The invention discloses a portable insulin refrigerating box for a diabetic patient. The freezing box comprises a box body, the top of the box body is rotationally connected with a sealing cover, thebox body is divided into a placing chamber and a cooling chamber by placing an inner box in the box body, and a fixing frame for fixing an insulin pen is arranged in the placing chamber. Since the equipment is provided with the fixing frame, when the user places the insulin pen, the user can directly insert the pen into a cooling liquid storage bag, the whole pen body is wrapped by low-temperaturecooling liquid, and therefore the cooling efficiency of the equipment is improved. Meanwhile, when the pen body is inserted into the bag, an auxiliary fixing assembly is squeezed, therefore, one-timefixing is achieved, later, once the freezing box is vibrated, a placing outer barrel is screwed upwards so as to squeeze an inflation air bag to inflate a limiting air bag, therefore, the pen body isfurther fixed, even though the refrigerating box is vibrated, the insulin pen in the box will not collide with the inner box, the insulin pen is prevented from being damaged, and damping protection performance is high.