Adipose tissue cryopreservation liquid at clinical application level and cryopreservation method

A fat tissue, clinically applied technology, applied in the field of biomedicine, can solve problems such as adverse reactions, achieve the effect of reducing the risk of cell DNA distortion and protein denaturation, avoiding side effects and reducing functional impact

Active Publication Date: 2019-06-07
陕西医赛尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the above problems, the present invention provides a clinically applied level of adipose tissue cryopreservation solution and cryopreservation method, which effectively avoids the use of 4-hydroxyethylpiperazineethanesulfonic acid (HEPES), phenol Red, DMSO, animal serum, platelet extracts, etc. also have the disadvantage of adverse reactions

Method used

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  • Adipose tissue cryopreservation liquid at clinical application level and cryopreservation method
  • Adipose tissue cryopreservation liquid at clinical application level and cryopreservation method
  • Adipose tissue cryopreservation liquid at clinical application level and cryopreservation method

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Experimental program
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Effect test

Embodiment 1

[0055] Such as Figure 1-Figure 6 As shown, clinical application grade adipose tissue cryopreservation solution, including:

[0056] 20mmol / L injectable grade sucrose;

[0057] 20mmol / L mannitol injection;

[0058] 5mmol / L glucose injection;

[0059] 2mol / L adenosine injection;

[0060] 3mmol / L reduced glutathione;

[0061] 1.2mol / L trehalose;

[0062] 0.3mmol / L of EDTA-2Na;

[0063] The injectable grade sucrose of described 20mmol / L, the mannitol injection of 20mmol / L, the glucose injection of 5mmol / L, the adenosine injection of 2mol / L, the reduced glutathione of 3mmol / L, 1.2mol Trehalose / L and EDTA-2Na of 0.3mmol / L are mixed to form a mixed solution, which is a clinical application level adipose tissue cryopreservation solution, and the solvent or diluent of the clinical application level adipose tissue cryopreservation solution Lactated Ringer's Injection. The component of described Sodium Lactated Ringer's Injection is: under the condition that the full amount of d...

Embodiment 2

[0078] Clinical application grade adipose tissue cryopreservation solution, including:

[0079] 50mmol / L injectable grade sucrose;

[0080] 50mmol / L mannitol injection;

[0081] 5mmol / L glucose injection;

[0082] 3mol / L adenosine injection;

[0083] 5mmol / L reduced glutathione;

[0084] 4.5mol / L trehalose;

[0085] 1mmol / L of EDTA-2Na;

[0086] The injectable grade sucrose of described 50mmol / L, the mannitol injection of 50mmol / L, the glucose injection of 5mmol / L, the adenosine injection of 3mol / L, the reduced glutathione of 5mmol / L, 4.5mol The trehalose / L and the EDTA-2Na of 1mmol / L are mixed to form a mixed solution, and this mixed solution is adipose tissue cryopreservation liquid of clinical application level, and the solvent or diluent of the adipose tissue cryopreservation liquid of described clinical application level is Sodium Lactated Ringer's Injection, the components of the Sodium Lactated Ringer's Injection are: under the condition that the total amount of t...

Embodiment 3

[0101] Clinical application grade adipose tissue cryopreservation solution, including:

[0102] 15mmol / L injectable grade sucrose;

[0103] 15mmol / L mannitol injection;

[0104] 1mmol / L glucose injection;

[0105] 1mol / L adenosine injection;

[0106] 0.5mmol / L reduced glutathione;

[0107] 1mol / L trehalose;

[0108] 0.1mmol / L of EDTA-2Na;

[0109] The injectable grade sucrose of described 15mmol / L, the mannitol injection of 15mmol / L, the glucose injection of 1mmol / L, the adenosine injection of 1mol / L, the reduced glutathione of 0.5mmol / L, 1mol Trehalose / L and EDTA-2Na of 0.1mmol / L are mixed to form a mixed solution, which is a clinical application level adipose tissue cryopreservation solution, and the solvent or diluent of the clinical application level adipose tissue cryopreservation solution Lactated Ringer's Injection. The component of described Sodium Lactated Ringer's Injection is: under the condition that the full amount of described Sodium Lactated Ringer's Inje...

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Abstract

Adipose tissue cryopreservation liquid at a clinical application level and a cryopreservation method. The adipose tissue cryopreservation liquid at the clinical application level comprises 15-50-mmol/L saccharose, 15-50-mmol/L mannitol injection, 1-5-mmol/L glucose injection, 1-3-mol/L adenosine injection, 0.5-5-mmol/L reduced glutathione, 1-4.5-mol/L trehalose and 0.1-1-mmol/L EDTA-2Na; and the components are mixed to form mixed liquid, and the mixed liquid is the adipose tissue cryopreservation liquid at the clinical application level. By means of the adipose tissue cryopreservation liquid,in combination with the cryopreservation method thereof, at the clinical application level, the defect of adverse reaction due to 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), phenol red, DMSO, animal serum, blood platelet extracts and the like which are used in the prior art is effectively overcome.

Description

technical field [0001] The invention relates to the field of biomedical technology, in particular to a clinical application level adipose tissue cryopreservation solution and a cryopreservation method, in particular to an adipose tissue cryopreservation solution and a cryopreservation method meeting clinical requirements. Background technique [0002] Adipose tissue is composed of a large number of clusters of fat cells, which are separated into lobules by a thin layer of loose connective tissue. The reticular fibers in adipose tissue are well developed. Adipose tissue has very little intercellular substance, and the fat in the joints of adipose tissue has the function of buffering muscle movement, and acts as a support pad in the arms and soles. [0003] In addition, with the continuous update of medical technology in recent years, the clinical application of adipose tissue as a natural filling material for the human body has attracted more and more attention, including so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张小波谢晓燕杨劲鹏
Owner 陕西医赛尔生物科技有限公司
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