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Fluorescence detection method for acetamiprid

A fluorescence detection and acetamiprid technology, applied in the field of analytical chemistry detection, can solve the problems of cumbersome operation steps, long analysis cycle, low selectivity, etc., and achieve the effect of expanding application and high selectivity

Active Publication Date: 2018-06-05
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as cumbersome operation steps, low selectivity, and long analysis period.

Method used

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  • Fluorescence detection method for acetamiprid
  • Fluorescence detection method for acetamiprid
  • Fluorescence detection method for acetamiprid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A fluorescence detection method for acetamiprid, comprising the steps of:

[0036] (1) Preparation of fluorescent carbon quantum dots

[0037] Dissolve 1.5 g of citric acid in 15-20 ml of water, and stir magnetically for 5 minutes to dissolve all the solids; add 500 μl ethylenediamine with a pipette gun, and stir magnetically for 10 minutes to form a homogeneous solution; React at ℃ for 5 hours; transfer the solution to a pretreated dialysis bag (with a cut-off of 500) for dialysis for 36 hours; freeze-dry to obtain a viscous brown carbon quantum dot solid.

[0038] (2) Activation of carbon quantum dots and aptamer functionalization

[0039] Take 30ml of 3.5μg / ml carbon quantum dot solution and 60mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 25mg of N-hydroxysuccinimide (NHS) mixed, and shaken at room temperature for 15 minutes to obtain an activated carbon quantum dot solution; divide the activated carbon quantum dots into 2 parts on ave...

Embodiment 2

[0044] A fluorescence detection method for acetamiprid, comprising the steps of:

[0045] (1) Preparation of fluorescent carbon quantum dots

[0046] Dissolve 2.0 g of citric acid in 15-20 ml of water, stir magnetically for 5 minutes to dissolve all the solids; add 500-600 μl ethylenediamine with a pipette, and stir magnetically for 10 minutes to form a homogeneous solution; transfer the solution to a hydrothermal reaction kettle , react at 160-200°C for 5 hours; transfer the solution into a pretreated dialysis bag (with a cut-off of 500) and dialyze for 24-72 hours; freeze-dry to obtain viscous brown carbon quantum dot solids.

[0047] (2) Activation of carbon quantum dots and aptamer functionalization

[0048] Take 28ml of 4 μg / ml carbon quantum dot solution and 50mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 30 mg of N-hydroxysuccinyl Mix with amine (NHS), shake at room temperature for 15 minutes, and shake at room temperature for 15 minutes ...

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Abstract

The invention relates to a fluorescence detection method for acetamiprid. The fluorescence detection method comprises the following steps: (S1) activating carbon quantum dots, equally dividing the carbon quantum dots into two parts, adding one part into a nucleic acid aptamer solution with a specific recognition effect to acetamiprid, adding the other part into a nucleic acid aptamer complementarychain solution, carrying out oscillation coupling, and mixing two solutions, so as to obtain carbon quantum dot mixed liquid; (S2) separating residual nucleic acid aptamers and complementary chains in the carbon quantum dot mixed liquid obtained in the step (S1) by virtue of an electrophoresis method, so as to obtain a complementary chain modified carbon quantum dot solution only containing the aptamers; and (S3) adding a to-be-tested acetamiprid sample into the carbon quantum dot liquid obtained in the step (S1), oscillating for reaction, and measuring the fluorescence intensity of the solution by virtue of a molecular fluorescence photometer. By modifying the carbon quantum dots with the nucleic acid aptamers with a specific recognition effect to acetamiprid and the complementary chainof the aptamers, a rapid and sensitive detection method for acetamiprid is established through the change of the fluorescence intensity of the carbon quantum dots before and after acetamiprid is added.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry detection, in particular to a fluorescence detection method of acetamiprid. Background technique [0002] Carbon quantum dots are a new type of nanomaterials, which have the advantages of wide absorption spectrum, narrow and symmetrical emission spectrum, controllable emission wavelength, low photobleaching, high quantum yield, and large Stokes shift. Ideal fluorescent material. Based on its excellent photoelectric properties, carbon quantum dots have been deeply researched and applied in many fields, such as biological fields (cell imaging and animal living imaging), analytical fields (detection of metal and non-metal ions, small molecular compounds, etc.), Energy fields (carbon quantum dot sensitized solar cells, etc.) and optoelectronic devices, etc. [0003] Acetamiprid is a chlorinated nicotinic compound and is a new type of insecticide. The analysis method of acetamiprid in v...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/31C01B32/15C09K11/65B82Y20/00
CPCB82Y20/00C09K11/65G01N21/31G01N21/6428G01N2021/6432
Inventor 焦哲梁洪澳卢晓霞陈洪伟范洪波
Owner DONGGUAN UNIV OF TECH
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