9results about How to "Catalytic strong" patented technology

The invention provides a novel nano material as well as an immobilized enzyme and application thereof, and relates to the fields of biotechnology and application, material synthesis and application, water environment treatment and environmental protection. The particle size of the nano material is 2-30 microns, and the aperture of the surface is 2-30 nm; the solid nanomaterial is prepared by the following method: adding an alkali solution into a metal salt solution, fully mixing, then adding a ligand aqueous solution, fully reacting, and then separating to obtain the solid nanomaterial. When the material is used for immobilizing enzyme, the particle size of the obtained immobilized enzyme is 2-20 microns, and the aperture of the surface is 1-15 nm; the preparation method comprises the following steps: mixing an enzyme with a ligand aqueous solution, then adding the mixture into a metal salt mixed solution, and separating a solid product. The nano material has excellent biocompatibility and mild synthesis conditions, can protect the reaction activity of the enzyme under harsh conditions such as high temperature, acid and alkali, and improves the recycling capability and storage capability of the enzyme.

The invention discloses multi-walled carbon nanotube functionalized sludge biochar as well as a preparation method and application thereof. The method comprises the following steps: firstly, mixing sludge biochar prepared by anaerobic pyrolysis with a multi-walled carbon nanotube, and carrying out ball milling treatment, so as to prepare the sludge biochar functionalized by the multi-walled carbon nanotube; the multi-walled carbon nanotube functionalized sludge biochar prepared by the invention can efficiently activate peroxymonosulfate to degrade trace antibiotics in a water body, and the removal rate of sulfamethoxazole can reach 96% when the reaction is carried out for 60 minutes. According to the method, harmless treatment and resource utilization of the sludge can be realized, and meanwhile, efficient removal of antibiotics in the water body is also realized.

The invention discloses a nano high-entropy phosphide / carbon composite material and a preparation method thereof, and the preparation method comprises the following operation steps: (1) soaking phosphorus-containing resin in an acid solution to adjust the pH value of the phosphorus-containing resin, drying and crushing; (2) adding water, fully stirring to obtain a mixture, uniformly mixing Fe salt, Co salt, Ni salt, Cu salt and Mo salt solutions, adding into the mixture, and recording as M + resin; and (3) adding an alkaline substance, grinding, carrying out heat treatment, and obtaining the nano high-entropy phosphide / carbon composite material after the reaction is finished. According to the method, the phosphorus-containing resin is adopted as the raw material, the adopted resin is environmentally friendly and low in price, and the raw material and preparation cost is low; the method disclosed by the invention is simple to operate, and the particle size of the prepared nano high-entropy phosphide / carbon composite material reaches a nano scale and is controllable.

To address the shortcomings of existing technologies, this invention provides a method for preparing a coprecipitated copper-manganese catalyst and a method for preparing ultrapure nitrogen. The method for preparing the coprecipitated copper-manganese catalyst includes the following steps: mixing copper and manganese metal salt solutions with an oxidant, adjusting the pH to 7.5-11, wherein the molar ratio of copper to manganese is (0.02-3):1; ultrasonically aging the coprecipitate for 20-40 minutes, followed by aging for 10-14 hours; obtaining a filter cake after filtration and washing; drying the filter cake overnight at 95℃-125℃, and then calcining it at 350℃-450℃ in a gaseous atmosphere for 3-5 hours to obtain the coprecipitated copper-manganese catalyst. The catalyst prepared by the method of this invention exhibits good catalytic activity, capable of simultaneously catalytically removing carbon monoxide and hydrogen at room temperature, maintaining a 100% H2 conversion rate for 4-8 hours, and maintaining a high CO conversion rate for more than 50 hours, demonstrating a very long effective duration that meets the long-term operation requirements of industrial production.

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to an activity-improved UDP glycosyl transferase mutant and application thereof. The glycosyl transferase mutant is a four-combination mutant N2HN1I, and the amino acid sequence of the glycosyl transferase mutant is as shown in SEQ ID NO. 4. The mutant provided by the invention has higher catalytic activity, the conversion rate of a substrate crocetin is high, and the mutant has important industrial application value.

The application discloses a vanadium salt hetero-support and a preparation method and application thereof, and belongs to the field of material preparation and catalysis technology. The vanadium oxide-vanadium carbide hetero-support is prepared by a precursor method, vanadium salt is uniformly mixed with an organic carbon source, stirring and dissolving are conducted, drying and filtering are conducted, and a vanadium oxide-vanadium carbide hetero-support precursor is obtained, and the precursor is calcined in an argon or nitrogen atmosphere to obtain the vanadium salt hetero-support. The raw material used is low in price, the process is simple, no flammable and explosive gas is generated in the calcination process, the safety factor is high, the target product is generated by one-step calcination, impurities are not easily introduced, and the repeatability is high. The method is based on the theory of strong and weak metal carrier interaction, and a two-component vanadium salt hetero-support is used as a catalyst base, the catalyst sintering problem caused by Ostwald ripening, particle aggregation and migration is overcome, and thus a catalyst with good stability and good catalytic performance is obtained.

The application discloses an alcohol dehydrogenase mutant and application thereof in synthesis of furfuryl alcohol, and the amino acid sequence of the alcohol dehydrogenase mutant is shown in SEQ ID NO. 3. The mutant YahK-Y114W improves the preference for coenzyme NADH, so that the catalytic efficiency of the mutant for furfural can reach the level of a wild type using coenzyme NADPH. Wet mycelium expressing the mutant YahK-Y114W is used as a biocatalyst, 500 mM furfural is used as a substrate, 500 mM glucose is used as a co-substrate, glucose dehydrogenase is used as an auxiliary enzyme, and NADH is used as a coenzyme to construct a reaction system, and the reaction is carried out at 30 DEG C and 600 rpm for 6 h; the yield of furfuryl alcohol can reach 76.04%, compared with the wild type enzyme, the yield is increased by 12.59%, the preference of the alcohol dehydrogenase for the coenzyme is successfully changed, and the catalytic activity is greatly improved.

The invention discloses a secondary battery and a preparation method thereof, the secondary battery is composed of a positive plate, a negative plate, a diaphragm and an electrolyte, the positive electrode material of the positive plate is composed of a positive active main material and a modified zirconium oxide on the surface of the positive active main material; and a negative electrode material of the negative electrode plate consists of a negative electrode active main material and transition metal nanoparticles on the surface of the negative electrode active main material. The preparation method is used for preparing the secondary battery. According to the invention, the first charge-discharge efficiency and the rate charge-discharge performance of the layered oxide type battery can be effectively improved.

The present application relates to a NADH-dependent L-phosphinothricin dehydrogenase mutant, a construction method and its application in the enzymatic preparation of L-phosphinothricin. The amino acid sequence of the NADH-dependent L-phosphinothricin dehydrogenase mutant is a replacement of the amino acid residues corresponding to the 143rd and / or 353rd positions of the wild-type sequence. The genetically engineered bacteria E. coli BL21 (DE3) / pET30a-PPTDH (A143S / M353V) constructed by the mutant has the property of high yield of L-phosphinothricin, which is used to catalyze the reductive amination reaction of L-phosphinothricin intermediate 2-carbonyl-4-[hydroxy (methyl) phosphono] butyric acid, and can reach a maximum conversion rate of 99.5% within 6 hours at a substrate concentration of 500mM, and the conversion efficiency is much higher than that of the wild-type PPTDH. At the same time, the mutant can efficiently utilize the cheap cofactor NADH for catalytic reaction, and can be applied to the efficient and green preparation of L-phosphinothricin by microbial enzyme method.