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Novel injection self-condensing composite artificial bone carrying rhBMP_2 micro spheres

An artificial bone and a new technology, applied in the field of preparation of a new type of injectable self-solidifying composite artificial bone, can solve the problems of lack of mechanical strength and less extraction.

Pending Publication Date: 2019-05-21
PLA NO 5 HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Because inorganic materials are not easy to be absorbed, especially inorganic materials that have been burned at high temperature, the interface with the surrounding tissue will exist for a long time after implantation; although organic materials have better bone-inducing performance, they lack sufficient mechanical strength in the early stage of implantation, and The amount of extraction is less; therefore, the research on artificial bone tends to develop towards composite materials, even if the material contains both organic and inorganic components, so that it has both advantages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of modified CPC: Add 0.1MM sodium hydroxide solution to neutrality in 0.04M sodium hydrogen phosphate solution, collect the precipitate, add dropwise 1-(3-dimethylaminopropyl)-3-ethylcarbodia Physiological saline of amine hydrochloride, the amount added to 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 2% by mass of the calcium phosphate bone cement, stirred evenly, dried, washed After 3 times, dry grind.

[0027] 20% PLGA / dichloromethane 1ml plus 0.16% rhBMP_2 solution 100μl (containing 0.1% bovine serum albumin) emulsification 1min, add 1ml 1% PAV emulsification 1min, add 1% PAV 100ml and 0.5% isopropanol 100ml, at 300r / min Stir for 4 hours, let stand for 30 minutes, freeze-dry after washing, and store at -20°C;

[0028] Composite microspheres carrying RHBMP_2 and modified calcium phosphate bone cement powder were mixed at a volume ratio of 60:40, and then a solution containing 1% collagen was added until the collagen accounted for 7.5% of...

Embodiment 2

[0030] Preparation of modified CPC: Add 0.1MM sodium hydroxide solution to neutrality in 0.04M sodium hydrogen phosphate solution, collect the precipitate, add dropwise 1-(3-dimethylaminopropyl)-3-ethylcarbodia Physiological saline of amine hydrochloride, the amount added to 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 1% by mass of calcium phosphate bone cement, stirred evenly, dried, washed After 3 times, dry grind.

[0031] 20% PLGA / dichloromethane 1ml plus 0.16% rhBMP_2 solution 100μl (containing 0.1% bovine serum albumin) emulsification 1min, add 1ml 1% PAV emulsification 1min, add 1% PAV 100ml and 0.5% isopropanol 100ml, at 300r / min Stir for 4 hours, let stand for 30 minutes, freeze-dry after washing, and store at -20°C;

[0032] Composite microspheres carrying RHBMP_2 and modified calcium phosphate bone cement powder were mixed at a volume ratio of 50:50, and a solution containing 1% collagen was added until the collagen was 5% of the total mass and n...

Embodiment 3

[0034] Preparation of modified CPC: Add 0.1MM sodium hydroxide solution to neutrality in 0.04M sodium hydrogen phosphate solution, collect the precipitate, add dropwise 1-(3-dimethylaminopropyl)-3-ethylcarbodia Physiological saline of amine hydrochloride, the amount added to 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 3% by mass of calcium phosphate bone cement, stirred evenly, dried, washed After 1-3 times, dry grind.

[0035] 20% PLGA / dichloromethane 1ml plus 0.16% rhBMP_2 solution 100μl (containing 0.1% bovine serum albumin) emulsification 1min, add 1ml 1% PAV emulsification 1min, add 1% PAV 100ml and 0.5% isopropanol 100ml, at 300r / min Stir for 4 hours, let stand for 30 minutes, freeze-dry after washing, and store at -20°C;

[0036] Composite microspheres carrying RHBMP_2 and modified calcium phosphate bone cement powder were mixed at a volume ratio of 70:30, and then a solution containing 1% collagen was added until the collagen was 10% of the total ma...

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PUM

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Abstract

The invention provides a novel injection self-condensing composite artificial bone carrying rhBMP_2 micro spheres. The artificial bone comprises raw materials of composite micro spheres carrying rhBMP_2, modified calcium phosphate bone cement CPC, collagen and nano bacterial cellulose, wherein the rhBMP_2 composite micro spheres are prepared by PLGA, rhBMP_2 and PAV by adopting a multiple emulsion-solvent evaporation technology; the modified calcium phosphate bone cement is prepared by the mixing of hydroxyl poly calcium sodium phosphate powder and physiological saline with dissolved carbodimide. According to the composite micro spheres carrying the rhBMP_2, the volume ratio of the modified calcium phosphate bone cement is 50-70:30-50, the collagen adding amount is 5-10% of the total mass,the adding amount of the nano bacterial cellulose is 2-6% of the total mass. The composite artificial bone has good bone forming osteoinductive performance, and the mechanical strength is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing a novel injectable self-solidifying composite artificial bone carrying rhBMP_2 microspheres. Background technique [0002] In the field of orthopedics, bone defects due to severe trauma, bone tumors, osteomyelitis and other reasons are very common. Currently commonly used bone repair materials include autogenous bone and metal prosthesis. Autogenous bone increases the trauma and pain of patients; metal prosthesis has problems such as loosening and fracture. Therefore, artificial bone substitute material transplantation to repair bone defects has become a medical focus. Artificial bone refers to artificial biomaterials that can replace human bone or repair bone tissue defects. When it is time to replace a joint or treat a broken bone, the ideal way is to achieve bone self-repair through tissue regeneration function. However, in many cases, human bone cannot ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/24A61L27/50A61L27/54A61L27/58
Inventor 费正奇
Owner PLA NO 5 HOSPITAL
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