The invention provides a preparation method of a fusion
enzyme and the fusion
enzyme, and the preparation method of the fusion
enzyme comprises the following steps: firstly constructing a fusion enzyme
plasmid, then extracting the fusion enzyme
plasmid, and transferring the fusion enzyme
plasmid into
pichia pastoris for
fermentation expression; and preparing a crude enzyme solution by using
pichia pastoris, and separating and purifying the fusion enzyme. A 6 * His-SUMO tag is fused at the C ends of mutants JaWa, SoLo and WamPa of non-specific
peroxidase (UPO), the fusion enzyme is subjected to induced expression in
pichia pastoris and later separation and purification of the fusion enzyme, the JaWa can remove peroxidation activity of the enzyme, the WamPa has good stability in an
organic solvent, and the SUMO tag can effectively improve the expression quantity of the enzyme, so that the reaction is efficiently catalyzed. In addition, after JaWa, SoLo and WamPa are fused with a 6 * His-SUMO
label, separation and purification can be carried out by using the
chelation of
histidine and
metal ions Ni < 2 + >, and the catalytic activity of the separated and purified enzyme is greatly improved.