Dual-hole polysaccharide microspheres, preparation method and purpose thereof

A polysaccharide and microsphere technology, applied in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc., can solve the problems of broken balls, deformation of microspheres, and unnecessary use.

Active Publication Date: 2013-10-09
SENHUI MICROSPHERE TECH SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, some other methods used to prepare macroporous materials, such as repeated melting and freeze-drying, etc., because the process of repeated melting and freeze-drying will have a great impact on the morphology of microspheres, causing deformation and aggregation of microspheres, There are even broken balls, etc., so it is generally not used to prepare macroporous microsphere media

Method used

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  • Dual-hole polysaccharide microspheres, preparation method and purpose thereof
  • Dual-hole polysaccharide microspheres, preparation method and purpose thereof
  • Dual-hole polysaccharide microspheres, preparation method and purpose thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0100] Accurately weigh a certain amount of wet agarose particles (the molecular weight of agarose is 120,000 Daltons, the mass concentration of agarose in the agarose particles is 10wt%, the particle size of the agarose particles is 2 μm, the particle size of the agarose particles The distribution coefficient is 15%, and the particle size distribution diagram is as follows Figure 5 shown), and then disperse the agarose particles into the water phase under magnetic stirring (the water phase is 20wt% ethanol aqueous solution, the volume of the water phase is 1mL, the water phase contains polyvinyl alcohol with a concentration of 0.1wt%, and the water phase The mass concentration of agarose particles is 20wt%), and the magnetic stirring speed is 500rpm. Add the water phase containing agarose particles into the oil phase under mechanical stirring (the oil phase is a mixed solvent of liquid paraffin and petroleum ether, the volume ratio of liquid paraffin to petroleum ether is 11...

Embodiment 2

[0102] Macroporous agarose microspheres were prepared by the same method as in Example 1, but the difference was in the properties of the agarose particles. Accurately weigh a certain amount of wet agarose particles (the molecular weight of agarose is 80,000 Daltons, the mass concentration of agarose in the agarose particles is 4wt%, the particle diameter of the agarose particles is 50nm, the particle diameter of the agarose particles The distribution coefficient is 10%), and then disperse the agarose particles into the water phase under magnetic stirring (the water phase is 50wt% ethanol aqueous solution, the volume of the water phase is 1mL, and the mass concentration of the agarose particles in the water phase is 50wt%) , the magnetic stirring speed is 1000rpm. Add the water phase containing agarose particles into the oil phase under mechanical stirring (the oil phase is a mixed solvent of liquid paraffin and petroleum ether, the volume ratio of liquid paraffin and petroleu...

Embodiment 3

[0105]Double-hole chitosan microspheres were prepared by the same method as in Example 1, except that chitosan particles were used instead. Accurately take a certain amount of wet chitosan particles (the chitosan molecular weight is 780,000 daltons, the mass concentration of chitosan in the chitosan particles is 1.5wt%, and the particle diameter of the chitosan particles is 4 μm, The particle size distribution coefficient of chitosan particles is 12%), and then the chitosan particles are dispersed into the water phase under the effect of homogeneity (the water phase is 40wt% acetone aqueous solution, the volume of the water phase is 1mL, and the shell in the water phase The mass concentration of polysaccharide particles is 70wt%), the homogenization speed is 6000rpm, and the homogenization time is 1min. The aqueous phase containing chitosan particles is added to the oil phase under magnetic stirring (the oil phase is cyclohexane, the volume of the oil phase is 50mL, and the oi...

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Abstract

The invention relates to dual-hole polysaccharide microspheres, a preparation method and a purpose thereof. The microspheres are formed by a plurality of stacked polysaccharide particles with small particle sizes, with an average particle size of the microspheres being 500 nm to 500 [mu]m; and comprises gel pores containing the polysaccharide as well as macroporous dual-hole structure formed by gaps among the polysaccharide particles. The preparation method of the microspheres comprises dispersing the polysaccharide particles in a water phase and then in an oil phase to form water in oil emulsion, heating the emulsion, and stirring to obtain the dual-hole polysaccharide microspheres. Furthermore, according to the preparation method, together with a membrane emulsification technology, the dual-hole polysaccharide microspheres with uniform particle size distribution can be prepared, with an average particle size range being 500 nm to 100 [mu]m and a particle size distribution coefficient being less than 20 %. The dual-hole polysaccharide microspheres provided by the invention are suitable for being used as separation mediums for separating biomacromolecules, biologically active material carriers, etc.

Description

technical field [0001] The present invention relates to a microsphere and its preparation method and application, in particular to a double-porous polysaccharide microsphere and its preparation method and application. Background technique [0002] With the development of life sciences, the separation of related biomacromolecules has become an extremely important topic. At present, the separation and purification technology for large-scale preparation of proteins has become a limiting factor in the development of biotechnology. A lot of research is going on at home and abroad, and this direction has also become one of the focuses of scientific and technological investment. [0003] Although the research on bioseparation media has a history of several decades, the performance of the media (such as mechanical strength, scope of application and service life) and its separation ability have been greatly improved, but the three factors of resolution, capacity and separation speed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61L27/20A61L27/56A61L31/04A61L31/14A61K8/73A61K39/39C08L5/00C08L5/02C08B37/00C08B37/02C08J9/00C08J3/24
Inventor 马光辉吴颉赵希苏志国崔金梅周炜清
Owner SENHUI MICROSPHERE TECH SUZHOU CO LTD
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