Method for fast optical tracking of single molecule and device thereof

An optical tracking and single-molecule technology, which is applied in the detection and signal processing of single-molecule fluorescence spectroscopy, can solve problems such as long integration time, cover up the fine dynamic change process of single-molecule, and fail to meet the requirements of single-molecule trajectory measurement, and achieve suppression effect of influence

Active Publication Date: 2010-02-24
SHANXI UNIV
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Problems solved by technology

However, this method requires a long integration time, generally several minutes to tens of minutes, which will cover up the fine dynamic change

Method used

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  • Method for fast optical tracking of single molecule and device thereof
  • Method for fast optical tracking of single molecule and device thereof
  • Method for fast optical tracking of single molecule and device thereof

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Embodiment Construction

[0019] The following specific embodiments take SRfluor dye fluorescent molecules as an example to describe the method and device of the present invention in detail. The molecular structural formula of SRfluor dye is:

[0020]

[0021] Its absorption peak is 640nm, and its fluorescence emission peak is 670nm. Dye fluorescent molecules are dissolved and diluted to 10 -9 mol / mol and then placed in glycerol solution. Drop the mixed solution on the center of the cleaned coverslip, and the dye fluorescent molecules are randomly distributed in the solution, approximately every 10 μm 2 There is a dye molecule in .

[0022] The present invention can adopt multiple known instruments to realize, and the instrument adopted in the specific embodiment is: 1-pulse laser (PicoQuant, PDL808), 2MHz, the laser wavelength of output is 633nm, and power density is 1.5kW / cm 2 ; 2-Acousto-optic modulator (Crystal Technology, 3080-122); 3-Single photon detector (SPCM-15); 4-Single photon counti...

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Abstract

The invention discloses a method for fast optical tracking of a single molecule and a device, and relates to a technology for detecting single-molecule fluorescence spectroscopy and signal processing.The method uses the single-photon detection technology for detecting molecular fluorescence, analyzes the detected position with strongest fluorescence strength for determining the position of the single molecule, controls a nano-translation stage by feedback of a computer for tracking the position of the molecule and realizes the fast tracking of the motion trajectory of the molecule. The methodadopts the low-frequency sine wave modulation for triggering a laser signal of a single-molecule sample, thereby improving the signal-to-noise ratio. Compared with the prior art, the method adopts the single-photon detection technology, thereby being capable of determining and fast tracking the position of the single molecule in a relatively fine manner; the central position of the optoelectronicfeedback control nano-translation stage is changed along with the position of the single molecule, thereby being capable of reflecting the motion trajectory of the single molecule in a real-time manner; and the use of the modulation and demodulation technology can effectively suppresses the impacts of background noise on the detection of a fluorescence signal of the single molecule.

Description

technical field [0001] The invention relates to a single-molecule fluorescence spectrum detection and signal processing technology, in particular to a method and device for fast optical tracking of a single molecule. Background technique [0002] For many years, people's observation and research on the microcosm have been based on the average of multi-molecular ensembles. It was not until the emergence of scanning tunneling microscopes and optical tweezers in the 1980s, and the formation and development of single-molecule science in the 1990s that people observed and manipulated the microscopic material world at the atomic and molecular scales. Single-molecule detection can reveal the structure and motion state of biological macromolecules. For spatially distributed samples, such as a living cell, single-molecule detection can provide the precise position of the molecule under study, and can directly and non-interventionally observe trace amounts of biological substances in ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 肖连团贾锁堂王晓波魏志峰张国锋赵延霆
Owner SHANXI UNIV
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