4results about How to "Catalytic strong" patented technology

To address the shortcomings of existing technologies, this invention provides a method for preparing a coprecipitated copper-manganese catalyst and a method for preparing ultrapure nitrogen. The method for preparing the coprecipitated copper-manganese catalyst includes the following steps: mixing copper and manganese metal salt solutions with an oxidant, adjusting the pH to 7.5-11, wherein the molar ratio of copper to manganese is (0.02-3):1; ultrasonically aging the coprecipitate for 20-40 minutes, followed by aging for 10-14 hours; obtaining a filter cake after filtration and washing; drying the filter cake overnight at 95℃-125℃, and then calcining it at 350℃-450℃ in a gaseous atmosphere for 3-5 hours to obtain the coprecipitated copper-manganese catalyst. The catalyst prepared by the method of this invention exhibits good catalytic activity, capable of simultaneously catalytically removing carbon monoxide and hydrogen at room temperature, maintaining a 100% H2 conversion rate for 4-8 hours, and maintaining a high CO conversion rate for more than 50 hours, demonstrating a very long effective duration that meets the long-term operation requirements of industrial production.

The invention belongs to the field of gene engineering and enzyme engineering, and particularly relates to an activity-improved UDP glycosyl transferase mutant and application thereof. The glycosyl transferase mutant is a four-combination mutant N2HN1I, and the amino acid sequence of the glycosyl transferase mutant is as shown in SEQ ID NO. 4. The mutant provided by the invention has higher catalytic activity, the conversion rate of a substrate crocetin is high, and the mutant has important industrial application value.

The application discloses a vanadium salt hetero-support and a preparation method and application thereof, and belongs to the field of material preparation and catalysis technology. The vanadium oxide-vanadium carbide hetero-support is prepared by a precursor method, vanadium salt is uniformly mixed with an organic carbon source, stirring and dissolving are conducted, drying and filtering are conducted, and a vanadium oxide-vanadium carbide hetero-support precursor is obtained, and the precursor is calcined in an argon or nitrogen atmosphere to obtain the vanadium salt hetero-support. The raw material used is low in price, the process is simple, no flammable and explosive gas is generated in the calcination process, the safety factor is high, the target product is generated by one-step calcination, impurities are not easily introduced, and the repeatability is high. The method is based on the theory of strong and weak metal carrier interaction, and a two-component vanadium salt hetero-support is used as a catalyst base, the catalyst sintering problem caused by Ostwald ripening, particle aggregation and migration is overcome, and thus a catalyst with good stability and good catalytic performance is obtained.

The application discloses an alcohol dehydrogenase mutant and application thereof in synthesis of furfuryl alcohol, and the amino acid sequence of the alcohol dehydrogenase mutant is shown in SEQ ID NO. 3. The mutant YahK-Y114W improves the preference for coenzyme NADH, so that the catalytic efficiency of the mutant for furfural can reach the level of a wild type using coenzyme NADPH. Wet mycelium expressing the mutant YahK-Y114W is used as a biocatalyst, 500 mM furfural is used as a substrate, 500 mM glucose is used as a co-substrate, glucose dehydrogenase is used as an auxiliary enzyme, and NADH is used as a coenzyme to construct a reaction system, and the reaction is carried out at 30 DEG C and 600 rpm for 6 h; the yield of furfuryl alcohol can reach 76.04%, compared with the wild type enzyme, the yield is increased by 12.59%, the preference of the alcohol dehydrogenase for the coenzyme is successfully changed, and the catalytic activity is greatly improved.