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Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa

A technology for tissue culture seedlings and willow willow, which is applied in the field of tree cultivation, can solve the problems of reducing the cultivation environment, high cost and energy consumption, and cumbersome operation, and achieve the effect of abundant explants, strong repeatability and stable cultivation conditions

Inactive Publication Date: 2015-02-11
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example is Chinese patent CN101953307A, which discloses a method for producing Fujian Daodi A. clematis through plant tissue culture. The aseptically treated Fujian Daodi A. clematis tissue is carried out in an induction medium for tissue-induction culture to form clusters of buds. , culture period: 10 to 60 days, culture temperature: 10 to 35°C, light culture time: 0 to 24 hours / day, light intensity: 0 to 2000 lux; the induction medium is: MS medium + sucrose 10 to 50g / L+agar 6~8g / L+activated carbon 2.0~3.0g / L+naphthaleneacetic acid 0.1~3.0mg / L+6-benzylaminoadenine 0.1~5.0mg / L, pH value 5.0~7.0, this medium is suitable for The herbaceous plant golden clematis, and when used on a large scale, additional calcium nitrate, banana puree and other substances need to be added, and the pH of the culture environment needs to be lowered. In actual production, there is a large cost and energy consumption, and the operation is cumbersome, requiring a large amount of labor, its applicability to woody plants is unknown

Method used

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  • Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa
  • Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa
  • Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] 1. Preparation of Media

[0127] (1) Adjust the pH of the MS medium to 5.8 with NaOH, add 6.5g of agar powder, sterilize at 121°C for 20 minutes, and cool to 50°C for use; Naphthaleneacetic acid and 6-benzylaminoadenine, use the aperture Sterilize by filtration through a 0.22 μm filter membrane; add 1 g of activated carbon, 0.15 mg of sterilized naphthaleneacetic acid, and 1 mg of 6-benzylaminoadenine to the agar-MS prepared in the above steps, dilute to 1 L, and solidify to complete the preparation.

[0128] 2. Tissue culture process

[0129] Such as figure 1 As shown, the morphological lower end of the unrooted tissue-cultured seedlings of Salix lanceolata was inserted into the medium, and one branch of Salix lanceolata was inoculated in each medium, and placed in a tissue culture room at a temperature of 27°C, light of 3000 lx, and 10 hours of light 100 unrooted tissue cultured seedlings were co-cultivated under this culture condition.

[0130] 3. Tissue culture r...

Embodiment 2

[0133] 1. The preparation of the medium is the same as in Example 1 with the tissue culture process, the difference is only the weight of adding gac, naphthaleneacetic acid and 6-benzylaminoadenine when preparing the medium:

[0134] Activated carbon 2.5g, naphthaleneacetic acid 0.4mg, 6-benzylaminoadenine 0.8mg.

[0135] 2. Tissue Culture Results

[0136] The unrooted tissue-cultured seedlings of Salix japonica just begin to take root after 14 days; the rooting rate is 90%;

Embodiment 3

[0138] 1. The preparation of the medium is the same as in Example 1 with the tissue culture process, the difference is only the weight of adding gac, naphthaleneacetic acid and 6-benzylaminoadenine when preparing the medium:

[0139] Activated carbon 0.75g, naphthaleneacetic acid 0.08mg, 6-benzylaminoadenine 0.5mg.

[0140] 2. Tissue Culture Results

[0141] The unrooted tissue-cultured seedlings of Salix japonica just begin to take root after 14 days; the rooting rate is 90%;

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Abstract

The invention provides a rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa. A culture medium is used for the rooting culture of the un-rooted tissue culture seedlings of salix matsudana var.tortuosa, and every liter of culture medium comprises MS, 0.01 to 1 milligram of NAA (naphthylacetic acid), 0.01 to 1 milligram of 6-BA (6-benzylaminopurine), 30 grams of sucrose, 6.5 grams of agar powder and 0.5 to 3 grams of activated carbon. According to the method, the un-rooted tissue culture seedlings are high in rooting speed and rooting rate; a culture medium formula is not required to be replaced in a tissue culture process, so that tissue culture operation is simplified; the method has higher scientific value, economic value and practical value.

Description

technical field [0001] The invention relates to the field of tree cultivation, in particular to plant tissue culture. Background technique [0002] Dragon claw willow, also known as dragon beard willow, Latin name: Salix matsudana var.tortuosa, family name: Salicaceae Salicaceae, is a deciduous shrub or small tree of the genus Salicaceae that grows in Northeast, North, Northwest, East China and other places , can grow in wetlands and dry lands, and grows fast. It has the characteristics of positive and cold resistance. It is suitable for spring to summer. Its branches are curled and its posture is unique. It is widely used in garden cultivation. But its quantity is limited, and its self-propagation is not easy, so it is subject to certain restrictions in gardening applications, and there is no good propagation method to propagate it on a large scale at present. [0003] The propagation methods of Longclaw willow include seeding method or cutting method. Among them, the sow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张建国饶国栋睢金凯
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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