15 results about "Emulsion solvent evaporation" patented technology

The invention discloses a muscle-bone regeneration sustained-release preparation for targeting knee joint cartilage and a preparation process thereof, the preparation is composed of a cartilage targeting peptide modified PLGA-hyaluronic acid composite nanoparticle sustained-release carrier, an active regeneration component, a biocompatible matrix and an anti-inflammatory component, nanoparticles are prepared by a multiple emulsion solvent evaporation method, and targeting peptide is modified; the composite concentrated growth factors, stem cells and traditional Chinese medicine extracts have the functions of targeted enrichment, long-acting slow release, anti-inflammation and cartilage regeneration promotion, and can be used for knee joint cartilage defect repair.

The invention discloses a novel RNA (Ribonucleic Acid) nano vaccine for preventing and treating colorectal cancer as well as a preparation method and application of the novel RNA nano vaccine. According to the nano vaccine, biodegradable polylactic acid-glycolic acid copolymer nano particles are taken as a carrier, and full-tumor cell RNA is encapsulated by a multiple emulsion solvent evaporation method to form the nano vaccine RNA (at) PLGA; the nano vaccine can effectively protect RNA, shows an excellent tumor inhibition effect and good safety in an animal model, can reverse the immunosuppression state of a tumor microenvironment, and provides a new effective strategy for immunotherapy of colorectal cancer.

The invention discloses a nano-cellulose reinforced biomass-based high-strength furniture board and a preparation method thereof, and belongs to the technical field of furniture board preparation, and the method comprises the following steps: preparing ZnO nanowire functionalized CNF by using a sol-gel method and a hydrothermal method and inducing by using an orienting agent, then preparing ferulic acid and lignin-based microcapsules by using an emulsion solvent evaporation method, and finally preparing the nano-cellulose reinforced biomass-based high-strength furniture board. Then, mixing the ZnO nanowire functionalized CNF, ferulic acid and lignin-based microcapsules, hydroxypropyl methyl cellulose and the like at a high speed to obtain a compound, and finally, carrying out gradient temperature melt extrusion, and carrying out liquid nitrogen cooling molding at-40 DEG C; through the synergistic effect of vertical growth of the ZnO nanowires to enhance interface bonding, stable ultraviolet resistance of the microcapsules and optimized dispersion of the dispersing agent, the problems of mechanical attenuation / color fading caused by weak mechanical property and ultraviolet aging of a biomass-based plate and failure of a traditional anti-ultraviolet agent are solved, synergistic improvement of mechanical property and ultraviolet resistance is realized, and the mechanical property and the anti-ultraviolet property of the biomass-based plate are improved. And the requirements of stable load bearing and ultraviolet fading resistance of medium-and-high-end furniture are met.

The invention discloses a self-assembled spherical lignin particle (LN) / poly (butylene adipate-co-terephthalate) (PBAT) composite material and a preparation method thereof, and relates to the field of polymer composite materials. The preparation method comprises the following steps: preparing a ternary green deep eutectic solvent (DES) from choline chloride / 1, 4-butanediol / aluminum chloride according to a molar ratio of 25: 50: 1, extracting lignin from bamboo powder, and preparing self-assembled spherical lignin particles LN through an ethyl acetate emulsion solvent evaporation method; and carrying out melt blending on LN and PBAT according to a ratio of 5-20wt%: 95-80wt%, and carrying out panel vulcanization shaping to obtain the composite material. Lysine diisocyanate (LDI), comb-shaped SG or cage-shaped POSS can also be added as a reactive compatibilizer. The method is green and environment-friendly, the elasticity modulus of the prepared composite material can reach up to 125 MPa, the tensile strength can reach up to 30 MPa, and the composite material is excellent in ultraviolet resistance and aging resistance and suitable for the fields of green packaging and intelligent agricultural films.

The invention discloses a bionic scaffold for treating osteoporotic bone defect, and relates to a bionic material, the scaffold is composed of three parts, namely melatonin-loaded PLGA sustained release microspheres, a silk fibroin / nano-hydroxyapatite (SF / n-HA) composite scaffold and methacrylic anhydride gelatin (GelMA) hydrogel wrapping bone marrow mesenchymal stem cells. The preparation method comprises the following steps: firstly, preparing PLGA sustained-release microspheres by an emulsion solvent evaporation method, and encapsulating melatonin in a PLGA matrix; secondly, mixing SF and n-HA according to a specific proportion, and freeze-drying to form a porous scaffold; then, the microspheres are compounded in pores of the stent through vacuum adsorption; and finally, performing negative pressure perfusion on the stem cell-containing GelMA hydrogel, and performing ultraviolet light crosslinking and curing on the surface of the composite scaffold. According to the scheme, the synergistic effect of drug sustained release, bone conduction and stem cell osteogenesis activity is achieved through layered structure design, and the problems that in the prior art, drug release is unstable, cell distribution is uneven, and stent degradation and bone regeneration are not matched are solved.

