Method for collecting and identifying trichoderma fungal volatiles
An identification method and technology of Trichoderma, applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of difficult collection, few types of volatile components, and no volatile components detected grading problem, to achieve the effect of good air tightness, increase enrichment, and prolong service life
Inactive Publication Date: 2019-03-19
SOUTH CHINA AGRI UNIV
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[0006] For this reason, the embodiment of the present invention provides an easy-to-operate, fast, accurate and effective method for collecting and identifying the volatiles of Trichoderma fungi, so as to solve the difficulties in collecting and identifying the volatiles of Trichoderma fungi in the prior art due to the small amount of volatiles. The problem that volatile components cannot be detected or the types of volatile components detected are few, the present invention provides a certain theoretical basis for studying the relationship between Trichoderma fungi and soil-dwelling organisms, and is conducive to dynamic monitoring and control of Trichoderma at the same time Subterranean pests with fungal tropism
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[0052] The collection and identification method of the Trichoderma fungus volatile matter of the present embodiment may further comprise the steps:
[0053] 1) Preparation of culture medium: Mix 36g of potatoes, 3.6g of glucose, 2.7g of agar and 1000ml of pure water, and sterilize in an autoclave at 121°C for 20 minutes. into a Petri dish with a diameter of 90 mm.
[0054] Cultivation of Trichoderma fungi: After the culture medium is cooled, inoculate the spores of Trichoderma fungus on the culture medium in a "Z" shape with a sterile spreading stick, seal the petri dish with sealing tape, and then put the petri dish into the 28 Cultivate in a constant temperature incubator at ℃ for 3-7 days under full light until the spores and hyphae of the fungus cover the petri dish. At the same time, the culture medium not inoculated with Trichoderma fungus was used as a control, which was sealed and placed in an incubator under the same conditions for cultivation.
[0055] 2) Collectio...
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The embodiment of the invention discloses a method for collecting and identifying trichoderma fungal volatiles and relates to the technical field of detection of trichoderma fungal volatiles. The method disclosed by the invention comprises the following steps: enriching the trichoderma fungal volatiles by adopting improved solid phase microextraction (SPME), and detecting components of the trichoderma fungal volatiles by adopting gas chromatography-mass spectrometry (GC-MS). The problem in the prior art that the components of the volatiles cannot be detected or the detected components of the volatiles are less in types because the trichoderma fungal volatiles are small in amount and difficult to collect can be solved. Real chemical information of the trichoderma fungal volatiles can be rapidly and accurately determined, comprehensive and reliable data is provided for conducting a subsequent entomological experiment, dynamic monitoring and control of soil insects can be facilitated, anda theoretical basis is provided for researching interaction between trichoderma fungi and other creatures.
Description
technical field [0001] The embodiment of the present invention relates to the technical field of detection of volatiles of Trichoderma fungi, in particular to a method for collecting and identifying volatiles of Trichoderma fungi. Background technique [0002] Trichoderma (Trichoderma) fungi belong to the half-knowledge fungi in classification, Polypodiaceae, Polypodiaceae, Polychopodiaceae Monocellae, Cephalosporins, mainly exist in forests, slope ditches, farmland, In humid ecological environments such as grasslands, plant residues such as soil, litter and decayed wood, and fruiting bodies of other fungi are its growth substrates. Trichoderma fungi have antagonistic and hyperparasitic effects on plant pathogenic fungi, and are widely used in the prevention and control of various plant fungal diseases, and play an important role in agricultural production. [0003] In the research of the interaction between insects and microorganisms, the inventor team found that undergrou...
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IPC IPC(8): C12P7/26C12P7/04C12P7/24C12P17/06C12N1/14A01N63/04A01P19/00C12R1/885
CPCA01N63/30C12N1/14C12P7/04C12P7/24C12P7/26C12P17/06
Inventor 文超王偲熊鸿鹏温秀军
Owner SOUTH CHINA AGRI UNIV
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