The invention relates to the field of a medical apparatus, and discloses a closed type preparation device for antitumor medicines and a using method of the closed type preparation device. The closed type preparation device comprises a cylinder body and a sleeving cap, wherein flanges are arranged on the inner wall of the cylinder body, and are made from an elastic material; a connecting
pipe is fixed to the middle of the top of the sleeving cap, an injection needle coxially arranged at the end head close to the sleeving cap, of the connecting
pipe, and a needle point of the injection needle penetrates through the top cover of the sleeving cap and extends to the middle of the cylinder body; a pintle is arranged at the end head away from the sleeving cap, of the connecting
pipe; and the injection needle and the pintle communicate with an inner cavity of the connecting pipe. A
penicillin bottle is inserted into the cylinder body, the injection needle on the inner side of the sleeving
capis punctured into the
penicillin bottle, the flanges in the cylinder body are used for clamping the neck of the
penicillin bottle,
solvent liquid is injected to the penicillin bottle through the injection needle, and the cylinder body is held by the hands for shaking up medicament in the penicillin bottle, so that the condition that during the process, the liquid in the penicillin bottle can splash to air, can be avoided, the condition that medicines damage mucosa or
skin of medical personnel can be avoided, and generation of needling wound can be avoided.