In-situ composite system based on carbon quantum dot/manganese dioxide nanometer sheet layer and using method for detecting content of glutathione

A technology of glutathione content and carbon quantum dots, applied in the field of biological analysis, can solve the problems of high cost of gold and silver nanoclusters, unsuitable for large-scale promotion, destruction of biological samples, etc. High, adaptable effect

Active Publication Date: 2015-05-06
ZHENGZHOU UNIV
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  • Summary
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  • Description
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Problems solved by technology

However, when using a quantum dot detection system containing heavy metal ions, its biological safety and environmental friendliness need to be considered, and the cost of gold and silver nanoclusters is high, which is not suitable for large-scale promotion
Since upconversion nanoparticles need infrared light to be excited, the detection process generates more heat, which may cause damage to biological samples

Method used

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  • In-situ composite system based on carbon quantum dot/manganese dioxide nanometer sheet layer and using method for detecting content of glutathione
  • In-situ composite system based on carbon quantum dot/manganese dioxide nanometer sheet layer and using method for detecting content of glutathione
  • In-situ composite system based on carbon quantum dot/manganese dioxide nanometer sheet layer and using method for detecting content of glutathione

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Experimental program
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Effect test

Embodiment 1

[0030] Step 1, adding carbon quantum dots to the 2-(N-morpholine) ethanesulfonic acid buffer system to prepare a carbon quantum dot solution with a concentration of 0.1 mg / ml;

[0031] Step 2. Add 5 mL of 0.2 mM potassium permanganate aqueous solution to 60 μL of the above-mentioned carbon quantum dot solution. After fully mixing, add 3 mL of deionized water to the mixed solution to prepare a reaction mixture;

[0032] Step 3: Sonicate the above reaction mixture for 20 minutes until a brown precipitate is formed. After centrifugal purification, carbon quantum dots / manganese dioxide sheet nanocomposites are obtained, and then dispersed in 8 mL of deionized water to obtain carbon quantum dots. / Manganese dioxide sheet nanocomposite system.

[0033] Step 4. Use the in-situ composite system based on carbon quantum dots / manganese dioxide nanosheets obtained above as a detection solution, and combine it with glutathione at concentrations of 0 μM, 1 μM, 1.5 μM, 5 μM, 7.5 μM, and 10 μ...

Embodiment 2

[0036] Step 1, adding carbon quantum dots to the 2-(N-morpholine) ethanesulfonic acid buffer system to prepare a carbon quantum dot solution with a concentration of 0.5 mg / ml;

[0037] Step 2. Add 3 mL of 0.5 mM potassium permanganate aqueous solution to 18 μL of the above-mentioned carbon quantum dot solution, and after fully mixing, add 4.5 mL of deionized water to the mixed solution to prepare a reaction mixture;

[0038] Step 3. Ultrasonic the above reaction mixture for 25 minutes until a brown precipitate is formed. After centrifugal purification, carbon quantum dots / manganese dioxide lamellar nanocomposites are obtained, and then dispersed into 12mL deionized water to obtain carbon quantum dots. / Manganese dioxide sheet nanocomposite system.

[0039] Step 4. Use the in-situ composite system based on carbon quantum dots / manganese dioxide nanosheets obtained above as a detection solution, and combine it with glutathione at concentrations of 0 μM, 1 μM, 1.5 μM, 5 μM, 7.5 μM...

Embodiment 3

[0043] Step 1, adding carbon quantum dots to the 2-(N-morpholine) ethanesulfonic acid buffer system to prepare a carbon quantum dot solution with a concentration of 1.0 mg / ml;

[0044] Step 2. Add 2 mL of potassium permanganate aqueous solution with a concentration of 1.0 mM to 12 μL of the above-mentioned carbon quantum dot solution, and after fully mixing, add 6 mL of deionized water to the mixed solution to prepare a reaction mixture;

[0045] Step 3. Ultrasonic the above reaction mixture for 35 minutes until a brown precipitate is formed. After centrifugal purification, carbon quantum dots / manganese dioxide lamellar nanocomposites are obtained, and then dispersed into 16 mL of deionized water to obtain carbon quantum dots. / Manganese dioxide sheet nanocomposite system.

[0046] Step 4. Use the in-situ composite system based on carbon quantum dots / manganese dioxide nanosheets obtained above as a detection solution, and combine it with glutathione at concentrations of 0 μM, ...

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Abstract

The invention discloses an in-situ composite system based on a carbon quantum dot / manganese dioxide nanometer sheet layer and a using method of the in-situ composite system. The using method comprises the following steps: firstly, completely quenching fluorescence by using a manner that carbon quantum dots are adsorbed on a manganese dioxide nanometer sheet layer prepared by reducing potassium permanganate in situ, and then quantitatively analyzing and determining the content of the GSH in a GSH to-be-tested sample based on a principle of causing digestion of the manganese dioxide nanometer sheet layer, releasing the carbon quantum dots and recovering the fluorescence thereof by virtue of a redox reaction between glutathione (GSH) and manganese dioxide. According to the in-situ composite system, the detection method is high in precision, the repeatability is good, a used carbon material is low in price, easily available, and free from polluting the environment, and has relatively good biological safety; meanwhile, the in-situ composite system is simple and convenient to operate, relatively strong in practicability, high in sensitivity, and excellent in selectivity of the GSH.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, and specifically relates to an in-situ composite system based on carbon quantum dots / manganese dioxide nanosheets and a method for detecting glutathione content thereof. Background technique [0002] GSH includes glutamic acid, cysteine ​​and glycine, which are important peptide compounds in organisms and play a vital role in the metabolic process. The content of GSH in cells is closely related to many human diseases such as AIDS, Parkinson's syndrome, diabetes, Alzheimer's disease, cardiovascular disease and so on. Therefore, the detection of GSH content has become a research hotspot in recent years. [0003] At present, the commonly used detection methods of GSH content include high performance liquid chromatography (HPLC), surface enhanced Raman spectroscopy (SERS), capillary electrophoresis, enzyme-linked immunosorbent assay (ELISA) and so on. These detection methods are cumbers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 张琳李朝辉屈凌波葛佳蔡奇勇黄钟明
Owner ZHENGZHOU UNIV
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