Method for extracting general flavones of salix matsudana flowers and antioxidation application

An extraction method and a technology of total flavonoids, which are applied to medical preparations containing active ingredients, anti-toxins, and pharmaceutical formulas, etc.

Active Publication Date: 2017-11-17
XINZHOU TEACHERS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is no research report on the flavonoids of willow willow flower at present. The applicant adopts the microwave method to extract the flavonoids from willow willow flower, which improves the extraction efficiency and obtains the DPPH free radical, ABTS free radical and hydroxyl free radical. Active ingredients

Method used

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  • Method for extracting general flavones of salix matsudana flowers and antioxidation application
  • Method for extracting general flavones of salix matsudana flowers and antioxidation application
  • Method for extracting general flavones of salix matsudana flowers and antioxidation application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The drawing of embodiment 1 standard curve

[0023] 1 Materials and methods

[0024] laboratory apparatus

[0025] V-1100D visible spectrophotometer (Shanghai Mapuda Instrument Co., Ltd.); electric heating constant temperature water bath (Shanghai Yuejin Medical Instrument Factory); SHZ-D (Ⅲ) circulating water vacuum pump (Gongyi Yuhua Instrument Co., Ltd.) ; RE-52AA rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); AL204 electronic balance (Mettler-Toledo Instrument Company); LWMC-201 microcomputer microwave chemical reactor (Nanjing Lingjiang Technology Development Co., Ltd.).

[0026] Experimental Materials

[0027] Rutin (rutin), potassium peroxodisulfate, sodium hydroxide, sodium nitrite, aluminum nitrate, 95% ethanol, absolute ethanol, salicylic acid, ferrous sulfate, ascorbic acid, and 30% hydrogen peroxide were purchased from Sinopharm Group Chemical Reagent Co., Ltd.; 2,2′-hydrazine-bis(3-ethylbenzothiazoline-6-sulfonic acid) diamine salt (...

Embodiment 2

[0037] The selection of embodiment 2 ethanol concentration

[0038] Accurately weigh 0.5 g of the processed willow flower powder, and add 10, 30, 50, 60, 70, and 90% ethanol solutions at a solid-liquid ratio of 1:20 (g / mL). The extraction was carried out for 10 min under the condition of microwave power of 325W. Suction filtration, washing, and constant volume in a 50mL volumetric flask. Parallel 3 times under the same conditions, measure the absorbance according to the method under item 1.3.3 in Example 1, and calculate the flavonoid content.

[0039] For the results of ethanol concentration selection see figure 2 ,Depend on figure 2 It can be seen that: when the ethanol concentration is 10% to 60%, the flavonoid content increases with the increase of the concentration; if it is greater than 60%, the flavonoid content decreases with the increase of the concentration instead, so the preferred ethanol concentration is 60%.

Embodiment 3

[0040] The selection of embodiment 3 microwave power

[0041] Accurately weigh 0.5 g of the processed willow flower powder, and add 60% ethanol solution with a solid-liquid ratio of 1:20 (g / mL). Extract for 10 minutes under the microwave power of 65, 195, 325, 455, 585W. Suction filtration, washing, and constant volume in a 50mL volumetric flask. Parallel 3 times, measure the absorbance according to the method under item 1.3.3 in Example 1, and calculate the flavonoid content.

[0042] The size of the microwave power represents the size of the microwave radiation intensity. The greater the intensity of microwave radiation, the easier it is to decompose the cell wall fibers, which helps the dissolution of the extract and increases the extraction effect; however, if the microwave power is too high, it is easy to cause local overheating and the denaturation of the extract, thereby reducing the extraction effect.

[0043] Depend on image 3 It can be seen that within the range...

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Abstract

The invention discloses a method for extracting general flavones of salix matsudana flowers through assistant microwave extraction, and the antioxidation activity of the general flavones is researched. On the basis of single-factor exploration experiments, process optimization of the level of single-factor experiment selection is achieved through orthogonal experiments, and results show that the general flavones of salix matsudana flowers have certain scavengingfunctions on hydroxyl free radicals, DPPH (Diphenyl Picryl Hydrazinyl) free radicals and ABTS (2,2'-Azino-Bis(3-Ethylbenzthiazoline-6-Sulfonic Acid)) free radicals.

Description

Background technique [0001] Salix matsudana Koidz (Salix matsudana Koidz) is the flower of Salix matsudana Koidz, a deciduous tree belonging to Salicaceae (Salicaceae). Willow flower is also known as "Yanghua", "Willowgrass" and "Liu Rui". Willow flower can be used as both medicine and food, and it has the effect of calming the nerves and hypnosis when used as a pillow. If the willow flower is ground, it can be used for medicine. "Medicine Properties" records: mashing willow flowers can be used to stop bleeding, paste sores, and treat toothache. Flavonoids are a class of main active ingredients in willow flowers, with various types, including luteolin, quercetin, dihydrokaempferol, catechin, isorhamnetin, etc. Modern pharmacology shows that willow flavonoids have anti-inflammatory, anti-cancer, anti-oxidation and other effects. The literature shows that the total flavonoids of willow flower has a certain protective effect on myocardial ischemia-reperfusion injury. [0002...

Claims

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Application Information

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IPC IPC(8): A61K36/76A61P39/06
CPCA61K36/76A61K2236/15A61K2236/333A61K2236/39
Inventor 杨洁索习东范建凤赵三虎赵二劳
Owner XINZHOU TEACHERS UNIV
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