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Method for quantitatively detecting salbutamol by using CdTe quantum dots

A technology for quantitative detection of salbutamol, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of high analysis cost, long detection period, cumbersome detection process, etc., and achieve simple experimental operation, short detection time, and wide linear range. Effect

Active Publication Date: 2015-07-22
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention is that when using liquid chromatography tandem mass spectrometry to detect β-receptor agonists in livestock products in the prior art, there are complicated sample pretreatment, cumbersome detection process, high analysis cost, long detection cycle, and expensive instruments. , difficult to operate and other problems, thus proposing a CdTe fluorescent sensor with simple operation, low cost and rapid determination of salbutamol and its preparation method and application

Method used

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  • Method for quantitatively detecting salbutamol by using CdTe quantum dots
  • Method for quantitatively detecting salbutamol by using CdTe quantum dots
  • Method for quantitatively detecting salbutamol by using CdTe quantum dots

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Embodiment 1

[0047] This embodiment provides a method for quantitatively detecting salbutamol in pig urine sample liquid by using CdTe quantum dots. The pig urine sample liquid is a negative sample. It is known that the negative pig urine sample liquid does not contain salbutamol, and it is used as a blind sample For detection, the detection method comprises the steps:

[0048] (a) the preparation of described CdTe quantum dots, specifically as follows:

[0049] (1) Weigh 0.1276g of tellurium powder and 0.0800g of sodium borohydride respectively, add 1ml of high-purity water, quickly seal the seal with a parafilm, insert the injection needle to communicate with the outside world at the same time, and react for 4 hours in an ice-water bath and strong magnetic stirring conditions , let it stand for 10 minutes to obtain a slightly pink and transparent NaHTe solution;

[0050] The reaction equation is as follows:

[0051] 4NaBH 4 +2Te+7H 2 O=2NaHTe+Na 2 B 4 o 7 +14H 2 ↑

[0052] (2) W...

Embodiment 2

[0064]The present embodiment provides a method for quantitatively detecting albuterol in swine urine sample liquid by using CdTe quantum dots, and the detection method comprises the following steps:

[0065] (a) The preparation of CdTe quantum dots is as follows:

[0066] (1) Weigh 0.12g of tellurium powder and 0.0900g of sodium borohydride respectively, add 2ml of high-purity water, quickly seal the seal with a parafilm, insert the injection needle to communicate with the outside world at the same time, and react for 4 hours in an ice-water bath and strong magnetic stirring conditions , let it stand for 10 minutes to obtain a slightly pink and transparent NaHTe solution;

[0067] (2) Weigh 0.1gCdCl 2 2.5H 2 Dissolve O in 200ml high-purity water, then add 100μL thioglycolic acid to form a milky white suspension system;

[0068] (3) First use 0.1mol L -1 NaOH solution adjusts the pH of the suspension system to be 10, passes through high-purity nitrogen for 30 min, then adds...

Embodiment 3

[0075] The present embodiment provides a method for quantitatively detecting albuterol in swine urine sample liquid by using CdTe quantum dots, and the detection method comprises the following steps:

[0076] (a) The preparation of CdTe quantum dots is as follows:

[0077] (1) Weigh 0.13g tellurium powder and 0.0700g sodium borohydride respectively, add 1.5ml high-purity water, quickly seal the seal with a parafilm, insert the injection needle to communicate with the outside world at the same time, carry out the reaction under the conditions of ice-water bath and strong magnetic stirring After 4 hours, let it stand for 10 minutes to obtain a slightly pink and transparent NaHTe solution;

[0078] (2) Weigh 0.2gCdCl 2 2.5H 2 Dissolve O in 200ml high-purity water, then add 100μL thioglycolic acid to form a milky white suspension system;

[0079] (3) First use 0.1mol L -1 NaOH solution adjusts the pH of the suspension system to be 11, passes high-purity nitrogen gas for 30 min...

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Abstract

The invention provides a method for quantitatively detecting salbutamol by using CdTe quantum dots. The method comprises the following steps: uniformly mixing the CdTe quantum dots with surfaces modified by mercaptoacetic acid with a substance to be detected, namely salbutamol, in a phosphate buffer solution, and then detecting fluorescent strength of a system by adopting a molecular fluorometer to realize trace detection of salbutamol because that in a weakly alkaline solution system, the CdTe quantum dots with the surfaces modified with carboxyl groups having negative charges and target molecules having positive charges, namely salbutamol, form a new compound system through electrostatic interaction so as to cause fluorescence quenching. Detection results show that salbutamol has relatively high sensitivity and a relatively wide linear range against the fluorescence quenching of the CeTe quantum dots, so that the changes in the fluorescent strength of the CdTe quantum dots are used for realizing the quantitative detection of salbutamol; and furthermore, the method for quantitatively detecting salbutamol by using the CdTe quantum dots, provided by the invention, is very simple in experimental operation, the detection time is short and the analysis cost is low.

Description

technical field [0001] The invention relates to a method for quantitatively detecting salbutamol by using CdTe quantum dots, and belongs to the detection field of salbutamol. Background technique [0002] Salbutamol is a β-receptor agonist, which can make the nutrients in the animal body transfer from fat to muscle, showing a nutrient redistribution effect, and then regulate the material metabolism of the animal body, enhance fat decomposition, promote protein synthesis, and significantly improve carcass lean meat. rate and feed remuneration. However, β-receptor agonists tend to accumulate residues in animal tissues, especially viscera. After entering the human body through the food chain, they can cause symptoms such as muscle tremors, arrhythmia, and headaches. In severe cases, they may be life-threatening. In view of the above reasons, my country has listed albuterol, clenbuterol, ractopamine and other β-receptor agonists as drugs that are prohibited from being used in f...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 张金艳魏益华罗林广邱素艳廖且根胡丽芳魏本华
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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