The invention belongs to the technical field of biological medicines, and particularly relates to a bladder cancer targeted nano-drug and a preparation method thereof. The drug is constructed by taking a chemotherapeutic drug gemcitabine-loaded poly (lactic-co-glycolic acid) nanoparticle as a core and assembling two functional components, namely a gadolinium-doped zinc-aluminum layered double hydroxide folic acid conjugate and a bismuth-tellurium alloy nano-cluster hyaluronic acid compound, on the surface of the nanoparticle. During preparation, the drug-loaded nanoparticles are prepared by adopting a multiple emulsion solvent evaporation method, and then the two functional compounds are co-assembled on the surfaces of the drug-loaded nanoparticles by utilizing electrostatic interaction to form the multifunctional hybrid shell layer. The nano-drug integrates multiple functions such as active targeting, microenvironment response drug release, multi-mode imaging, chemotherapy and chemical kinetics combined treatment and radiotherapy sensitization, enrichment and permeation of the drug at a bladder tumor part can be remarkably improved, efficient synergistic treatment is achieved, system toxicity is reduced, and the nano-drug is particularly suitable for perfusion treatment in the bladder.

The present application relates to a ginsenoside Rg3 drug delivery system, and a preparation method and application thereof. White yam polysaccharides (BSPs) are extracted from white yam by water extraction and alcohol precipitation, and then subjected to defatting, decolorization, deproteinization and chromatographic purification. A small hydrophobic molecule SA is introduced to modify the structure of BSPs, and a dialysis method is used to prepare BSPs-SA polymer micelles, and an amphiphilic copolymer BSPs-SA is obtained as a carrier material. An emulsion solvent evaporation method is used to prepare G-Rg3@BSPs-SA polymer micelles, and the freeze-dried powder is loaded into a colon-soluble capsule shell to prepare a G-Rg3@BSPs-SA colon-soluble capsule with double targeting. The in vitro drug release behavior and preliminary stability of the capsule are evaluated. After oral administration of the delivery system, no drug is released in the upper gastrointestinal tract, and after entering the colon, the capsule shell is degraded in the specific pH environment of the colon to release G-Rg3@BSPs-SA polymer micelles. Through the dual targeting function on tumor cells and M2-TAMs, the delivery system can achieve multi-link regulation of "inhibiting tumor cell growth and regulating immune microenvironment" of colon cancer, and effectively inhibit the occurrence and development of colon cancer.

The application relates to the field of biomaterials and regenerative medicine, in particular to a preparation method and application of fibronectin and collagen protein-loaded PLGA nanoparticles, wherein the preparation method adopts a complex emulsion solvent evaporation method, a buffer solution containing fibronectin, collagen protein and trehalose is used as an inner water phase, a dichloromethane solution of PLGA is used as an oil phase, and a polyvinyl alcohol and trehalose-containing aqueous solution is used as an outer water phase; and the nanoparticles are prepared through emulsification, high-pressure homogenization, dialysis and freeze-drying. The method has the beneficial effects of stable process and effective protection of protein activity. The obtained nanoparticles have uniform particle sizes (100-200 nm), and can realize synergistic controllable release of double proteins. The product organically combines the physical filling support function of PLGA and the biological repair function of active factors, realizes the integration of "filling-regeneration", can significantly reduce the risk of inflammation, and can be safely applied to soft tissue filling, wrinkle improvement and skin wound repair in the field of medical cosmetology.

The present invention relates to the technical field of functional composite materials, and disclosed are a preparation method for polylactic acid porous microspheres, and a use thereof in stem cell seeding. The method comprises the following steps: (1) dissolving chitosan in an acetic acid aqueous solution, adding bioactive glass nanoparticles, and performing ultrasonic treatment, to obtain an aqueous phase solution; (2) dissolving PLGA or PLA in dichloromethane and adding an emulsifier, to obtain an oil phase solution; (3) adding the aqueous phase solution dropwise into the oil phase solution, to obtain a water-in-oil initial emulsion; (4) adding the water-in-oil primary emulsion dropwise into a PVA aqueous solution, to obtain a double emulsion; (5) stirring the double emulsion, then performing centrifugation and drying, followed by crosslinking treatment in a glutaraldehyde solution, to obtain porous microspheres. The present invention prepares porous microspheres by means of a double emulsion solvent evaporation method, resulting in more uniform pore size distribution and higher bioactivity, which can thus act as an excellent carrier for stem cell seeding, and support high-efficiency cell adhesion and proliferation.

The present invention belongs to the technical field of pharmaceuticals, and relates to a carvedilol phosphate resin sustained-release microcapsule and preparation and use thereof. The carvedilol phosphate resin sustained-release microcapsule comprises a carvedilol phosphate resin and a capsule material. The capsule material is one or two of ethyl cellulose and acrylic resin, and the amount is 5-15% of the weight of the carvedilol phosphate resin. The carvedilol phosphate resin is prepared from carvedilol phosphate and an ion exchange resin by means of a static method or a dynamic method. The mass ratio of carvedilol phosphate to the ion exchange resin is 2:1 to 1:4. In the present invention, the ion exchange resin is used as a drug carrier to prepare the carvedilol phosphate resin microcapsule. The sustained-release effect of carvedilol phosphate is successfully achieved in vitro by means of ion exchange and emulsion solvent evaporation coating techniques. The prepared carvedilol phosphate resin microcapsule has an in vitro release rate of 30-50% in a buffer solution of 0.15 mol / L CH3COONa + 1 mol / L CH3COOH at 24 h. The ion exchange reaction between carvedilol phosphate and a cationic resin conforms to a first-order kinetic model.

