Method for exploring indicator bacteria by utilizing n-butyl alcohol oxidizing bacteria as oil-gas microorganism

A technology for microbial exploration and n-butanol, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low safety in the experimental process, long cultivation period, poor data accuracy, etc., and achieve simple experimental operation process , reduction of cultivation time, and improvement of safety

Inactive Publication Date: 2011-04-06
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0003] The purpose of the present invention is to establish a method with simple process, high safety and easy operation and control in order to solve the problems related to the long cultivation period, low safety of the experimental process and poor accuracy of the obtained data in the experimental method used in the current oil and gas microbial exploration indicator bacteria test. A method of using n-butanol oxidizing bacteria as indicator bacteria for oil and gas microbial exploration

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Embodiment Construction

[0019] Microbiological prospecting technology indicator bacteria testing method of the present invention is made up of the following steps:

[0020] 1. Use the following medium formula to prepare the medium used for the oil and gas microbial exploration technology indicator bacteria test.

[0021] K H 2 PO 4 0.26g / L; Na 2 HPO 4 0.74g / L; MgSO 4 ·7H 2 O 1.0g / L; KNO 3 1.0g / L; NH 4 Cl0.5g / L; CaCl 2 2H 2 O 0.02g / L; NaCl 2g / L; n-butanol 10g / L; agar 20g / L.

[0022] 2. After air-drying the sample retrieved from the site, weigh 5g sample and dissolve it in 45mL distilled water, then dilute step by step to a certain extent, and shake evenly.

[0023] 3. In the aseptic room, according to the coating method, the sample after successive dilution is coated on the prepared medium plate, and placed in a 30°C incubator for cultivation.

[0024] 4. Count and record the data after placing and culturing for 5 days.

[0025] 5. Analysis method:

[0026] ① Determination of sampling ...

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Abstract

The invention relates to a method for exploring indicator bacteria by utilizing n-butyl alcohol oxidizing bacteria as oil-gas microorganism, comprising the following steps of: carrying out block process on a soil sample; coating the soil sample in the hydrocarbon oxidizing bacteria medium by utilizing a spread-plate method in an aseptic operating room; and putting the hydrocarbon oxidizing bacteria medium in a static incubator to cultivate the hydrocarbon oxidizing bacteria, wherein the n-butyl alcohol is used as the unique carbon and energy source of hydrocarbon oxidizing bacteria medium, the cultivating parameter is set as 30 DEG C, 5 days form a cultivating period, and the needed soil sample is air dried rapidly, then dissolved in distilled water and put in a shaking table to activate for use. The method has the advantages of shortening the cultivating period to 5 days, simplifying the whole experiment operating process, enhancing the safety, accurately obtaining the quantity of the needed objective colony by utilizing the medium with n-butyl alcohol as the unique carbon source, and enhancing the reliability of the colony count data through reducing the cultivating time.

Description

1. Technical field: [0001] The invention relates to a microbial oil and gas exploration technology, in particular to a method for using n-butanol oxidizing bacteria as indicator bacteria for oil and gas microbial exploration. 2. Background technology: [0002] At present, oil and gas microbial exploration technology has been successfully applied in unconventional oil and gas reservoirs and deep oil and gas reservoirs. Microbial oil and gas exploration technology mainly consists of three parts, namely: (1) determine the sampling points in the exploration area and collect samples; (2) test and analyze the indicator bacteria on the retrieved samples; (3) predict the distribution of oil and gas reservoirs through data analysis. The determination of the number of indicator bacteria is the core content of oil and gas microbial exploration technology, and this data is the core analysis index reflecting whether there are oil and gas reservoirs in the test block. Therefore, establis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/64C12Q1/02
Inventor 宋永亭宋欣汪卫东蒋焱高光军王静李彩风郭辽原
Owner CHINA PETROLEUM & CHEM CORP
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