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Method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana

A heavy metal, high resistance technology, applied in the field of bioengineering, can solve the problems of long repair time, limited phytoremediation effect, small biomass, etc., to achieve wide practicability, improve transport capacity and resistance, high transport capacity and resistance effect

Inactive Publication Date: 2019-04-12
TIANJIN NORMAL UNIVERSITY
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, most of the hyperaccumulator plants found are herbaceous, with small aboveground biomass, less accumulation and transfer of heavy metals, long restoration time and low efficiency, which directly limit the effect of phytoremediation, so heavy metal hyperaccumulation plants are generally only As a material for the study of heavy metal absorption and tolerance mechanism

Method used

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  • Method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana
  • Method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana
  • Method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana

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Embodiment Construction

[0036] In order to enable those skilled in the art to better understand the solution of the present invention, the technical solution of the present invention will be further described below in conjunction with specific examples.

[0037] Various raw materials and various equipments used in the present invention are conventional commercially available products, and can be directly obtained through market purchase. The sources of the biological materials used in the examples are as follows: Wild-type tobacco is stored in the laboratory of the School of Life Sciences, Tianjin Normal University. Escherichia coli DH5α and Agrobacterium C58 were purchased from Shanghai Weidi Biotechnology Co., Ltd. The quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

[0038] The gene of the present invention is derived from the ZIP gene of Salix matsudana Koidz., the GENBANK number is KU921227, and its full-le...

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Abstract

The invention discloses a method for improving heavy metal Cd transporting ability and resistance of plants by transforming SmZIP protein of salix matsudana. The method employs a genetic engineering technology to transfer a gene to a wild type tobacco, so that the gene can be specifically expressed in plant cells, and significantly improve the heavy metal Cd transporting ability and resistance ofplants. The method of the present invention has wide applicability, and a novel plant variety having high Cd transport ability and resistance can be obtained.

Description

[0001] The invention is supported by: Tianjin Natural Science Foundation (17JCYBJC22500); Tianjin University Science and Technology Development Project (20110603); Tianjin Normal University Doctoral Fund and Tianjin Animal and Plant Resistance Key Experiment Open Fund. technical field [0002] The invention belongs to the technical field of bioengineering, and in particular relates to a method for improving plant heavy metal Cd transport capacity and resistance by transforming the SmZIP protein of Salix sativa. Background technique [0003] Heavy metal Cd pollution is a serious threat to the ecological environment and human health. The methods to control heavy metal pollution are divided into physical restoration, chemical restoration and biological restoration. It has become a hot field of research and development at home and abroad. So far, more than 450 kinds of heavy metal hyperaccumulators have been discovered. However, most of the hyperaccumulator plants found are her...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/82A01H6/20A01H6/60A01H6/54A01H6/46
CPCC07K14/415C12N15/8271
Inventor 邹金华薛文秀尚晓硕李崇豪王嘉玥刘祥君
Owner TIANJIN NORMAL UNIVERSITY
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