Magnesium ion-enriched bone scaffold material and preparation method thereof

A magnesium ion and bone scaffold technology is applied in the field of magnesium ion-enriched bone scaffolds and its preparation, and can solve the problems of high requirements for auxiliary degradable chemical materials, increased production costs, and the like

Inactive Publication Date: 2017-12-12
THE UNIV OF HONG KONG SHENZHEN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] As can be seen from the above, in the prior art, the implantable bone scaffold made of magnesium element and other auxiliary degradable chemical materials is to prevent the magnesium in the magnesium substrate from degrading too fast, and other materials are used. Chemical materials are released slowly, therefore, the requirements for auxiliary degradable chemical materials are high, and the release rate of magnesium element also needs to be precisely controlled, which increases the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The raw material used in this embodiment is the cancellous bone part of bovine femur, but the realization of the present invention is not limited to bovine bone, any cancellous bone can be used. During the experiment, the bovine bone was sliced ​​sequentially, the oil was removed, the virus was inactivated, and then the bovine bone was decellularized with a decellularized solution composed of trypsin, activator and degreasing agent. After decellularization, bovine bones were dried under vacuum and weighed. Soak the weighed decellularized bovine bone in the magnesium sulfate solution, take it out after a period of time, and obtain the decellularized bovine bone enriched with magnesium sulfate. Then the decellularized bovine bone enriched with magnesium sulfate is rinsed in clean water, taken out after 0.5 hours, dried under vacuum until the mass no longer changes, then weighed, calculated, and sterilized by irradiation, The dose of irradiation is 25KGy~50KGy.

[0032] ...

Embodiment 2

[0041] It is found in Example 1 that when the concentration of magnesium ions is close to saturation, the content of magnesium ions enriched in bovine bone decreases instead. In this embodiment, the method of temperature change is used to enrich magnesium ions in bovine bone. Table 3 is the experimental data of this embodiment. Among them, group A is the change group, group B is the control group 1, and group C is the control group 2.

[0042] In group A, the initial concentration of the solution is 2.5mol / L, the initial temperature of the solution is 90°C, and the temperature of the solution gradually decreases within 4 hours, from 90°C to 25°C, the amount of magnesium ions enriched in the bovine bone; B Group C is the initial concentration of the solution is 2.5mol / L, and the solution temperature is maintained at 25°C, the amount of magnesium ions enriched in the bovine bone within 4 hours; Group C is the initial concentration of the solution is 1.5mol / L, and the solution te...

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PUM

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Abstract

The invention relates to the technical field of bioengineering, in particular to a magnesium ion-enriched bone scaffold material and a preparation method thereof. Magnesium is enriched in decellularized biological bones, so that both natural bones and magnesium ions can play a role in bone repair. Meanwhile, natural bones have large surface areas due to highly void structures, so that magnesium ions can be easily attached to the surface layers of natural bones, osteoblasts can be better induced to adhere to a bone scaffold and grow inside the bone scaffold, and additional supplementation of magnesium in other ways is not needed.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a bone scaffold enriched with magnesium ions and a preparation method thereof. Background technique [0002] Bone defects caused by severe trauma, bone tumors, osteomyelitis and other reasons are very common. The usual treatment for bone defects is bone graft repair. As matrix materials for bone defect repair and tissue engineering, they can be divided into the following categories: Natural bone and its derivatives; artificial synthetic materials; composites of various materials, etc. [0003] In the prior art, the decellularized bone scaffold is to process the bone tissue, remove the cell antigen components in it, retain the extracellular matrix and its three-dimensional scaffold structure, provide a place for growth and metabolism of the host cells, and induce the host cells to grow into , to promote the growth of autologous osteoblasts in its scaffold, so as to complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/02A61L27/56
CPCA61L27/025A61L27/3608A61L27/365A61L27/3687A61L27/56A61L2430/02A61L2430/40
Inventor 王文浩杨伟国
Owner THE UNIV OF HONG KONG SHENZHEN HOSPITAL
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