Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof

A composite scaffold and bone repair technology, applied in the field of biomedical engineering and biomedical materials, can solve the problems of cytotoxicity, small porosity, and inability to make larger pore diameters, and achieve simple and fast preparation process, improve mechanical properties, and good Effect of Drug Loading and Release Properties

Active Publication Date: 2015-11-11
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method can only prepare scaffolds with a pore size of less than 200 μm, and has the disadvantages of small porosity, difficult control of the geometry of the scaffold, and poor connectivity between pores.
For example, the pore size of the scaffold prepared by the phase separation method is relatively small; the gas foaming method cannot a...

Method used

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  • Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof
  • Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof
  • Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Prepare a PLGA cube support matrix with a regular three-dimensional macroporous structure:

[0046] Use the BioplotterRP software to process the STL format data of the cube model with a length of 10 mm, a width of 10 mm, and a height of 2 mm. Add 2 g PLGA to the stainless steel barrel, select a needle of 0.3 mm, open the Visual Machines software, set the printing temperature to 150 ° C, and the platform temperature to 25°C, the extrusion pressure is 1.5bar, the extrusion speed is 3mm / s, the internal structure is set to alternate between 0° and 90° of the nozzle, the layer thickness is 0.24mm, the hole diameter is 1.2mm, and then the material is heated to the specified temperature After 30 minutes of heat preservation, start the 3D-Bioplotter to print the three-dimensional structure model layer by layer, forming a regular three-dimensional macroporous structure in the CAD model. Figure 4 ;

[0047] (2) Preparation of PLGA / CS / HMS composite microspheres loaded with r...

Embodiment 2

[0052] (1) Preparation of a PLGA cylindrical scaffold matrix with a regular three-dimensional macroporous structure:

[0053] Use the BioplotterRP software to process the STL format data of the cylinder model with a diameter of 10mm and a height of 2mm in layers, add 2gPLGA into the stainless steel barrel, select a needle of 0.2mm, open the VisualMachines software, set the printing temperature to 150°C, and the platform temperature to 25°C, the extrusion pressure is 3.0bar, the extrusion speed is 2mm / s, the internal structure is set to alternate nozzles at 0°, 45°, 90°, and 145°, the layer thickness is 0.16mm, and the hole diameter is 1.0mm, and then Heat the material to the specified temperature and keep it warm for 30 minutes, start 3D-Bioplotter to print the three-dimensional structure model layer by layer, and form a PLGA cylindrical support matrix with a regular three-dimensional macroporous structure in the CAD model;

[0054] (2) Preparation of PLGA / CS / HMS composite mic...

Embodiment 3

[0059] (1) Preparation of a polycaprolactone (PCL) cube scaffold matrix with a regular three-dimensional macroporous structure:

[0060] Use the BioplotterRP software to perform hierarchical processing on the STL format data of the cube model with a length of 10mm, a width of 10mm, and a height of 2mm. Add 2gPCL to the stainless steel barrel, select a needle of 0.4mm, open the VisualMachines software, set the printing temperature to 160°C, and the platform temperature to 25°C, the extrusion pressure is 1.2bar, the extrusion speed is 3mm / s, the internal structure is set to alternate between 0° and 90° of the nozzle, the layer thickness is 0.32mm, the hole diameter is 1.2mm, and then the material is heated to the specified temperature After 30 minutes of heat preservation, start the 3D-Bioplotter to print the three-dimensional structure model layer by layer, forming the regular three-dimensional large-pore structure PCL cube support matrix in the CAD model;

[0061] (2) Preparat...

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Abstract

The invention discloses a bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and a preparation method thereof. The scaffold is formed by compounding a matrix with a 3D macroporous structure and a drug carrying microsphere. The preparation method comprises the following steps: printing a scaffold matrix with a regular 3D macroporous structure through 3D-Bioplotter; preparing a drug carrying microsphere compounding hexagonal mesoporous silica (HMS), calcium silicate (CS) powder and PLGA through an emulsion solvent evaporation method; finally, fixing the compound microsphere into the matrix through low-temperature sintering so as to obtain the bone repair porous compound scaffold based on 3D-Bioplotter printing technology. According to the invention, the 3D printed porous scaffold and the PLGA/HMS/CS compound microsphere with drug sustained release and bone repair effects are combined, so that the scaffold has a macroporous structure, has good drug carrying and drug release properties and osteogenic differentiation capability and can be used for effectively promoting the repair and reconstruction of bone tissues.

Description

technical field [0001] The invention relates to the technical fields of biomedical engineering and biomedical materials, in particular to a porous composite scaffold for bone repair based on 3D-Bioplotter printing technology and a preparation method thereof. Background technique [0002] Bone is an important organ of the human body, responsible for functions such as support, movement, protection, hematopoiesis, mineral storage and metabolism. Clinically, large bone defects and osteoporosis caused by trauma, infection, tumor, and congenital dysplasia exceed the self-repair ability of bone, and bone repair treatment is required. Traditional bone defect treatment methods mainly include autologous bone transplantation and allogeneic bone transplantation. Due to the limited source of autologous bone transplantation and secondary surgery, it brings more pain to patients; allogeneic bone transplantation also has immune rejection and carries viruses. And the risk of bacteria, so th...

Claims

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Application Information

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IPC IPC(8): A61L27/02A61L27/18A61L27/54A61L27/56
CPCA61L27/02A61L27/18A61L27/54A61L27/56
Inventor 魏坤胡露
Owner SOUTH CHINA UNIV OF TECH
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