The invention discloses a gold leaf acer negundo twig cutting seedling method which comprises the steps of cutting bed preparation, cutting stem selection, cutting shoot making, cutting shoot hormone rooting induction, cutting, post-cutting management and the like. Perlite is adopted as a substrate of a cutting bed, and a magnetic field network, an electric field network and an automatic atomizing system are arranged; cutting stems are selected within May, June and July; cutting stems with the diameter ranging from 4 mm to 6 mm are selected as cutting shoots and soaked in a growth regulating agent for 1 min to 2 h; a straight cutting method is adopted for cutting the cutting shoots into the seedbed, the automatic atomizing system carries out automatic atomization after cutting is finished when the air humidity is smaller than 90%; in the period from cutting to callus forming, the shading rate is kept 75%, the relative humidity is kept from 90% to 91%, and the temperature is kept from 30 DEG C to 40 DEG C; in the middle and later periods of cutting, the air humidity is controlled to range from 70% to 80%, the temperature is controlled to range from 30 DEG C to 40 DEG C, and the cutting shoots are processed by 100000 V voltage for 2 h every day. According to the gold leaf acer negundo twig cutting seedling method, the cutting environment most suitable for growth of gold leaf acer negundo twigs is obtained through screening by adjusting and controlling cutting shoot obtaining time, the cutting environment, the cutting shoot selection, the cutting substrate selection, the selection of hormone promoting rooting and other aspects, and the method is stable and efficient for gold leaf acer negundo twig cutting propagation.