Double-color ratio type quantum dot aerogel microsensor for glucose and application thereof

A technology of micro-sensors and glucose, applied in chemical instruments and methods, fluorescence/phosphorescence, inorganic chemistry, etc., can solve the problems of single-emission quantum dot fluorescence intensity artificial operation and environmental impact, and achieve quantitative analysis, high quantitative Analytical, high-sensitivity effects

Active Publication Date: 2019-11-22
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the fluorescence intensity of single-emission quantum dots is easily affected by human operation and the environment, especially for visual detection, its fluorescence is i

Method used

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  • Double-color ratio type quantum dot aerogel microsensor for glucose and application thereof
  • Double-color ratio type quantum dot aerogel microsensor for glucose and application thereof
  • Double-color ratio type quantum dot aerogel microsensor for glucose and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Such as figure 1 with figure 2 A two-color ratiometric C / CdTe-GOx airgel microsensor for glucose is shown, including a microfluidic chip body, and the microfluidic chip includes a chip upper layer 100, a chip matrix substrate 300, a detection chamber 201, and a fluid inlet 101 and the fluid outlet 102, the fluid inlet 101 and the fluid outlet 102 are arranged on the chip layer 100, the detection chamber 201 is arranged between the chip layer 100 and the chip matrix substrate layer 300, and the two ends of the detection chamber 201 are connected to the fluid inlet 101 and the fluid outlet respectively. 102 are connected; the detection cavity 201 is provided with quantum dot airgel for detecting glucose; the material of the chip layer 100 is polydimethylsiloxane, UV resin or polymethyl methacrylate; the matrix substrate material is glass, silicon sheet or quartz; the chip layer 100 and the host substrate layer 300 are bonded by irreversible plasma oxidation or polymer m...

Embodiment 2

[0041] Application of the microsensor prepared in Example 2 in the visual qualitative detection of glucose

[0042] Mix glucose with PBS buffer to make 0mM, 4.3mM, 6.3mM, 8.7mM, 10.8mM, 13mM, 26mM glucose solutions, and inject 1.4μL of glucose solutions of various concentrations into the detection chamber through a microsampler After 5 minutes, use a 365nm ultraviolet lamp to irradiate the chip to observe its fluorescent color. According to the concentration of the glucose solution, the fluorescent color emitted by the chip is different, such as Figure 4 shown.

Embodiment 3

[0043] Application of the microsensor prepared in Example 3 in the quantitative detection of glucose

[0044] Mix glucose with PBS buffer to make 0mM, 4.3mM, 6.3mM, 8.7mM, 10.8mM, 13mM, 26mM glucose solutions, and inject 1.4μL of glucose solutions of various concentrations into the detection chamber through a microsampler After 5 minutes, irradiate the chip with a 365nm ultraviolet lamp and take its fluorescence image, read the RGB value of the fluorescence image and calculate its average R / G value, take the R / G value as the ordinate, and the glucose concentration C as the abscissa, and use origin9 to simulate get along like Figure 5 The microsensor detects glucose working curve.

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Abstract

The invention discloses a double-color ratio type quantum dot aerogel microsensor for glucose. The sensor comprises a micro-fluidic chip body with a fluid inlet and a fluid outlet, wherein a detectioncavity connected with the fluid inlet and the fluid outlet is further formed in the chip body, and a double-color ratio type quantum dot aerogel structure capable of being used for detecting glucoseis synthesized in the detection cavity, the aerogel structure can reflect different fluorescence colors aiming at glucose solutions with different contents, so people can obtain the glucose content ofthe detected solution through naked eye and instrument analysis. The invention also discloses an application of the microsensor in visual qualitative detection and quantitative detection of the glucose. The detection device is portable, can realize qualitative and rapid visual analysis, and can also realize high-sensitivity quantitative analysis.

Description

technical field [0001] The invention relates to airgel sensor technology, in particular to a two-color ratio quantum dot airgel microsensor for glucose and its application. Background technique [0002] Fluorescent quantum dots are used as an ideal fluorescent material because their excited fluorescence has excellent spectral characteristics and stable photochemical properties, and its fluorescent color can be determined by its size without changing the surface composition. In the glucose photochemical enzyme biosensor. [0003] Quantum dot-based fluorescence detection methods can be divided into single emission type and ratiometric fluorescence type according to their signal output mode. The single-emission fluorescence detection method is a technique in which the fluorescence intensity of quantum dots with a single emission wavelength will increase or decrease due to the presence of the detection substance, and the quantitative detection of the detection substance can be ...

Claims

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Application Information

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IPC IPC(8): G01N21/64C01B19/00
CPCC01B19/007G01N21/64
Inventor 倪中华胡涛叶亦徐康恺
Owner SOUTHEAST UNIV
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