Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection

A ratiometric fluorescent probe, copper nanocluster technology, applied in the field of fluorescence sensing, can solve the problems of high stability and difficulty in preparing extremely tiny particles, improve accuracy, eliminate fluctuations in excitation light intensity, and correct environmental interference Effect

Inactive Publication Date: 2015-07-01
NANCHANG UNIV
View PDF5 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, due to the difficulty in preparing highly stable and extremely small particles, the synthesis of CuNCs and the research on optical modulation are still in their infancy.
However, a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu&lt;2+&gt; detection
  • Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu&lt;2+&gt; detection
  • Preparation method of quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu&lt;2+&gt; detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Preparation of copper nanoclusters: 40 mg of polyethyleneimine was dissolved in 2 mL of ultrapure water, the pH of the solution was adjusted to 5 with acetic acid, and 2 mL of 2 mM Cu(NO 3 ) 2 solution to make Cu 2+ A complexation reaction with the amino ligand in polyethyleneimine occurred for 10 minutes, then 7 mg of ascorbic acid was added to the solution and stirred continuously for 8 hours, and the reaction solution was ultrafiltered at 16,000 rpm with an ultrafiltration tube with a molecular weight of 30 K In 10 minutes, copper nanoclusters are made;

[0020] (2) Preparation of quantum dot copper nanocluster ratiometric fluorescent probe: Mix copper nanocluster solution with 10 mM boric acid buffer solution and CdSe quantum dot solution, add 26.4 μg of 1-(3-dimethylaminopropyl)-3 -Ethylcarbodiimide hydrochloride (EDC), shaking reaction for 2 hours; use ultrafiltration tube with molecular weight of 30 K at 16000 rpm for 5 minutes, and the product is dispersed...

Embodiment 2

[0024] Ratiometric fluorescent probes for the detection of Cu 2+

[0025] (1) Optimization of pH, reaction temperature and reaction time

[0026] The pH, reaction temperature and reaction time were optimized. The results showed that, except for the strongly acidic environment (pH 2+ All have good responses, which may be due to the fact that the amino groups on the surface of CdSe QDsCuNCs are deprotonated under strong acidic conditions and cannot interact with Cu 2+ Complexation is carried out, and then the fluorescence quenching copper amine complex cannot be formed. However, the relative fluorescence intensity of CdSe QDsCuNCs (F 495 / F 625 ) slightly decreased under alkaline conditions, which may be due to part of the Cu 2+ Hydrolysis under alkaline conditions inhibits Cu 2+ Complexation reaction with hPEI-CuNCs. Temperature will affect Cu 2+ The complexation reaction with CdSe QDsCuNCs, the results of temperature optimization show that 37 o C is the optimum ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a preparation method of a quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection, belonging to the technical field of fluorescence sensing. According to the preparation method, CuNCs prepared by taking polyethyleneiminen as a template is coupled to the surface of silicon dioxide coated CdSe QDs to prepare CdSe QDs@CuNCs ratiometric fluorescent sensor having CdSe QDs and CuNCs double-fluorescence emission signals. When Cu<2+> exists in a solution, amino of polyethyleneimine and Cu<2+> are subjected to complexing to form a copper-amine complex, thereby avoiding the problem that CuNCs fluorescence quenching is caused but the fluorescence intensity of CdSe QDs is unchanged because electrons are transferred from amino to CuNCs. With the increase of Cu<2+> concentration, the intensity ratio of two fluorescence emission peaks of CdSe QDs and CuNCs is reduced gradually, thereby realizing sensitive fluorescent and visual detection on Cu<2+>.

Description

technical field [0001] The invention relates to a preparation method of a quantum dot copper nanocluster fluorescent probe and its Cu 2+ The detection application belongs to the technical field of fluorescence sensing. Background technique [0002] Copper in low concentration is an important and essential trace element in animals and plants, and it is also an important coenzyme factor of proteases in many organisms. However, when the concentration of copper ions is too high, it is highly toxic and can cause damage to the central nervous system, as well as some neurological diseases such as Wilson's disease and Alzheimer's disease. High concentrations of copper can induce gastrointestinal disturbances and damage to the liver or kidneys. To date, detection of Cu 2+ There are many methods, including atomic absorption / emission spectrometry, inductively coupled plasma mass spectrometry, electrochemical method, dynamic light scattering method, Raman scattering method and fluore...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C09K11/88G01N21/64
Inventor 邱建丁刘智超梁汝萍
Owner NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap