid, each tube including an inlet (22) configured to be connected to a source of a continuous phase, each membrane segment (26) including a plurality of pores (28) extending through a wall (16) of the membrane segment (26). Each tube (3) includes an outer tube segment (27) extending through a shell (11) of the tank surrounding the first tank chamber, wherein the plurality of pores or perforations are confined to the membrane segment (26) mounted within the first tank chamber (14a) of the tank (2), and wherein the outer tube segment (27) of each tube (3) is configured to be connected to an emulsion processing unit (30). Membrane tubes and a method for preparing biopolymer particles via membrane emulsification are also disclosed.

The invention provides a construction method of an emulsion with a three-layer breast milk-imitating fat globule membrane structure and application of the emulsion in preparation of a high-end infant formula emulsion, and belongs to the technical field of dairy products, the construction method comprises the following steps: carrying out membrane emulsification on lactoferrin and sphingosine to obtain an emulsion with a single-layer membrane structure; stacking the milk fat globule membrane phospholipid and cholesterol through a microporous membrane to obtain a microporous membrane with a bimolecular membrane; and carrying out electrostatic self-assembly secondary covering on the emulsion with the single-layer membrane structure and a microporous membrane with a bimolecular membrane to obtain the emulsion with the three-layer breast milk imitating fat globule membrane structure. The emulsion with the three-layer breast milk-imitating fat globule membrane structure is used for preparing high-end infant formula emulsion. The invention establishes a novel breast milk fat globule structure imitating emulsion construction method, which combines microporous membrane piling-electrostatic self-assembly secondary covering with direct membrane emulsification-premixed membrane emulsification technology, has the advantages of simple equipment, simplicity and convenience in operation, greenness, no pollution, energy conservation, consumption reduction, no temperature rise and high shear force input, and is suitable for heat-sensitive and mechanical force-sensitive materials.

The present invention provides a method of high-density cell culture comprising at least the following steps: S1: obtain hydrogel microcapsules through membrane emulsification; S2: encapsulate a selected number of cells per each obtained hydrogel microcapsule, and S3: culture the encapsulated cells in a cell culture medium. The invention further refers to Human Pluripotent Stem Cells (hPSCs) obtained through the above-described method, in particular for use in medicine, more in particular for use in a method of in vitro generation of mature megakaryocytes.