Electrochemical sensor electrode surface nano-material modification method and sensor

A sensor electrode and nanomaterial technology, which is applied in the direction of material analysis, scientific instruments, and analytical materials by electromagnetic means, can solve the lack of effective solutions for rapid, sensitive and specific detection of cancer, and cannot solve the problem of rapid, sensitive, and multi-parameter analysis of trace cancer proteins. Quantitative detection and other issues, to achieve the effect of universal promotion and application value, low cost, and high sensitivity

Inactive Publication Date: 2018-12-21
SHANDONG NORMAL UNIV
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[0009] In summary, the current technology cannot solve technical problems such as rapid and sensitive multi-parameter quantitative detection of tra

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  • Electrochemical sensor electrode surface nano-material modification method and sensor

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[0031] The present invention will be further described below in conjunction with the drawings and embodiments.

[0032] It should be pointed out that the following detailed descriptions are all illustrative and are intended to provide further explanations for the application. Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.

[0033] It should be noted that the terms used here are only for describing specific implementations, and are not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should also be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate There are features, steps, operations, device...

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Abstract

The invention discloses an electrochemical sensor electrode surface nano-material modification method and a sensor. Carbon paper is used as a working electrode substrate; graphene nano, thionine and nano gold are modified on the surface of a working electrode to form a composite biosensitive membrane; and a specific antibody is fixed to the biosensitive membrane, and antigen detection is realizedby utilizing that the antigen concentration is proportional to the reduction degree of the reaction current. The multilayer nano sensitive membrane sensor can realize trace detection of high-sensitivity cancer protein and polypeptide molecules.

Description

technical field [0001] The invention relates to a nanometer material modification method on the electrode surface of an electrochemical sensor and a sensor. Background technique [0002] Lung cancer tumor markers (such as CEA, NSE, etc.), the detection of these protein and polypeptide cancer markers has important practical application value for early diagnosis and early intervention treatment of cancer (such as lung cancer tumors). Existing labeled immunoassay methods include enzyme-linked immunoassay (EL ISA), electrochemiluminescence immunoassay, radioimmunoassay, etc. However, enzyme-linked immunosorbent adsorption (EL ISA) or electrochemiluminescence immunoassays require pre-labeling of antigens or antibodies, which cannot meet the needs of instant and rapid detection of cancer protein or polypeptide molecules. [0003] At present, clinically, patients need to go to the hospital to collect blood for testing regularly, and there are problems such as long testing time and...

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IPC IPC(8): G01N27/327G01N33/574
CPCG01N27/3277G01N27/3278G01N33/57473G01N33/5748
Inventor 孙建辉
Owner SHANDONG NORMAL UNIV
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