Method for fast optical tracking of single molecule and device thereof
An optical tracking and single-molecule technology, which is applied in the detection and signal processing of single-molecule fluorescence spectroscopy, can solve problems such as long integration time, cover up the fine dynamic change process of single-molecule, and fail to meet the requirements of single-molecule trajectory measurement, and achieve suppression effect of influence
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[0019] The following specific embodiments take SRfluor dye fluorescent molecules as an example to describe the method and device of the present invention in detail. The molecular structural formula of SRfluor dye is:
[0020]
[0021] Its absorption peak is 640nm, and its fluorescence emission peak is 670nm. Dye fluorescent molecules are dissolved and diluted to 10 -9 mol / mol and then placed in glycerol solution. Drop the mixed solution on the center of the cleaned coverslip, and the dye fluorescent molecules are randomly distributed in the solution, approximately every 10 μm 2 There is a dye molecule in .
[0022] The present invention can adopt multiple known instruments to realize, and the instrument adopted in the specific embodiment is: 1-pulse laser (PicoQuant, PDL808), 2MHz, the laser wavelength of output is 633nm, and power density is 1.5kW / cm 2 ; 2-Acousto-optic modulator (Crystal Technology, 3080-122); 3-Single photon detector (SPCM-15); 4-Single photon counti...
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