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Preparation method of quantum dot@copper nanocluster ratio fluorescent probe and its cu 2+ Detection application

A technology of ratiometric fluorescent probes and copper nanoclusters, which is applied in the field of fluorescent sensing, can solve the problems of high stability and difficulty in the preparation of extremely small particles, and achieve the effect of improving accuracy

Inactive Publication Date: 2016-08-17
NANCHANG UNIV
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  • Description
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  • Application Information

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Problems solved by technology

Therefore, due to the difficulty in preparing highly stable and extremely small particles, the synthesis of CuNCs and the research on optical modulation are still in their infancy.
However, a rapid and environmentally friendly method for synthesizing CuNCs and constructing ratiometric fluorescent probes for the detection of Cu at room temperature has not yet been seen. 2+ report

Method used

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  • Preparation method of quantum dot@copper nanocluster ratio fluorescent probe and its cu <sup>2+</sup> Detection application
  • Preparation method of quantum dot@copper nanocluster ratio fluorescent probe and its cu <sup>2+</sup> Detection application
  • Preparation method of quantum dot@copper nanocluster ratio fluorescent probe and its cu <sup>2+</sup> Detection application

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Embodiment 1

[0019] (1) Preparation of copper nanoclusters: 40 mg of polyethyleneimine was dissolved in 2 mL of ultrapure water, the pH of the solution was adjusted to 5 with acetic acid, and 2 mL of 2 mM Cu(NO 3 ) 2 solution to make Cu 2+ A complexation reaction with the amino ligand in polyethyleneimine occurred for 10 minutes, then 7 mg of ascorbic acid was added to the solution and stirred continuously for 8 hours, and the reaction solution was ultrafiltered at 16,000 rpm with an ultrafiltration tube with a molecular weight of 30 K In 10 minutes, copper nanoclusters are made;

[0020] (2) Preparation of quantum dots@copper nanocluster ratio fluorescent probe: Mix copper nanocluster solution with 10 mM boric acid buffer solution and CdSe quantum dot solution, add 26.4 μg of 1-(3-dimethylaminopropyl)- 3-Ethylcarbodiimide hydrochloride (EDC), shaking reaction for 2 hours; use ultrafiltration tube with a molecular weight of 30 K at 16000 rpm for 5 minutes, and the product is dispersed in...

Embodiment 2

[0024] Ratiometric fluorescent probes for the detection of Cu 2+

[0025] (1) Optimization of pH, reaction temperature and reaction time

[0026] The pH, reaction temperature and reaction time were optimized. The results showed that CdSeQDs@CuNCs had a positive effect on Cu in the pH range of 4-11 except for the strong acid environment (pH2+ All have good responses, which may be due to the fact that the amino groups on the surface of CdSe QDs@CuNCs are deprotonated under strong acidic conditions and cannot interact with Cu 2+ Complexation is carried out, and then the fluorescence quenching copper amine complex cannot be formed. However, the relative fluorescence intensity of CdSe QDs@CuNCs (F 495 / F 625 ) slightly decreased under alkaline conditions, which may be due to part of the Cu 2+ Hydrolysis under alkaline conditions inhibits Cu 2+ Complexation reaction with hPEI-CuNCs. Temperature will affect Cu 2+ The complexation reaction with CdSe QDs@CuNCs, the results of t...

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Abstract

The invention discloses a preparation method of a quantum dot@Cu nano-cluster ratiometric fluorescent sensor and application thereof in Cu<2+> detection, belonging to the technical field of fluorescence sensing. According to the preparation method, CuNCs prepared by taking polyethyleneiminen as a template is coupled to the surface of silicon dioxide coated CdSe QDs to prepare CdSe QDs@CuNCs ratiometric fluorescent sensor having CdSe QDs and CuNCs double-fluorescence emission signals. When Cu<2+> exists in a solution, amino of polyethyleneimine and Cu<2+> are subjected to complexing to form a copper-amine complex, thereby avoiding the problem that CuNCs fluorescence quenching is caused but the fluorescence intensity of CdSe QDs is unchanged because electrons are transferred from amino to CuNCs. With the increase of Cu<2+> concentration, the intensity ratio of two fluorescence emission peaks of CdSe QDs and CuNCs is reduced gradually, thereby realizing sensitive fluorescent and visual detection on Cu<2+>.

Description

technical field [0001] The invention relates to a preparation method of a quantum dot@copper nanocluster fluorescent probe and its Cu 2+ The detection application belongs to the technical field of fluorescence sensing. Background technique [0002] Copper in low concentration is an important and essential trace element in animals and plants, and it is also an important coenzyme factor of proteases in many organisms. However, when the concentration of copper ions is too high, it is highly toxic and can cause damage to the central nervous system, as well as some neurological diseases such as Wilson's disease and Alzheimer's disease. High concentrations of copper can induce gastrointestinal disturbances and damage to the liver or kidneys. To date, detection of Cu 2+ There are many methods, including atomic absorption / emission spectrometry, inductively coupled plasma mass spectrometry, electrochemical method, dynamic light scattering method, Raman scattering method and fluore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88G01N21/64
Inventor 邱建丁刘智超梁汝萍
Owner NANCHANG UNIV
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