Porous composite scaffold for bone repair based on 3d-bioplotter printing technology and its preparation method

A composite scaffold and bone repair technology, applied in the field of biomedical engineering and biomedical materials, can solve the problems of inability to make large pore size, cytotoxicity, small porosity, etc., achieve good drug loading and drug release performance, and simple preparation process Quick, improve the effect of mechanical properties

Active Publication Date: 2018-04-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method can only prepare scaffolds with a pore size of less than 200 μm, and has the disadvantages of small porosity, difficult control of the geometry of the scaffold, and poor connectivity between pores.
For example, the pore size of the scaffold prepared by the phase separation method is relatively small; the gas foaming method cannot accurately control the porosity, the shape of the scaffold, and cannot make a larger pore size; the porogen content of the particle leaching method will seriously affect the mechanical strength of the scaffold. , and the residue of the porogen will lead to cytotoxicity; the mechanical properties of the scaffold prepared by electrospinning technology are low, etc.

Method used

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  • Porous composite scaffold for bone repair based on 3d-bioplotter printing technology and its preparation method
  • Porous composite scaffold for bone repair based on 3d-bioplotter printing technology and its preparation method
  • Porous composite scaffold for bone repair based on 3d-bioplotter printing technology and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Prepare a PLGA cube support matrix with a regular three-dimensional macroporous structure:

[0046] Use the Bioplotter RP software to perform hierarchical processing on the STL format data of the cube model with a length of 10mm, a width of 10mm, and a height of 2mm, add 2gPLGA to the stainless steel barrel, select a needle of 0.3mm, open the VisualMachines software, set the printing temperature to 150°C, and the platform temperature The temperature is 25°C, the extrusion pressure is 1.5bar, the extrusion speed is 3mm / s, the internal structure is set to alternate between 0° and 90° of the nozzle, the layer thickness is 0.24mm, and the hole diameter is 1.2mm, and then the material is heated to the specified After keeping the temperature for 30 minutes, start the 3D-Bioplotter to print the three-dimensional structure model layer by layer, forming a regular three-dimensional macroporous structure in the CAD model. The PLGA cube support matrix, such as Figure 4 ;

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Embodiment 2

[0052] (1) Preparation of a PLGA cylindrical scaffold matrix with a regular three-dimensional macroporous structure:

[0053] Use the Bioplotter RP software to process the STL format data of the cylinder model with a diameter of 10mm and a height of 2mm in layers, add 2gPLGA into the stainless steel barrel, select a 0.2mm needle, open the VisualMachines software, set the printing temperature to 150°C, and the platform temperature The temperature is 25°C, the extrusion pressure is 3.0bar, and the extrusion speed is 2mm / s. The internal structure is set to alternate nozzles at 0°, 45°, 90°, and 145°, the layer thickness is 0.16mm, and the hole diameter is 1.0mm. Then heat the material to the specified temperature and keep it warm for 30 minutes, start the 3D-Bioplotter to print the three-dimensional structure model layer by layer, and form a PLGA cylindrical support matrix with a regular three-dimensional macroporous structure in the CAD model;

[0054] (2) Preparation of PLGA / CS...

Embodiment 3

[0059] (1) Preparation of a polycaprolactone (PCL) cube scaffold matrix with a regular three-dimensional macroporous structure:

[0060] Use the Bioplotter RP software to process the STL format data of the cube model with a length of 10mm, a width of 10mm, and a height of 2mm. The temperature is 25°C, the extrusion pressure is 1.2bar, the extrusion speed is 3mm / s, the internal structure is set to alternate between 0° and 90° of the nozzle, the layer thickness is 0.32mm, and the hole diameter is 1.2mm, and then the material is heated to the specified After keeping the temperature for 30 minutes, start the 3D-Bioplotter to print the three-dimensional structure model layer by layer, forming the regular three-dimensional macroporous structure of the PCL cube support matrix in the CAD model;

[0061] (2) Preparation of PLGA / CS / HMS composite microspheres loaded with isoniazid:

[0062] Mix 30mg of isoniazid and 1g of hexagonal mesoporous silicon with an average pore diameter of 2.5...

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PUM

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Abstract

A bone repair porous compound stent based on 3D-Bioplotter printing technology and a preparation method therefor. The stent is formed by compounding a matrix provided with a 3D macroporous structure and a drug carrying microsphere. The preparation method comprises the following steps: printing a stent matrix having a regular 3D macroporous structure by means of 3D-Bioplotter; preparing a drug carrying microsphere compounding hexagonal mesoporous silica (HMS), calcium silicate (CS) powder and PLGA by means of an emulsion solvent volatilization method; finally, fixing the compound microsphere into the matrix by means of low-temperature sintering, so as to obtain the bone repair porous compound stent based on 3D-Bioplotter printing technology.

Description

technical field [0001] The invention relates to the technical fields of biomedical engineering and biomedical materials, in particular to a porous composite scaffold for bone repair based on 3D-Bioplotter printing technology and a preparation method thereof. Background technique [0002] Bone is an important organ of the human body, responsible for functions such as support, movement, protection, hematopoiesis, mineral storage and metabolism. Clinically, large bone defects and osteoporosis caused by trauma, infection, tumor, and congenital dysplasia exceed the self-repair ability of bone, and bone repair treatment is required. Traditional bone defect treatment methods mainly include autologous bone transplantation and allogeneic bone transplantation. Due to the limited source of autologous bone transplantation and secondary surgery, it brings more pain to patients; allogeneic bone transplantation also has immune rejection and carries viruses. And the risk of bacteria, so th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/02A61L27/18A61L27/54A61L27/56
CPCA61L27/02A61L27/18A61L27/54A61L27/56
Inventor 魏坤胡露
Owner SOUTH CHINA UNIV OF TECH
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