The invention discloses a water-based thermoplastic sizing agent doped with a nano material as well as a preparation method and application of the water-based thermoplastic sizing agent, and belongs to the technical field of composite materials. According to the sizing agent, a bisphenol compound-diphenolic acid-dihalogen benzophenone / sulfone copolymer is used as matrix resin, a carboxyl-containing polyaryletherketone / sulfone copolymer is synthesized through SN2 nucleophilic condensation polymerization, the carboxyl-containing polyaryletherketone / sulfone copolymer is prepared into the water-based sizing agent by adopting an emulsion / solvent evaporation method, and nano materials such as graphene oxide and multi-walled carbon nanotubes are further doped. The sizing agent takes water as a dispersion medium, is green and environment-friendly, has excellent heat resistance, has good compatibility with phenolphthalein polyether ketone and other high-performance thermoplastic resins, and can significantly improve the interface bonding strength, interlaminar shear strength, bending strength and monofilament tensile strength of the carbon fiber composite material. The preparation process is simple and controllable, and is suitable for preparing high-performance carbon fiber composite materials in the fields of aerospace, mechano-electronics and the like.

The invention relates to the field of medicines, in particular to a brexpiprazole sustained-release microsphere and a preparation method thereof. The brexpiprazole sustained-release microsphere is prepared by adopting an emulsion solvent evaporation method, and the pH value of an external water phase is adjusted to be alkaline. The brexpiprazole sustained-release microsphere disclosed by the invention can realize high drug loading capacity and good balling property.

This invention relates to a method for preparing and applying a nanoprobe for multimodal imaging and synergistic therapy, belonging to the field of bioprobe technology. The method includes: loading the acoustic sensitizer MnTTP and the hypoxia-activating drug TPZ onto PLGA via an emulsion-solvent evaporation method, modifying it to an aqueous phase using PVA, using dopamine hydrochloride to increase biocompatibility, obtaining the nanoprobe TMP@D, and then... (The sentence is incomplete in the original text). 131 I-labeling on TMP@D yields radionuclide-loaded nanoprobes. 131 I-TMP@D. 131 I-TMP@D passively targets and enters tumor cells, enabling synergistic treatment of malignant hypoxic tumors by combining MR and SPECT multimodal imaging with radionuclide therapy, sonodynamic therapy, and hypoxia-activated chemotherapy.

The invention belongs to the technical field of biological medicine and nanometer, and particularly relates to a nanometer medicine loaded with astragalus polysaccharide and a preparation method and application thereof.According to the nanometer medicine, biodegradable polylactic acid-glycolic acid copolymer serves as a carrier, astragalus polysaccharide is encapsulated through a multiple emulsion solvent evaporation method, and the nanometer medicine is prepared. The obtained nano-particles can effectively realize efficient delivery of astragalus polysaccharide, in-vitro experiments prove that the nano-drug can inhibit tumor cell migration and invasion, in a colorectal cancer tumor-bearing mouse model, the nano-drug can significantly inhibit tumor growth and activate adaptive immune response, and the nano-drug is simple and convenient in preparation method, good in safety and suitable for industrial production. And a new effective strategy is provided for immunotherapy of colorectal cancer.

The invention discloses a double-targeting and double-response procyanidine carrying system as well as a preparation method and application thereof, and belongs to the technical field of nanotechnology. The preparation method comprises the following steps: activating (5-carboxyl amyl) triphenyl phosphorus bromide, adding proanthocyanidins to carry out esterification reaction, stirring, dialyzing, and freeze-drying to obtain a proanthocyanidins-(5-carboxyl amyl) triphenyl phosphorus bromide conjugate; the preparation method comprises the following steps: activating lactobionic acid LA, then mixing with hyaluronic acid HA to obtain an LH conjugate, and coupling 3-aminophenylboronic acid with the LH through an amide reaction to obtain an LHA conjugate; the procyanidine-(5-carboxyl amyl) triphenyl phosphorus bromide conjugate is subjected to a double-emulsion solvent evaporation method, and procyanidine-(5-carboxyl amyl) triphenyl phosphorus bromide nanoparticles are obtained; the preparation method comprises the following steps: modifying sodium alginate to obtain amphiphilic sodium alginate, and carrying out solvent evaporation on the amphiphilic sodium alginate and nanoparticles to obtain the procyanidine carrying system with double targeting and double response. The system can effectively realize the release of procyanidine under the stimulation of pH and active oxygen, and realizes the aggregation of procyanidine in liver and mitochondria.