BMP-2/PPLA (bone morphogenetic protein-2/polylactic acid and polyethylene glycol block copolymer) microspheres and preparation method thereof
A technology of BMP-2 and microspheres, which is applied in the direction of microcapsules, pharmaceutical formulations, peptide/protein components, etc., can solve the problems of microspheres with different particle sizes, unstable microspheres, and short half-life, and achieve drug delivery. Diversification, reduced cell adhesion, easy operation and controllable effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0037] Embodiment 1: Preparation of BMP-2-PPLA-MS
[0038](1) Weigh a certain mass of PPLA and dissolve it in dichloromethane, shake and dissolve with a vortex machine, and prepare a solution with a PPLA concentration of 90 mg / ml. Take a certain volume of PPLA (molecular weight is 50000Da) solution and place it in the EP tube, add BMP-2 protein stock solution (concentration is 0.55mg / ml), control the volume ratio of BMP-2 protein stock solution and PPLA solution added to be 1: 5. Use a homogenizer to emulsify for the first time in an ice bath environment, with a shear speed of 15000 rpm and a time of 20 s, to obtain colostrum.
[0039] (2) Weigh a certain mass of PVA and dissolve it in a certain volume of distilled water, swell overnight and stir to dissolve or heat to 80 to 90°C to dissolve, configure it as a solution with a PVA mass fraction of 4%, cool to room temperature after the dissolution is complete, and Add a certain volume of PVA solution to the colostrum obtained ...
Embodiment 2
[0052] Embodiment 2: Preparation of BMP-2-PPLA-MS
[0053] (1) Weigh a certain mass of PPLA and dissolve it in dichloromethane, shake and dissolve with a vortex machine, and prepare a solution with a PPLA concentration of 95 mg / ml. Take a certain volume of PPLA (molecular weight is 50000Da) solution and place it in the EP tube, add BMP-2 protein stock solution (concentration is 0.55mg / ml), control the volume ratio of BMP-2 protein stock solution and PPLA solution added to be 1: 5. Use a homogenizer to emulsify for the first time in an ice bath environment with a shearing speed of 16,000 rpm and a time of 15 s to obtain colostrum.
[0054] (2) Weigh a certain mass of PVA and dissolve it in a certain volume of distilled water, swell overnight and stir to dissolve or heat to 80 to 90°C to dissolve, configure it as a solution with a PVA mass fraction of 4%, cool to room temperature after the dissolution is complete, and Add a certain volume of PVA solution to the colostrum obtain...
Embodiment 3
[0058] Embodiment 3: the preparation of BMP-2-PPLA-MS
[0059] (1) Weigh a certain amount of PPLA and dissolve it in dichloromethane, shake and dissolve with a vortex machine, and prepare a solution with a PPLA concentration of 85 mg / ml. Take a certain volume of PPLA (molecular weight is 50000Da) solution and place it in the EP tube, add BMP-2 protein stock solution (concentration is 0.55mg / ml), control the volume ratio of BMP-2 protein stock solution and PPLA solution added to be 1: 5. Use a homogenizer to emulsify for the first time in an ice bath environment with a shear speed of 14000 rpm and a time of 25 s to obtain colostrum.
[0060] (2) Weigh a certain mass of PVA and dissolve it in a certain volume of distilled water, swell overnight and stir to dissolve or heat to 80 to 90°C to dissolve, configure it as a solution with a PVA mass fraction of 4%, cool to room temperature after the dissolution is complete, and Add a certain volume of PVA solution to the colostrum obta...
PUM
Property | Measurement | Unit |
---|---|---|
concentration | aaaaa | aaaaa |
concentration | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com