The excavation method of the dry willow -resistant salt hub gene

Abstract

The invention provides a method for exploring a salt-tolerant hub gene of salix matsudana. The method comprises the following steps: hybridizing salt-tolerant salix matsudana with salt-sensitive salixmatsudana to obtain a filial generation F1 group, and dividing the filial generation F1 group into a salt-tolerant filial generation and a salt-sensitive filial generation; transcriptome sequencing is carried out on the root systems of the salt-tolerant filial generation and the salt-sensitive filial generation respectively to obtain differential expression gene sequences, and a high-density genetic map is constructed; salt tolerance QTLs positioning is carried out, and a corresponding QTL section is obtained through calculation; the QTL section and the differential expression gene sequence are compared with willow whole genome, a plurality of salt-tolerant candidate genes are obtained through analysis and the expression quantity of the salt-tolerant candidate genes is measured; a heterokinetic growth model is established, a system of ordinary differential equations is constructed, a regulation and control network is formed by the salt-tolerant candidate genes, and a salt-tolerant hubgene in the regulation and control network is determined. The problem that salt-tolerant genes cannot be accurately found by traditional salt-tolerant willow breeding is solved.

Description

technical field [0001] The invention relates to the technical field of forest tree breeding, in particular to a method for excavating key salt-tolerant genes of willow. Background technique [0002] Salt-tolerant genes regulate the salt-tolerant phenotype of forest trees by regulating the action of proteins. [0003] The traditional methods of discovering salt-tolerant genes are through comparison of homologous gene sequences or comparison of transcriptome sequencing. These methods cannot accurately find the key genes of forest salt tolerance. At the same time, considering the characteristics of forest trees such as their long growth cycle, complex genetic background, high heterozygosity, weak research foundation, and high noise and sparsity of omics data, traditional methods have relatively large limitations and cannot meet the needs of discovering salt-tolerant genes in forest trees. [0004] Salix matsudana (Salix matsudana) is an important species of Salix genus Salix. ...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the shortcomings of the existing technology, a method for discovering salt-tolerant pivotal genes of dry willow is now provided to solve the problem that traditional salt-tolerant willow breeding cannot accurately find salt-tolerant genes

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