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Chromatographic method for determining content of EPA, DHA and total w-3 acids in fish oil

A chromatographic method and w-3 technology, applied in the chromatography of DHA and total w-3 acid content determination, in the field of EPA in fish oil, can solve the problems of solvent volatilization results, abnormality, complicated operation methods, etc., to simplify the operation process and avoid Abnormal measurement results, stable and reliable measurement results

Inactive Publication Date: 2015-05-20
JIANGSU ALAND NOURISHMENT
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Problems solved by technology

The existing operation method is complicated, and the solvent volatilization is prone to occur during the measurement process, resulting in abnormal results

Method used

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  • Chromatographic method for determining content of EPA, DHA and total w-3 acids in fish oil

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with specific embodiments.

[0038] like figure 1 As shown, determination of EPA, DHA and total w-3 acid content in samples of Mega EPA / DHA 300mg softgels:

[0039] (1) Reagents and stock solutions:

[0040] 14% solution of boron trifluoride in methanol

[0041] Methanol, sodium chloride, isooctane, anhydrous sodium sulfate, sodium hydroxide, 2,6-di-tert-butyl-p-cresol (BHT)

[0042] 0.5 N methanol solution of sodium hydroxide: Weigh 2g of sodium hydroxide into a 100ml volumetric flask, add methanol to dissolve and dilute to the mark;

[0043] BHT solution: Weigh 25mg of BHT into a 500ml volumetric flask, add isooctane to dissolve and dilute to the mark;

[0044] Standard sodium chloride solution: Dissolve enough sodium chloride in 100 ml of water (i.e. saturated sodium chloride solution).

[0045] Reference substance: DHA methyl ester, EPA standard substance.

[0046] (2) Preparation of standar...

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Abstract

The invention discloses a chromatographic method for determining the content of EPA, DHA and total w-3 acids in fish oil. The method comprises the following steps: 1, esterifying; 2, preparing a sample solution; 3, preparing a standard substance solution; 4, setting chromatographic conditions; 5, sequentially introducing samples; and 6, comparing the relation between the retention time and the peak area of a sample to be determined with that of a standard substance to determine the content in the sample. In the w-3 fatty acid-containing fish oil sample treatment process, unsaturated fatty acids is saponified, saponified unsaturated fatty acids and a methanol solution of boron trifluoride directly undergo an ester formation reaction, and unsaturated fatty acid esters are directly extracted by a BHT-containing isooctane solution to obtain a solution used as the sample to be determined. The method adopting the appropriate ester formation process and the sample post-treatment process has the advantages of short test operation cycle, effective avoiding of volatilization and other problems in the operation, and accurate and reliable determination result, and is especially suitable for industrial production and other occasions needing large scale determination.

Description

technical field [0001] The invention belongs to the specific application of gas chromatography (GC) in the analysis of food and drug components, and more specifically relates to a chromatographic method for the determination of EPA, DHA and total w-3 acid content in fish oil. Background technique [0002] Fish oil is a natural health food with high utilization value. Its rich w-3 unsaturated fatty acids can help lower cholesterol, prevent blood coagulation, prevent thrombosis, arteriosclerosis and hypertension, reduce blood viscosity, promote blood circulation, eliminate fatigue, relieve gout and rheumatoid arthritis. Among the w-3 unsaturated fatty acids rich in fish oil, especially eicosapentaenoic acid (EPA) (C20: 5 n-3) and docosahexaenoic acid (DHA) (C22: 6 n- 3) The most important. EPA will play a positive role in the treatment of lung disease, kidney disease, type 2 diabetes, large intestine ulcer and Crohn's disease; DHA is the w-3 fatty acid with the highest conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 盛洁包亚君
Owner JIANGSU ALAND NOURISHMENT
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