Bacillus subtilis JN005 and application thereof in prevention and control of rice blast

A technology of Bacillus subtilis and rice blast, applied in the direction of application, bacteria, and fungicides, can solve the problems of unsatisfactory effect, poor colonization ability of antagonistic microorganisms, and reduced control effect of antagonistic microorganisms, and achieve both protection and treatment. Tool, avoid adverse effects, good effect of prevention

Active Publication Date: 2015-10-14
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the field of biological control, there are often problems such as poor colonization ability of antagonistic microorganisms under natural conditions in the field and unstable effect on disease control.
A large number of studies have also shown that the antagonistic microorganisms screened indoors have a strong inhibitory effect on pathogenic bacteria in the greenhouse, but the effect in field control is not satisfactory. Colonize the root circle of plants, on the other hand, interact with the complex microbial species and antagonistic strains in the field soil, resulting in a decrease in the control effect of antagonistic microorganisms. Therefore, it is necessary to select and screen biological strains with strong field adaptability or high-efficiency metabolites Applying to field disease control is an important way to solve the above problems

Method used

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  • Bacillus subtilis JN005 and application thereof in prevention and control of rice blast
  • Bacillus subtilis JN005 and application thereof in prevention and control of rice blast
  • Bacillus subtilis JN005 and application thereof in prevention and control of rice blast

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1 Isolation of Bacillus subtilis JN005

[0025] Healthy rice plants were selected from the diseased clusters of blast-susceptible rice variety Xiangzaoxian No. 24 in Paixingshang Village (N28°31′49.02″, E111°54′24.86″), Daligang Town, Daligang Town, Taojiang County, Hunan Province, and 871 plants were isolated Strains, the plate confrontation method with Magnaporthe grisea was used to screen out an antagonistic antibacterial JN005 with an inhibition rate of 84% against Magnaporthe oryzae hyphae.

[0026] By cultivating strain JN005 (peptone 5g / L, beef extract 3g / L, glucose 20g / L, agar 17g / L, pH 7.0, prepared with distilled water) on NA solid medium ( figure 2 A and figure 2 B). Observe the morphology of the colony, observe the SEM image of the bacteria, and combine the physiological, biochemical, microbiological test results of the bacteria, and the 16S rDNA sequencing results (see SEQ ID NO. 1) to finally determine the strain JN005 is Bacillus subtilis (Bacillus s...

Embodiment 2

[0028] Example 2 Application of Bacillus subtilis JN005 in the control of rice blast

[0029] After the strain JN005 is activated on the NA solid medium plate, use a punch to punch 2 bacterial cakes with a diameter of 5 mm into 150mL NB liquid medium (peptone 5g / L, beef extract 3g / L, glucose 20g / L, pH 7.0, prepared with distilled water) in a 250 mL Erlenmeyer flask, shake culture in a shaker at 28°C and 180 r / min for 6 days, and filter with a bacterial filter to obtain the metabolites of Bacillus subtilis JN005.

[0030] Add 10 mL of the metabolites of strain JN005 and 0.1 mL of the conidia of Magnaporthe grisea (strain 81) into a sterilized Erlenmeyer flask, mix well, replace the antagonistic culture medium with sterile water as a control, cultivate in the dark at 27°C for 6 hours The germination of spores was checked under microscope, and the experiment was repeated 3 times. The results showed that the inhibition rate of JN005 metabolites on rice blast fungus spore germination ...

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Abstract

The invention discloses bacillus subtilis JN005 and application thereof in prevention and control of rice blast. The preservation number of the strain JN005 is CCTCC No: M2015228. According to the invention, the strain JN005 is utilized to prevent and control the rice blast, so as to avoid potential bad influence of chemical prevention and control on environment and rice safety and disease risk of disease-resistant variety utilization. The strain JN005 living bacteria and metabolite thereof have good prevention and control effect on rice blast and have protection and control effects, and can be developed to dosage forms of the living bactericide and metabolite thereof. In addition, the strain JN005 has inhibition effect on multiple pathogeny indicator bacteria.

Description

Technical field [0001] The invention relates to the technical field of biological control, in particular to a Bacillus subtilis JN005 and its application in the prevention and treatment of rice blast. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops. More than half of the world's population feed on rice. Rice blast (the pathogen is Magnaporthe oryzae and its asexual generation is Pyicularia oryzae) is one of the most serious fungal diseases of rice. Magnaporthe grisea (M.oryzae) is a semi-living parasitic, which has an obvious live vegetative stage at the initial stage of infection. Rice blast disease can be divided into seedling blast, leaf blast, knot blast, ear blast, grain blast, branch blast, etc. according to its location and time. The current production mainly uses disease-resistant varieties and chemical fungicides to control rice blast. The main fungicides used are tricyclazole, pyraclostrobin, prochloraz, pyraclamide, isomapyr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/125
CPCA01N63/00A01N63/10C12N1/205C12R2001/125
Inventor 刘二明任佐华管玲莉刘敏捷周鑫钰
Owner HUNAN AGRICULTURAL UNIV
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