Method for exploring indicator bacteria by utilizing n-butyl alcohol oxidizing bacteria as oil-gas microorganism
A technology for microbial exploration and n-butanol, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of low safety in the experimental process, long cultivation period, poor data accuracy, etc., and achieve simple experimental operation process , reduction of cultivation time, and improvement of safety
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[0019] Microbiological prospecting technology indicator bacteria testing method of the present invention is made up of the following steps:
[0020] 1. Use the following medium formula to prepare the medium used for the oil and gas microbial exploration technology indicator bacteria test.
[0021] K H 2 PO 4 0.26g / L; Na 2 HPO 4 0.74g / L; MgSO 4 ·7H 2 O 1.0g / L; KNO 3 1.0g / L; NH 4 Cl0.5g / L; CaCl 2 2H 2 O 0.02g / L; NaCl 2g / L; n-butanol 10g / L; agar 20g / L.
[0022] 2. After air-drying the sample retrieved from the site, weigh 5g sample and dissolve it in 45mL distilled water, then dilute step by step to a certain extent, and shake evenly.
[0023] 3. In the aseptic room, according to the coating method, the sample after successive dilution is coated on the prepared medium plate, and placed in a 30°C incubator for cultivation.
[0024] 4. Count and record the data after placing and culturing for 5 days.
[0025] 5. Analysis method:
[0026] ① Determination of sampling ...
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