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Electrochemical sensor electrode surface nano-material modification method and sensor

A sensor electrode and nanomaterial technology, which is applied in the direction of material analysis, scientific instruments, and analytical materials by electromagnetic means, can solve the lack of effective solutions for rapid, sensitive and specific detection of cancer, and cannot solve the problem of rapid, sensitive, and multi-parameter analysis of trace cancer proteins. Quantitative detection and other issues, to achieve the effect of universal promotion and application value, low cost, and high sensitivity

Inactive Publication Date: 2018-12-21
SHANDONG NORMAL UNIV
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Problems solved by technology

[0009] In summary, the current technology cannot solve technical problems such as rapid and sensitive multi-parameter quantitative detection of trace cancer proteins or polypeptide molecules, and there is still a lack of effective solutions for rapid, sensitive and specific detection of cancer.

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  • Electrochemical sensor electrode surface nano-material modification method and sensor

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Embodiment Construction

[0031] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0032] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0033] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinatio...

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Abstract

The invention discloses an electrochemical sensor electrode surface nano-material modification method and a sensor. Carbon paper is used as a working electrode substrate; graphene nano, thionine and nano gold are modified on the surface of a working electrode to form a composite biosensitive membrane; and a specific antibody is fixed to the biosensitive membrane, and antigen detection is realizedby utilizing that the antigen concentration is proportional to the reduction degree of the reaction current. The multilayer nano sensitive membrane sensor can realize trace detection of high-sensitivity cancer protein and polypeptide molecules.

Description

technical field [0001] The invention relates to a nanometer material modification method on the electrode surface of an electrochemical sensor and a sensor. Background technique [0002] Lung cancer tumor markers (such as CEA, NSE, etc.), the detection of these protein and polypeptide cancer markers has important practical application value for early diagnosis and early intervention treatment of cancer (such as lung cancer tumors). Existing labeled immunoassay methods include enzyme-linked immunoassay (EL ISA), electrochemiluminescence immunoassay, radioimmunoassay, etc. However, enzyme-linked immunosorbent adsorption (EL ISA) or electrochemiluminescence immunoassays require pre-labeling of antigens or antibodies, which cannot meet the needs of instant and rapid detection of cancer protein or polypeptide molecules. [0003] At present, clinically, patients need to go to the hospital to collect blood for testing regularly, and there are problems such as long testing time and...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N33/574
CPCG01N27/3277G01N27/3278G01N33/57473G01N33/5748
Inventor 孙建辉
Owner SHANDONG NORMAL UNIV
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