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Rapid identification method for cotton verticillium wilt resistance in seedling stage

A cotton Verticillium wilt and Verticillium wilt technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of complex measurement process, time-consuming, unsuitable for rapid identification, etc. Achieve the effect of speeding up the breeding process, shortening the time, and simplifying the activity of enzymes related to cotton seedling Verticillium wilt

Inactive Publication Date: 2017-12-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the method for judging the disease resistance of cotton seedlings according to the activity of related enzymes after inoculation (a method for detecting the disease resistance of cotton verticillium wilt, application number: CN201510111867.9), the onset of cotton seedlings can be detected and measured without obvious symptoms. The results have certain accuracy, but the enzyme activity determination process is complicated and time-consuming, and it is not suitable for the rapid identification of Verticillium wilt resistance of batch varieties

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  • Rapid identification method for cotton verticillium wilt resistance in seedling stage
  • Rapid identification method for cotton verticillium wilt resistance in seedling stage
  • Rapid identification method for cotton verticillium wilt resistance in seedling stage

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Embodiment Construction

[0047] The present invention will be further described below in conjunction with specific examples.

[0048] 1. Test materials

[0049] Zhongzhimian No. 2, Simian No. 3, Xumian No. 21, Sikang No. 1, Lumianyan No. 28, Lumianyan No. 36, Jimian No. 11 (control variety susceptible to Verticillium wilt), Nongda 601.

[0050] 2. Implementation steps

[0051] (1) Select the above-mentioned plump cotton seeds, soak them in 70% (v / v) alcohol for 5 minutes, and soak them in 5% (w / w) H 2 o 2 Soak for 2 hours, rinse with sterile water for 2 to 3 times, and then soak in sterile water for 10 hours to accelerate germination.

[0052](2) Lay the above-mentioned treated cotton seeds on moist gauze and place them in a controllable artificial climate box. After the cotton seeds are white, select seeds with the same root length and seed size, and sow them in seedling cups ( Length * width * height=8cm * 8cm * 15cm), every cup sows 4 grains, 40 cups of every kind altogether. The seedling rais...

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Abstract

The invention discloses a rapid identification method for cotton verticillium wilt resistance in seedling stage. The rapid identification method comprises the following steps: inoculating greensickness bacteria to cotton seedlings, measuring SPAD value of inoculated cotton leaves and calculating greensickness stress index, and then judging the cotton verticillium wilt resistance according to the greensickness stress index. According to the method, the cotton leaves are not damaged, the method is not disturbed by man-made subjective judgment factors, the measurement result is more reliable compared with a disease index method, the method is simpler and more convenient compared with a method for measuring the activity of cotton seedling greensickness related enzyme, and the measured target is unique. The detection method can rapidly identify the disease resistance of cotton verticillium wilt in each growth stage, the breeding process of varieties with cotton verticillium wilt is quickened, and the detection method can be directly popularized and applied to production.

Description

Technical field: [0001] The invention belongs to the technical field of cotton disease resistance detection, and in particular relates to a method for rapidly identifying cotton verticillium wilt resistance at the seedling stage. Background technique: [0002] Verticillium wilt is one of the seven major soil-borne diseases in the world. It has the characteristics of wide distribution, serious damage and strong infection. It is extremely difficult to cure and is called "cotton cancer". At present, it is recognized internationally that planting disease-resistant varieties is the most economical and effective measure to control Verticillium wilt, and the identification of cotton Verticillium wilt resistance is an important prerequisite for cotton Verticillium wilt-resistant breeding and control. [0003] Verticillium wilt will occur throughout the growth period of cotton. Generally, symptoms begin at the 3rd to 5th leaf stage, and a large number of diseases occur after budding....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12N1/14C12R1/645
CPCC12N1/14C12Q1/18
Inventor 陈兵林张希鹤李岩郁凯霍钰阳周治国王友华孟亚利
Owner NANJING AGRICULTURAL UNIVERSITY
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