Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of regulating microsphere drug-load rate by treating calcium alginate microspheres with sodium chloride solution

A technology of sodium chloride solution and calcium alginate is applied in pharmaceutical formulations, medical preparations of non-active ingredients, and bulk delivery, etc., which can solve the problem that the drug loading of microspheres is not easy to control, and the drug loading and embedding rate are inconsistent. It can achieve the effect of good application prospect, high encapsulation rate and wide source of raw materials.

Inactive Publication Date: 2012-08-15
ZHEJIANG UNIV
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to obtain microspheres with a particle size of less than 50 μm by the electrostatic generation method, while the microspheres obtained by the stirring emulsification method have a wider particle size distribution, and the method of mixing the drug and the microsphere material at the initial stage of preparation has low drug loading and embedding efficiency. , The problem that the drug loading of microspheres is not easy to control, the above shortcomings limit its promotion and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of regulating microsphere drug-load rate by treating calcium alginate microspheres with sodium chloride solution
  • Method of regulating microsphere drug-load rate by treating calcium alginate microspheres with sodium chloride solution
  • Method of regulating microsphere drug-load rate by treating calcium alginate microspheres with sodium chloride solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Prepare 10mL of 2% sodium alginate solution as the water phase, prepare 120mL of isooctane containing 3% emulsifier (Span 85: Tween 85=9:1) as the oil phase, pour the water phase and the oil phase Into a membrane emulsifier containing a cylindrical SPG membrane, the water phase and the oil phase are respectively on the outside and inside of the cylindrical SPG membrane, and pressure is applied with nitrogen gas under stirring to make the water phase squeeze through the SPG membrane and enter the oil phase to obtain an oil-in-oil phase. Water emulsion, add 20mL salt solution containing 5% calcium chloride and 20% sodium chloride to the water-in-oil emulsion, stir for 0.5h, then add 50mL isopropanol and continue stirring for 1h, separate liquid, centrifuge and wash to obtain alginic acid Calcium microspheres, the scanning electron micrographs of which are shown in figure 1 a, b, light microscope photos see figure 1 c;

[0022] 2) Add 2 mg of calcium alginate microsph...

Embodiment 2

[0025] Step is the same as embodiment 1, but adopting massfraction in step 2) is the sodium chloride solution of 0.9%, and its optical microscope photo is shown in figure 2 b, SEM photo after drying see image 3 b, The light microscope and laser confocal photos of the microspheres loaded with doxorubicin are shown in Figure 6 b.

Embodiment 3

[0027] Step is the same as embodiment 1, but adopting massfraction in step 2) is the sodium chloride solution of 1.8%, and its optical microscope photo is shown in figure 2 c, SEM pictures after drying are shown in image 3 c, The light micrograph of the microspheres loaded with doxorubicin is shown in Figure 6 c.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method of regulating microsphere drug-load rate by treating calcium alginate microspheres with sodium chloride solution. The method comprises the following steps: preparing calcium alginate microspheres with uniform grain diameter by adopting a membrane emulsification-gelatinization method; soaking the microspheres with sodium chloride solutions with different concentrations to substitute sodium ions for part of calcium ions in the calcium alginate microspheres, and then cleaning with ultrapure water; and soaking the treated microspheres into medicinal solution with positive charges, and loading drugs by virtue of static effect to obtain the calcium alginate microspheres with different drug-load rates finally. The preparation method is easy and controllable, the material sources are wide, the drug-load rate of microspheres can be conveniently adjusted, and the method has the advantage of excellent application prospect.

Description

technical field [0001] The invention relates to a method for preparing microspheres and regulating their drug loading. Especially the method of treating the microspheres with different concentrations of sodium chloride solutions to regulate the drug loading of the microspheres. Background technique [0002] Polymer microspheres refer to a class of polymer materials with micron-scale diameters and spherical shapes. In recent years, polymer microspheres have been widely studied due to their potential huge application value in the fields of medicine, biochemistry, and electronic information. At present, the main preparation methods of microspheres are spray drying method, extrusion method, interfacial and in-situ polymerization method, phase separation method and multiple phase emulsion method. These methods have large yields, but the particle size distribution of the prepared microspheres is mostly very wide, which limits the application in some fields. Membrane emulsificat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K47/36A61K9/16
Inventor 高长有仝维鋆宋亦超
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com