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A method of detecting the concentration of estrogen or estrogen-like compound

A technology of estrogen and compounds, applied in the direction of biological testing, material inspection products, etc., can solve the problems of affecting detection, lack of information and results, etc.

Active Publication Date: 2017-07-28
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High concentrations of estrogen receptor antagonists in some wastewaters are sufficient to affect the detection of estrogenic effects by biological assay methods, but this has received less attention in practical studies, with less relevant information and results

Method used

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  • A method of detecting the concentration of estrogen or estrogen-like compound
  • A method of detecting the concentration of estrogen or estrogen-like compound
  • A method of detecting the concentration of estrogen or estrogen-like compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1, the method for detecting and correcting the estrogen substance level in the chemical mixture sample

[0104] 1. Establishment of the concentration-estrogen effect relationship curve of the estrogen compound 17β-estradiol

[0105] (1) Insert the two-hybrid yeast into the SD medium and cultivate it until the two-hybrid yeast is in the logarithmic growth phase to obtain the initial bacterial liquid of the two-hybrid yeast, and use SD medium to adjust its absorbance at 600 nm to 0.75 (adjusted with SD medium Zero,), to obtain the two-hybrid yeast liquid.

[0106] (2) 17β-estradiol (E2) was dissolved in DMSO to make a concentration of 1×10 -10 ~1×10 -6 mol / L of the E2 standard test solution, the E2 standard test solution is added to the two-hybrid yeast solution in step (1) according to the volume ratio of 0.5% to prepare the exposure solution, so that the E2 concentration range in the exposure solution is 5×10 -13 ~5×10 -9 mol / L; Inoculate 200 μL / well of ...

Embodiment 2

[0167] Example 2, Detection and correction of estrogen compound and / or estrogen-like compound level in water sample of water source

[0168] Water samples collected from 16 water sources, covering six of the seven major river basins in my country, were used as samples of unknown types and concentrations of estrogen receptor disruptors, and the two-hybrid yeast method was used to test 16 water samples. Concentrations of similar estrogenic compounds and / or estrogen-like compounds were calculated and corrected for underestimation by estrogen receptor antagonists.

[0169] Specific steps are as follows:

[0170] 1. Use 24-hour continuous sampling technology to collect 20L of water samples from 16 water sources in China's six major river basins. Perform solid-phase extraction on each of the water samples to be tested and collect the eluate to realize the extraction of estrogen receptor interferents in each of the water samples to be tested, and then use DMSO to dilute each of the w...

Embodiment 3

[0202] Example 3. Detection and correction of the level of estrogen-like compounds in water samples from sewage treatment plants

[0203] The influent sample (A), the sand filter effluent sample (B), the first-line effluent sample (C) and the second-line effluent sample (D) collected from a sewage treatment plant in southern China were selected as samples for the types and concentrations of unknown estrogen receptor disruptors. Water samples were tested for estrogen-like compound concentrations using the two-hybrid yeast method and calculated for underestimation by estrogen receptor antagonists.

[0204] Specific steps are as follows:

[0205] 1. Collect 10L each of the influent sample (A), sand filter effluent sample (B), first-line effluent sample (C) and second-line effluent sample (D) of a sewage treatment plant in southern China. After performing solid phase extraction on each water sample to be tested, the eluate was collected, and then the eluate of each water sample t...

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Abstract

The invention discloses a method for detecting the concentration of estrogen or estrogen-like compounds. The invention discloses a method for detecting the concentration of a compound having an estrogenic effect in a sample, comprising the following steps: (1) establishing a concentration-estrogenic effect relationship curve of an estrogenic compound, comprising the following steps: A1, preparing a two-hybrid yeast in Two-hybrid yeast broth in logarithmic growth phase. The invention provides reliable technical means for toxicity detection and risk assessment of environmental mixed pollutants.

Description

technical field [0001] The invention relates to a method for detecting the concentration of estrogen or estrogen-like compounds, belonging to the field of biotechnology. Background technique [0002] Estrogen receptor disruptors are one of the hotspots in the research of endocrine disruptors. Estrogen receptor disruptors in the water environment include natural estrogens and other substances / antiestrogens derived from various sources. They can cause adverse effects on organisms at lower exposure concentrations, and joint exposure in the environment often means the possibility of addition or even interaction. It is therefore very important to monitor the environmental levels of estrogen receptor disruptors and their adverse effects on organisms. [0003] A number of agencies and organizations—including the U.S. Environmental Agency, the U.S. Department of Health and Human Services, the Organization for Economic Co-operation and Development, and others—have proposed lists of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74
Inventor 王子健杨蓉李娜饶凯锋马梅
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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