Separation method for soil antagonistic bacteria

A separation method and antibacterial technology, applied in the field of microorganisms, can solve problems such as the inability to screen out bacteriostatic effects, and achieve the effects of improving separation success rate and separation efficiency, good market application prospects, and strong operability.

Inactive Publication Date: 2018-12-28
NINGDE NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a method for separating antagonistic bacteria in soil, which solves the problem that the existing method for separating antagonistic bacteria cannot screen out the antagonistic bacteria with the strongest antibacterial effect from a group of antagonistic bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for isolating soil antagonistic bacteria, comprising the following steps:

[0034] A: Prepare raw materials: collect a certain amount of soil samples from the main producing areas of Pseudostellaria heterophylla and tea. The soil samples are the soil 5cm below the surface, and prepare a certain amount of oat flour, agar and purified water;

[0035] B: Preparation of soil suspension: put the collected soil sample into a container (triangle flask) of appropriate size, then pour pure water into the container to moisten the soil until the soil is submerged, and shake for 5 minutes to fully diffuse the soil sample to obtain soil suspension;

[0036] C: Dilution: Absorb 1ml of the soil suspension with a sterile pipette, inject it into 9ml of sterile water, and repeat until the concentration is 10 -3 During the repeated process, a sterile pipette needs to be replaced every time it is repeated, and the tip of the pipette should not touch the liquid surface during oper...

Embodiment 2

[0043] A method for isolating soil antagonistic bacteria, comprising the following steps:

[0044] A: Prepare raw materials: collect a certain amount of soil samples from the main producing areas of Pseudostellaria heterophylla and tea, the soil samples are the soil 10cm below the surface, and prepare a certain amount of oat flour, agar and purified water;

[0045]B: Preparation of soil suspension: put the collected soil samples into a container of appropriate size (triangular flask), then pour pure water into the container to moisten the soil until the soil is submerged, shake for 6 minutes to fully diffuse the soil sample, and obtain soil suspension;

[0046] C: Dilution: Absorb 1ml of the soil suspension with a sterile pipette, inject it into 9ml of sterile water, and repeat until the concentration is 10 -4 During the repeated process, a sterile pipette needs to be replaced every time it is repeated, and the tip of the pipette should not touch the liquid surface during ope...

Embodiment 3

[0053] A method for isolating soil antagonistic bacteria, comprising the following steps:

[0054] A: Prepare raw materials: collect a certain amount of soil samples from the main producing areas of Pseudostellaria heterophylla and tea. The soil samples are the soil 15cm below the surface, and prepare a certain amount of oat flour, agar and purified water;

[0055] B: Preparation of soil suspension: put the collected soil samples into a container of appropriate size (triangle flask), then pour pure water into the container to moisten the soil until the soil is submerged, and shake for 7 minutes to fully diffuse the soil sample to obtain soil suspension;

[0056] C: Dilution: Absorb 1ml of the soil suspension with a sterile pipette, inject it into 9ml of sterile water, and repeat until the concentration is 10 -5 During the repeated process, a sterile pipette needs to be replaced every time it is repeated, and the tip of the pipette should not touch the liquid surface during op...

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Abstract

The invention discloses a separation method for soil antagonistic bacteria, and relates to the technical field of microorganisms. The separation method for the soil antagonistic bacteria comprises thefollowing steps: A, preparing raw materials, B, preparing a soil suspension, C, diluting, D, preparing an isolation medium, E, performing culture, F, carrying out selective separation, G, preparing an indicator bacteria plate, and H, screening. According to the invention, the antagonistic bacteria are separated from soil through a dilution separation method, actinomycetes in the medium are screened through adding phenol liquid to the medium, and the antagonistic bacteria with the strongest antibacterial effect in the medium are screened through an indicator bacteria plate confrontation method, and are developed into a biocontrol preparation for biocontrol of plant diseases, thereby having good market application prospects. The separation method for the soil antagonistic bacteria disclosedby the invention simplifies the steps of separating the antagonistic bacteria, can quickly separate the antagonistic bacteria from the soil, and improves the separation success rate and separation efficiency of the antagonistic bacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for isolating soil antagonistic bacteria. Background technique [0002] At present, the prevention and control of plant diseases is mainly based on chemical control. However, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and resistance of pathogenic bacteria. The characteristics of low residue, no pollution, not easy to produce drug resistance and easy availability of raw materials are recognized as ideal pharmaceuticals in the 21st century. [0003] Microbial pesticides are an important part of biological pesticides, mainly including microbial live pesticides and microbial metabolite pesticides. It has been more than half a century since the discovery and application of microbial pesticides. During this period, domestic and foreign experts have successively developed mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
CPCC12N1/02
Inventor 陈美霞叶祖云阮文元吴成加王泽榕
Owner NINGDE NORMAL UNIV
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