Macromolecular fluorescent microspheres and preparation method thereof

A technology of fluorescent microspheres and polymers, which is applied in the field of preparation of fluorescent sensing materials, can solve the problems of poor solubility of conjugated polymers, difficulties in the adjustment and controllability of fluorescent properties, and achieve easy characterization, heterogeneous Simple and controllable effects

Inactive Publication Date: 2010-09-08
SUZHOU UNIV +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

It has been reported in the literature that fluorescent microspheres are prepared by adsorbing conjugated polymer electrolytes on the surface of microspheres by electrostatic adsorption, and its application in sensors is discussed (Langmuir2007, 23, 112-115). Although this method is relatively simple, it has There are also some problems, such as conjugated polymers must be well dissolved in a certain solvent before adsorption, however, the poor solubility of conjugated polymers is well known, even if the introduction of solubilizing groups in the side groups may not be able to In addition, the solubility of the conjugated polymer on the surface of the microsphere will bring about whether the conjugated polymer can exist stably on the surface of the microsphere, that is, the stability of the fluorescent performance of the microsphere
There are also documents disclosing that the surface of the microsphere is functionalized, reacted with a

Method used

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  • Macromolecular fluorescent microspheres and preparation method thereof
  • Macromolecular fluorescent microspheres and preparation method thereof
  • Macromolecular fluorescent microspheres and preparation method thereof

Examples

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Example Embodiment

[0022] Example one:

[0023] 1. Using Wessling's salt polymer precursor method: In a 50ml two-necked flask, protected with nitrogen, add 1.0g benzene ring with dodecyloxy side chain polymerized monomer and dissolve in 10ml tetrahydrofuran solution , Magnetically stir to make it completely dissolved, put it in an ice water bath, keep the reaction system at 0~5℃ under the protection of nitrogen, slowly add 2.4ml of 1.25M NaOH / MeOH solution, after reacting for 1 hour, add HCl to stop the reaction. The reaction solution is put into a dialysis bag with a molecular weight of 14000 and dialyzed in water for 24 hours to prepare a precursor of polymer PPV. The synthesis route is as follows:

[0024]

[0025] Among them, n is the number of repeating units.

[0026] See attached figure 1 , It is the nuclear magnetic map (HNMR) of the above-mentioned polystyrene (PPV) precursor, with deuterated chloroform as the solvent, at 7.0~6.7ppm, 4.5ppm, 3.9ppm, 2.9ppm, 1.9ppm and 0.9ppm, etc. It is the ...

Example Embodiment

[0030] Embodiment two:

[0031] 1. In a 50ml two-necked flask, protected with nitrogen, add 0.5g of polymer monomer without side groups, dissolve it in 10ml of water, magnetically stir to dissolve it completely, put it in an ice water bath, make the reaction system under nitrogen protection Keep 0~5℃, add 1.25M NaOH / H 2 1ml of O solution, after reacting for 1 hour, add HCl to terminate the reaction, put the reaction solution into a dialysis bag with a molecular weight of 14000 and dialyze in water for 24 hours to prepare the precursor of polymer PPV. The synthesis route is as follows:

[0032]

[0033] 2. Add 0.5 g of the precursor prepared in step 1 into an Erlenmeyer flask, dissolve it with 10 ml of water, and add 0.5 g of monodisperse polystyrene microspheres with a particle size of 25 microns on the surface, using 1.25M Adjust the pH value of NaOH to 9 and ultrasonically vibrate in an ultrasonic vibrator for 1 hour. After keeping the water temperature of the ultrasonic vibrator...

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Abstract

The invention discloses macromolecular fluorescent microspheres and a preparation method thereof. The macromolecular fluorescent microspheres use monodisperse sulfonated polystyrene microspheres as core microspheres, and the surfaces of the macromolecular fluorescent microspheres are coated with a shell layer of a p-phenylene vinylene (PPV) fluorescent conjugated macromolecular polymer. The preparation method of the macromolecular fluorescent microspheres comprises the following steps of: synthesizing a positively charged PPV fluorescent conjugated macromolecular polymer precursor in solution by using a Wessling sulfosalt polymer precursor method; adding the monodisperse sulfonated polystyrene microspheres into the precursor, and then processing the mixture for 0.5 to 2 hours under a vacuum condition that the temperature is between 90 and 150 DEG C; and washing and drying the mixture to obtain PPV conjugated macromolecular fluorescent microspheres. An inhomogeneous reaction for synthesizing the conjugated polymer in the method is simple and controllable, and the stability of the fluorescence of the microspheres is effectively improved.

Description

technical field [0001] The invention relates to a polymer fluorescent microsphere and a preparation method thereof, in particular to polystyrene fluorescent microspheres loaded with polystyrene (PPV) conjugated polymers on the surface and a preparation method thereof, belonging to the field of fluorescence sensing material preparation . Background technique [0002] Fluorescent microspheres refer to solid particles with diameters ranging from nanometers to micrometers, loaded with fluorescent substances, and excited by external energy to fluoresce. In recent years, the application of fluorescent microspheres in the field of biomedicine has attracted more and more attention. This is due to their stable shape, large specific surface area, strong adsorption, strong agglutination, strong surface reaction ability, and stable and efficient luminous efficiency. In addition, various properties such as biospecific binding, hollow porosity, magnetism, etc. can be endowed according to...

Claims

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Application Information

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IPC IPC(8): C08L25/06C08L25/18C09K11/06C09K11/02B01J13/02
Inventor 范丽娟王尚丽程丝王丽莎赵伟
Owner SUZHOU UNIV
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