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Method for screening plant variety with leaf strengthening function

A technology of plant varieties and screening methods, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of short photosynthesis time, inability to measure photosynthesis parameters, short growth period of corn, etc., and achieve the measurement results reliable effect

Inactive Publication Date: 2017-05-10
HUAIAN RES INST OF NANJING AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

However, in breeding, a large number of materials need to be identified and screened, and there has been no suitable method.
The growth period of corn is short, generally about 100 days. The leaves in the late growth period are aging and the leaves in the early growth period are too small to measure. The time suitable for measuring photosynthesis is very short. It is easy to use the LI-6400 instrument to measure photosynthesis parameters due to weather reasons.

Method used

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  • Method for screening plant variety with leaf strengthening function
  • Method for screening plant variety with leaf strengthening function
  • Method for screening plant variety with leaf strengthening function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Preliminary experiments: We conducted related experiments on the effects of different planting densities and different leaf positions on the chlorophyll content of maize leaves and the light response curve of leaves.

[0066] (1) Determination of leaf chlorophyll (Chl) content: take the chlorophyll content of Zhengdan 958 3rd, 6th (clover clover), and 9th leaves (counting from the base) at the jointing stage and filling stage of corn respectively, wherein the 6th leaf Bits of corn on the cob clover. Rinse with water, wipe dry, and set aside. The middle part of the leaf is used as the measurement site. Punch holes in the middle of each leaf with a 9mm diameter puncher, take 6 round leaves, weigh them, put them in a 15ml test tube, soak them in 10ml of 80% acetone for 24 hours at room temperature at 25°C in the dark, during the soaking process After the leaves are completely white, measure the optical density values ​​at 663nm and 645nm respectively with a visible light ...

Embodiment 2

[0072] Example 2 Determination of Leaf Temperature, Nitrogen Content and Chlorophyll Content of Different Maize Varieties in High Temperature Period

[0073] Five corn varieties (Suyu 19, Suyu 30, Jundan 20, Jundan 29 and Zhengdan 958) were planted in random blocks, with 6 rows per plot and a row length of 5 meters. The density is 5000 plants per mu. On July 28, 29, and 30, 2016, when the highest temperature reached 35°C, the temperature, chlorophyll content, and For indicators such as nitrogen content, 10 plants were selected for each material, and the average value was calculated. Select 3 parts of materials with good leaf function (that is, low leaf temperature, high chlorophyll content, and high nitrogen content in high temperature period). Among them, the lower the leaf temperature, the stronger the ability to cool down through transpiration, the higher the enzyme activity of the leaf, the better the function, the lower the leaf temperature, and the greater the differen...

Embodiment 3

[0075] The mensuration of embodiment 3 leaf fluorescence parameter PI (abs)

[0076] On August 5, August 11, and August 17, 2016, at around 20:00 on these three days, under natural conditions, the dark treatment was carried out for more than half an hour. At the flowering stage of corn at the Liuhe test site, the four leaves were selected as the measurement leaves. Su Five varieties of Yu 19, Suyu 30, Zhengdan 958, Jundan 20 and Jundan 29 had 9 replicates for each variety, and PI (abs) (absorbed light energy-based performance index) parameters.

[0077] Such as Pic 4-1 , PI(abs) decreased after the high temperature period. Combined with the weather conditions in Liuhe ( Figure 4-2 ) can be analyzed that before August 11, the temperature did not reach or exceed 35°C, and a high temperature was experienced between the 11th and 17th. PI(abs) began to decrease after reaching the maximum value on the 11th, and PI(abs) decreased under the pressure of high temperature stress. ...

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Abstract

The invention discloses a method for screening a plant variety with a leaf strengthening function. The invention belongs to the fields of breeding of crop high-lighting-effect and high-temperature-resistant variety, and relates to a method for screening a high-light-effect corn variety by a technological system, wherein the technological system is to screen a large amount of corn combination by applying leaf temperature, difference between leaf temperature and air temperature, leaf chlorophyll content, leaf nitrogen content and a fluorescence parameter PI (abs), select about 8 parts of materials, measure the photosynthetic rate by an LI-6400 photosynthesis system at the daily maximum temperature being 35 DEG C or above and select 3 to 4 parts of materials again to measure parameters such as photoresponse and carbon dioxide response. Compared with other breeding methods, the technology has the characteristic of screening a large amount of corn varieties, is suitable for selecting and breeding a high-quality corn variety from a large amount of varieties and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of crop variety breeding. Specifically, the present invention relates to a plant screening method with strong leaf function, especially the screening of corn varieties, specifically related to the application of indicators such as photosynthesis intensity of corn leaves, leaf temperature, chlorophyll content, nitrogen content, and fluorescence parameters PI (abs) A method for comprehensive screening of maize varieties with strong leaf function. Background technique [0002] The trend of global warming is obvious. The IPCC Fourth Assessment Report shows that the global surface temperature has increased by 0.74°C in the past 100 years, and it is predicted that the temperature will increase by 1.1-6.4°C by the end of this world. From 1951 to 2009, China's average temperature rose by 1.38°C, and the warming rate reached 0.23°C per decade. "China's National Program on Climate Change" (2007) pointed out that the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 唐灿明季浩胡启瑞吴颖静许小明
Owner HUAIAN RES INST OF NANJING AGRI UNIV
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