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Real-time dynamic light scattering quantitative PCR instrument

A dynamic light scattering and real-time dynamic technology, applied in the direction of instruments, biochemical instruments, measuring devices, etc., can solve the problems of inability to detect target nucleic acid PCR amplification in real time, limit the application of biochemical analysis, etc., achieve reliable measurement results, ensure accuracy, The effect of high degree of automation

Active Publication Date: 2018-10-02
SUN YAT SEN UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the dynamic light scattering instrument cannot detect the PCR amplification of the target nucleic acid in real time, which limits its application in biochemical analysis

Method used

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  • Real-time dynamic light scattering quantitative PCR instrument
  • Real-time dynamic light scattering quantitative PCR instrument
  • Real-time dynamic light scattering quantitative PCR instrument

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Experimental program
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Embodiment 1

[0041] Such as figure 2 The shown one is suitable for the dynamic light scattering detection component in the real-time dynamic light scattering quantitative PCR instrument of the present invention.

[0042] The detection component includes a low-coherence light source 6, where the low-coherence light source 6 uses a super-radiation laser diode. In addition, as the light source, other low-coherence light sources or extremely short-coherent light sources such as white light LEDs can also be used.

[0043] The low-coherence light source 6 is connected to an optical circulator 7, and the optical circulator 7 adopts a three-port optical circulator. The first port of the optical circulator 7 is used as the light source entrance port and is connected to the low-coherence light source 6 through light 73; The second port of the optical circulator 7 is connected to a first transmitter 71 through an optical fiber 73, and the first transmitter 71 outputs the light emitted to the second port t...

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Abstract

The invention discloses a real-time dynamic light scattering quantitative PCR instrument including: a thermal circulation system used for controlling heating status of a PCR sample test tube; a dynamic light scattering detection unit, which includes an irradiation part that irradiates a light from a light source onto the PCR sample test tube, a spectrum intensity acquiring part and a measuring part, wherein the spectrum intensity acquiring part splits a reflecting light from a reference surface and a scattered light which transmits through the reference surface and comes from the sample, thusobtaining the spectrum intensity of the reflecting light and an interference light thereof, then the measuring part, on the basis of the spectrum intensity, performs detection on real-time dynamic light scattering change to the PCR sample; a control system, which includes a control unit, and an upper computer that analyzes and processes a detection data coming from the measuring part. The method can eliminate interference on the detection due to nonspecific PCR products, and can be used for observing the increase status of a PCR amplified product in the reaction tube in real time, is high in automatic degree and is more reliable in detection result.

Description

Technical field [0001] The invention belongs to the technical field of polymerase chain reaction detection, and specifically relates to a real-time dynamic light scattering quantitative PCR instrument. Background technique [0002] Polymerase Chain Reaction (PCR) is an in vitro nucleic acid amplification technology with high specificity, high sensitivity, simplicity and speed. There are two commonly used PCR machines: ordinary PCR machines and real-time fluorescent quantitative PCR machines. Only the PCR amplification of the target nucleic acid can be carried out on the ordinary PCR instrument. The PCR amplification product needs to be in other instruments and devices (such as gel electrophoresis, fluorescence spectrophotometer, ultraviolet-visible spectrophotometer, circular dichroic spectrometer, The detection is carried out on the dynamic light scattering instrument and the electrochemical workstation, and the PCR amplification and signal detection are respectively completed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/34C12Q1/686G01N15/02
CPCG01N15/0205C12Q1/686
Inventor 凌连生邹李
Owner SUN YAT SEN UNIV
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