The invention relates to the field of tobacco pest control, in particular to an automatic aphidius gifuensis pupa quantitative packaging machine. The automatic aphidius gifuensis pupa quantitative packaging machine consists of a rack, an electrical controller, an air pump, a negative pressure air valve, a positive pressure air valve, an air pipe, a support, a bearing pedestal, a blowing-suction roll, a blowpipe, a micro motor, a storage box, a feeding box, a numerical control sliding table and an aphidius gifuensis pupa hopper. Automatic quantitative packaging before aphidius gifuensis pupa seedlings are released can be realized automatically, and the production efficiency is improved. In addition, according to the characteristic that aphidius gifuensis pupas are delicate and vulnerale, positive pressure air and negative pressure air are adopted for quantitative packaging and storage of the aphidius gifuensis pupas, the aphidius gifuensis pupas can not be harmed by machines in a packaging and storage process, and the aphidius gifuensis pupa seedlings serve for aphid control work better.