Corn seed vitality determining method based on Ca<2+> flow rate
A viability measurement and maize seed technology, which is applied in the field of maize seed viability measurement based on Ca2+ flow rate, can solve the problems of great influence on yield, uneven emergence, and no seedlings, and achieves low seed dosage, high accuracy, and easy measurement. Effect
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Embodiment 1
[0038] Example 1: Harvested corn seed vigor and embryo Ca in the same year 2+ flow rate
[0039] Taking maize hybrids Xianyu 335, Zhengdan 958 and their female parents PH6WC and Zheng 58 as research materials, the Ca 2+ flow rate. Depend on figure 2 It can be seen that the Ca among different maize varieties 2+ The flow rates are different, among which the Ca of Xianyu 335 and Zhengdan 958 2+ The flow rate value is higher, and the flow rate values of the two are similar, the Ca of PH6WC 2+ The flow rate value is next, the Ca of Zheng 58 2+ The lowest flow rate value. Difference law of seedling dry weight vigor index and Ca 2+ same flow rate ( image 3 ).
Embodiment 2
[0040] Example 2: Seed vitality and embryo Ca 2+ flow rate
[0041] The seeds of corn hybrids Xianyu 335 and Zhengdan 958 were subjected to artificial aging treatment and "aging + priming" treatment (after aging, they were primed again), and NMT technology was used to detect Ca 2+ flow rate ( Figure 4 ). Seeds of Xianyu 335 after different treatments, the embryo Ca 2+ The flow rate has changed, showing that the seed embryo Ca without any treatment 2+ The flow rate value was the largest, followed by the treatment of "aging + priming", and the seed embryo Ca of aging treatment 2+ The lowest velocity value ( Figure 5 A); The change trend of corresponding seedling dry weight vigor index is the same ( Figure 5 B). However, Zhengdan 958 "aging + priming" treated embryo Ca 2+ There is no significant difference between the flow rate and the aging treatment seeds (the reason is that the effect of the initiation treatment is not good) ( Figure 5 C), the difference in dry we...
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