Radiosensitizer and preparation method thereof
A technology of sensitizer and water solvent, which is applied in the field of radiotherapy sensitizer and its preparation, can solve the problems of limited improvement of siRNA stability, no risk of viral vectors, failure to meet clinical safety and long-term effect, etc. Achieve the effects of reducing radiosensitivity, stable properties, and easy administration
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Embodiment 1
[0029] A radiotherapy sensitizer, comprising: 80% PLGA-PEI / hypericin nano-micelle, 8% HIF-1α-siRNA, 12% hyaluronic acid, wherein the molecular weight of hyaluronic acid is 11000 . Its preparation process comprises the following steps:
[0030] 1. Weigh 0.2g of Poloxamer 188, dissolve in 10mL of deionized water, and wait to dissolve.
[0031] 2. PEI-acetone stock solution: Weigh 0.2g of polyetherimide (PEI) with a molecular weight of 25,000, Tween 800.05g, a little acetone, dissolve it by ultrasonic and pour it into a 10mL volumetric flask, then add acetone dropwise to volume.
[0032] 3. Weigh 0.2g of PLGA with a molecular weight of 10000, 0.1g of Tween 80, 1mL of PEI-acetone stock solution, 6.5mL of acetone, and 2.5mL of dichloromethane, and ultrasonically dissolve them.
[0033] 4. Dissolve hypericin in ethanol solvent to prepare a 2.5 mg / mL solution.
[0034] 5. Take 40 μL of the hypericin solution prepared in step 4, mix with 10 mL of the poloxamer 188 solution prepared...
Embodiment 2
[0041] A radiotherapy sensitizer, comprising: 50% PLGA-PEI / hypericin nano-micelle, 5% HIF-1α-siRNA, 45% hyaluronic acid, wherein the molecular weight of hyaluronic acid is 6600 . Its preparation process comprises the following steps:
[0042] 1. Weigh 0.2 g of polyvinylpyrrolidone (PVA), and add it gradually into 10 mL of physiological saline under magnetic stirring to obtain a clear PVA solution.
[0043] 2. PEI-acetone stock solution: Weigh 0.2g of polyetherimide (PEI) with a molecular weight of 25,000, Tween 800.05g, a little acetone, dissolve it by ultrasonic and pour it into a 10mL volumetric flask, then add acetone dropwise to volume.
[0044] 3. Weigh 0.2g of PLGA with a molecular weight of 10000, 0.1g of Tween 80, 1mL of PEI-acetone stock solution, 6.5mL of acetone, and 2.5mL of dichloromethane, and ultrasonically dissolve them.
[0045] 4. Dissolve hypericin in ethanol solvent to prepare a 2.5 mg / mL solution.
[0046] 5. Take 40 μL of the hypericin solution prepare...
Embodiment 3
[0053] A radiotherapy sensitizer, comprising: 80% PLGA-PEI / hypericin nano-colloids, 8% HIF-1α-siRNA, 33% hyaluronic acid, wherein the molecular weight of hyaluronic acid is 800 . Its preparation process comprises the following steps:
[0054] 1. Weigh 0.2g of Poloxamer 188, dissolve in 10mL of deionized water, and wait to dissolve.
[0055] 2. PEI-acetone stock solution: Weigh 0.2g of polyetherimide (PEI) with a molecular weight of 25,000, Tween 800.05g, a little acetone, dissolve it by ultrasonic and pour it into a 10mL volumetric flask, then add acetone dropwise to volume.
[0056] 3. Weigh 0.2g of PLGA with a molecular weight of 10000, 0.1g of Tween 80, 1mL of PEI-acetone stock solution, 6.5mL of acetone, and 2.5mL of dichloromethane, and ultrasonically dissolve them.
[0057] 4. Dissolve hypericin in ethanol solvent to prepare a 2.5 mg / mL solution.
[0058] 5. Take 40 μL of the hypericin solution prepared in step 4, mix with 10 mL of the poloxamer 188 solution prepared ...
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