The excavation method of the dry willow -resistant salt hub gene

A salt-tolerance and pivot technology, which is applied in the field of excavation of salt-tolerance pivot genes of Salix chinensis, can solve the problem of inability to accurately find salt-tolerance genes, and achieve the effect of good breeding and improving breeding efficiency.

Active Publication Date: 2020-12-25
NANTONG UNIVERSITY
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Problems solved by technology

[0005] In order to overcome the shortcomings of the existing technology, a method for discovering salt-tolerant pivotal genes of d...

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  • The excavation method of the dry willow -resistant salt hub gene
  • The excavation method of the dry willow -resistant salt hub gene
  • The excavation method of the dry willow -resistant salt hub gene

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[0045] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0046] The invention provides a method for excavating the salt-tolerant pivotal gene of willow, comprising:

[0047] S1: The offspring F is obtained by crossing the salt-tolerant and salt-sensitive Salix willow 1 group.

[0048] In this example, salt-sensitive dry willow is a dry willow that can only grow normally in an environment with a salt content below 2‰. Salt-tolerant dry willow is a dry willow that can grow in an environment...

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Abstract

The invention provides a method for exploring a salt-tolerant hub gene of salix matsudana. The method comprises the following steps: hybridizing salt-tolerant salix matsudana with salt-sensitive salixmatsudana to obtain a filial generation F1 group, and dividing the filial generation F1 group into a salt-tolerant filial generation and a salt-sensitive filial generation; transcriptome sequencing is carried out on the root systems of the salt-tolerant filial generation and the salt-sensitive filial generation respectively to obtain differential expression gene sequences, and a high-density genetic map is constructed; salt tolerance QTLs positioning is carried out, and a corresponding QTL section is obtained through calculation; the QTL section and the differential expression gene sequence are compared with willow whole genome, a plurality of salt-tolerant candidate genes are obtained through analysis and the expression quantity of the salt-tolerant candidate genes is measured; a heterokinetic growth model is established, a system of ordinary differential equations is constructed, a regulation and control network is formed by the salt-tolerant candidate genes, and a salt-tolerant hubgene in the regulation and control network is determined. The problem that salt-tolerant genes cannot be accurately found by traditional salt-tolerant willow breeding is solved.

Description

technical field [0001] The invention relates to the technical field of forest tree breeding, in particular to a method for excavating key salt-tolerant genes of willow. Background technique [0002] Salt-tolerant genes regulate the salt-tolerant phenotype of forest trees by regulating the action of proteins. [0003] The traditional methods of discovering salt-tolerant genes are through comparison of homologous gene sequences or comparison of transcriptome sequencing. These methods cannot accurately find the key genes of forest salt tolerance. At the same time, considering the characteristics of forest trees such as their long growth cycle, complex genetic background, high heterozygosity, weak research foundation, and high noise and sparsity of omics data, traditional methods have relatively large limitations and cannot meet the needs of discovering salt-tolerant genes in forest trees. [0004] Salix matsudana (Salix matsudana) is an important species of Salix genus Salix. ...

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Application Information

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IPC IPC(8): C12Q1/6895G16B20/00
CPCC12Q1/6895C12Q2600/13C12Q2600/158G16B20/00
Inventor 张健江钰娜高俊峰姜立波刘国元余春梅陈艳红连博琳柯勇超
Owner NANTONG UNIVERSITY
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