Reagent for detecting brucella and complex probe fluorescence quantitative PCR (polymerase chain reaction) brucella detection method

Abstract

The invention provides a reagent for detecting brucella. The reagent comprises an upstream primer, a downstream primer, a fluorescence probe and a quenching probe; the gene sequence of the upstream primer is 5'-caagggcaaggtggaagatt-3'; the gene sequence of the downstream primer is 5'-ctgcgaccgatttgatgttt-3'; the gene sequence of the fluorescence probe is 5'-fam-atcgtttccgggtaaagcgtcgcca-P-3'; and the gene sequence of the quenching probe is 5'-cgctttacccggaaacga-Dabcyl-3'. The invention also provides a complex probe fluorescence quantitative polymerase chain reaction (PCR) brucella detection method using the reagent. The method is simple and convenient in operation, efficient, quick and specific; the detection time of the brucella is greatly shortened; the quantitative detection of a sample can be completed in about 2 hours; and the reagent and the method have significance for early diagnosis of brucella disease.

Description

technical field [0001] The invention relates to a reagent for detecting brucella and a detection method for brucella, in particular to a method for amplifying a composite probe brucella marker gene and then using fluorescent PCR technology to detect brucella. Background technique [0002] Brucellosis (brucellosis) is a zoonotic natural foci infectious disease caused by Brucella. Brucella can infect humans, a variety of domestic and wild animals, and cause fever, abortion and infertility, chronic arthritis, and neurological damage. After human beings are infected with brucellosis, due to the long course of the disease, it will cause huge economic losses and serious public health problems. In recent years, due to the gradual increase in livestock breeding and the increasingly frequent product circulation, brucellosis epidemics in humans and livestock have rebounded sharply in a considerable range. [0003] The most critical technical link in the prevention and control of inf...

AI Technical Summary

Problems solved by technology

These methods generally have shortcomings such as long detection time, cumbersome operation, poor sensitivity of the method, and many influencing factors, which cannot meet the needs of fast and accurate

The discovery of traditional PCR technology and nucleic acid hybridization technology has greatly promoted the development of bacterial detection technology, but there are defects such as easy cross-contamination and incapable of quantitative analysis.

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