Atmospheric steam sterilizing biological indicator and preparation method thereof

A technology of biological indicators and atmospheric pressure steam, which is applied in the direction of biochemical equipment and methods, and microbial measurement/inspection, which can solve the difference in the ability to indicate sterilization, reduce the accuracy of judgment results, and increase the difficulty of judging the sterilization effect And other issues

Inactive Publication Date: 2011-05-04
HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the dry spores of the indicator bacteria that encounter water in advance will absorb water and swell and turn to the growth state to reduce the resistance to atmospheric pressure steam. The spores that encounter water later still maintain strong resistance. Due to the different physiological states of the indicator bacteria spores As a result, there are great differences in the ability of the indicators in the same sterilizer to indicate sterilization, which increases the difficulty of judging the sterilization effect on the one hand, and reduces the accuracy of the judgment results on the other hand.

Method used

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  • Atmospheric steam sterilizing biological indicator and preparation method thereof
  • Atmospheric steam sterilizing biological indicator and preparation method thereof
  • Atmospheric steam sterilizing biological indicator and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 The indicator of the present invention was prepared by using Cottonseed Husk Thermoresistant Bacteria No. 01 as the indicator bacterium.

[0023] The indicator is composed of cottonseed hull antipyrogenic bacteria No. 01 waterproof indicator bacteria sheet and its dry medium (after adding water, it is used for the recovery growth of cottonseed hull antipyrogenic bacteria No. 01 indicator bacteria, which is in a sterile state). Any one of paraffin wax, stearic acid, palmitic acid, and paraffin wax emulsion can be used as a waterproof material for making waterproof indicator bacteria sheets.

[0024] (1) Preparation of waterproof indicator bacteria sheet:

[0025] (1) Preparation of indicator bacteria spore suspension

[0026] Streak-inoculate the cottonseed shell heat-resistant bacteria No. 01 indicator bacteria on the inclined surface of the spore-forming bacteria medium test tube, cultivate at 34-36 °C for 48 hours, and at 20 °C for 72 hours; wash off the ba...

Embodiment 2

[0062]Example 2 The indicator of the present invention was prepared by using the indicator bacteria screened from the corncob cultivation material and having the effect of indicating the normal pressure sterilization of the corncob cultivation material.

[0063] The indicator is composed of a corn cob cultivation material atmospheric pressure sterilization indicator bacteria sheet and its dry medium (after adding water, it is used for the recovery growth of the corn cob cultivation material normal pressure sterilization indicator bacteria, and is in a sterile state). Any one of paraffin wax, stearic acid, palmitic acid, and paraffin wax emulsion can be used as a waterproof material for making waterproof indicator bacteria sheets.

[0064] (1) Screening indicator bacteria for normal pressure sterilization of corncobs:

[0065] (1) Preparation of cultivation materials Weigh corncobs and bran according to the mass ratio of 7:3, mix them evenly, dry them in an oven at 35°C, crush ...

Embodiment 3

[0116] Example 3 Comparison of the indication effect when adding water between the indicator of the present invention and the conventional indicator

[0117] (1) Experimental materials

[0118] The indicator of the present invention is made with the indicator made in Example 1 (wherein the waterproof indicator bacteria sheet is made with paraffin emulsion), and the concentration of spores is 4 × 10 6 Each per milliliter, the amount of the culture medium is equivalent to 40 milliliters of the standard formula of the culture medium (40 milliliters of water is added when used), and it is divided into 100 milliliters of infusion bottles. The code name of the experiment is "Ben".

[0119] The conventional indicator is composed of cottonseed hull heat-resistant bacteria No. 01 spore bran agent and dry medium (in order to reduce errors, no color enhancer is added), and the concentration of spores is 4×10 6 Each per milliliter, the amount of the culture medium is equivalent to 40 mi...

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Abstract

The invention discloses an atmospheric steam sterilizing biological indicator and a preparation method thereof. The atmospheric steam sterilizing biological indicator comprises a dry culture medium and an indicator bacteria carrier, wherein the indicator bacteria carrier is formed by adhering the spore of the indicator bacteria onto a contaminated carrier sheet the surface of which is coated withparaffin, paraffin emulsion or fatty acid. The preparation method comprises the following steps: inoculating the indicator bacteria onto the inclined plane of a bacteria culture medium for generatingthe spore; after the spore is generated, preparing indicator bacteria suspension; adsorbing the indicator bacteria spore suspension, and dripping on the contaminated carrier sheet to prepare the contaminated carrier sheet; dipping the contaminated carrier sheet into melted paraffin or fatty acid liquid or paraffin emulsion; drying to obtain a waterproof indicator bacteria carrier; and combining the waterproof indicator bacteria carrier with the dry culture medium. When determining the atmospheric steam sterilizing effect, the indicator provided by the invention can ensure that time that the indicator bacteria spore is actually in contact with water is basically consistent no matter when water is added to an indicator bottle, thus effectively avoiding the problem that the indicator has different sterilizing abilities because the time when water is added to the indicator in the same autoclave is different.

Description

technical field [0001] The invention relates to an atmospheric pressure steam sterilization indicator and a preparation method of the indicator. Background technique [0002] Among the three physical, chemical and biological methods for monitoring the sterilization effect of edible fungus cultivation materials by atmospheric pressure steam, the biological monitoring method is the most reliable method. The principle of biological monitoring is to rely on a certain fungus that is resistant to atmospheric steam sterilization and has the ability to indicate the sterilization effect, culture its spores (spores) after sterilization, and determine whether the spores are alive or not. killing effect. The application of biomonitoring technology has indeed received significant effects in improving the sterilization effect, reducing the occurrence of pollution, and improving the growth of edible fungus mycelium. [0003] Atmospheric steam sterilization biological indicator is compose...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 王朝江袁永昌董庆武王世清高春燕
Owner HEBEI ACADEMY OF AGRI & FORESTRY SCI INST OF GENETICS & PHYSIOLOGY
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